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Glycoconjugate Journal

, Volume 25, Issue 9, pp 851–861 | Cite as

Structural characterisation and biological activities of a unique type β-d-glucan obtained from Aureobasidium pullulans

  • Rui Tada
  • Asuka Tanioka
  • Haruyo Iwasawa
  • Kumi Hatashima
  • Yoshikazu Shoji
  • Ken-ichi Ishibashi
  • Yoshiyuki Adachi
  • Masatoshi Yamazaki
  • Kazufumi Tsubaki
  • Naohito OhnoEmail author
Article

Abstract

A β-d-glucan obtained from Aureobasidium pullulans (AP-FBG) exhibits various biological activities: it exhibits antitumour and antiosteoporotic effects and prevents food allergies. An unambiguous structural characterisation of AP-FBG is still awaited. The biological effects of β-d-glucan are known to depend on its primary structures, conformation, and molecular weight. Here, we elucidate the primary structure of AP-FBG by NMR spectroscopy, and evaluate its biological activities. Its structure was shown to comprise a mixture of a 1-3-β-d-glucan backbone with single 1-6-β-d-glucopyranosyl side-branching units every two residues (major structure) and a 1-3-β-d-glucan backbone with single 1-6-β-d-glucopyranosyl side-branching units every three residues (minor structure). Furthermore, this β-d-glucan exhibited immunostimulatory effects such as the accumulation of immune cells and priming effects against enterobacterium. To our knowledge, 1-3-β-glucans like AP-FBG with such a high number of 1-6-β-glucopyranosyl side branching have a unique structure; nevertheless, many 1-3-β-glucans were isolated from various sources, e.g. fungi, bacteria, and plants.

Keywords

Aureobasidium pullulans β-d-glucan Structure NMR Biological activity 

Abbreviations

AP-FBG

Aureobasidium pullulans-fermented β-glucan

BRM

biological response modifier

CR3

complement receptor type 3

COSY

correlation spectroscopy

CSBG

β-glucan from Candida albicans

DB

degree of branching

DP

degree of polymerisation

Enterococcus faecalis

E. faecalis

ELISA

enzyme-linked immunosorbent assay

FCS

foetal calf serum

HMBC

heteronuclear multiple bond correlation spectroscopy

HSQC

heteronuclear single quantum correlation spectroscopy

H2BC

heteronuclear 2-bond correlation spectroscopy

IFN

interferon

IL

interleukin

LacCer

lactosylceramide

NMR

nuclear magnetic resonance

NOESY

nuclear Overhauser effect spectroscopy

PAMPs

pathogen associated molecular patterns

PBMCs

peripheral blood mononuclear cells

PBS

phosphate-buffered saline

PBST

0.05% Tween 20 containing PBS

BPBST

1% BSA containing PBST

PECs

peritoneal exudate cells

PMN

polymorphonuclear cell

PRRs

pattern recognition receptors

ROE

rotating-frame Overhauser effect

SPG

sonifilan

TLRs

toll-like receptors

TNF

tumour necrosis factor

TOCSY

total correlation spectroscopy

Notes

Acknowledgements

We would like to express our sincere appreciation to Misato Ebara for her consistent help and support. This work was supported by a grant for private universities provided by the Ministry of Education, Culture, Sports, Science, and Technology and Japan Private School Promotion Foundation.

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Copyright information

© Springer Science+Business Media, LLC 2008

Authors and Affiliations

  • Rui Tada
    • 1
  • Asuka Tanioka
    • 2
  • Haruyo Iwasawa
    • 3
  • Kumi Hatashima
    • 2
  • Yoshikazu Shoji
    • 2
  • Ken-ichi Ishibashi
    • 1
  • Yoshiyuki Adachi
    • 1
  • Masatoshi Yamazaki
    • 3
  • Kazufumi Tsubaki
    • 2
  • Naohito Ohno
    • 1
    Email author
  1. 1.Laboratory for Immunopharmacology of Microbial Products, School of PharmacyTokyo University of Pharmacy and Life SciencesTokyoJapan
  2. 2.Advance Materials R&D Laboratory, Life Sciences Department, ADEKA CorporationTokyoJapan
  3. 3.Faculty of Pharmaceutical SciencesTeikyo UniversityKanagawaJapan

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