Glycoconjugate Journal

, Volume 25, Issue 9, pp 851–861 | Cite as

Structural characterisation and biological activities of a unique type β-d-glucan obtained from Aureobasidium pullulans

  • Rui Tada
  • Asuka Tanioka
  • Haruyo Iwasawa
  • Kumi Hatashima
  • Yoshikazu Shoji
  • Ken-ichi Ishibashi
  • Yoshiyuki Adachi
  • Masatoshi Yamazaki
  • Kazufumi Tsubaki
  • Naohito OhnoEmail author


A β-d-glucan obtained from Aureobasidium pullulans (AP-FBG) exhibits various biological activities: it exhibits antitumour and antiosteoporotic effects and prevents food allergies. An unambiguous structural characterisation of AP-FBG is still awaited. The biological effects of β-d-glucan are known to depend on its primary structures, conformation, and molecular weight. Here, we elucidate the primary structure of AP-FBG by NMR spectroscopy, and evaluate its biological activities. Its structure was shown to comprise a mixture of a 1-3-β-d-glucan backbone with single 1-6-β-d-glucopyranosyl side-branching units every two residues (major structure) and a 1-3-β-d-glucan backbone with single 1-6-β-d-glucopyranosyl side-branching units every three residues (minor structure). Furthermore, this β-d-glucan exhibited immunostimulatory effects such as the accumulation of immune cells and priming effects against enterobacterium. To our knowledge, 1-3-β-glucans like AP-FBG with such a high number of 1-6-β-glucopyranosyl side branching have a unique structure; nevertheless, many 1-3-β-glucans were isolated from various sources, e.g. fungi, bacteria, and plants.


Aureobasidium pullulans β-d-glucan Structure NMR Biological activity 



Aureobasidium pullulans-fermented β-glucan


biological response modifier


complement receptor type 3


correlation spectroscopy


β-glucan from Candida albicans


degree of branching


degree of polymerisation

Enterococcus faecalis

E. faecalis


enzyme-linked immunosorbent assay


foetal calf serum


heteronuclear multiple bond correlation spectroscopy


heteronuclear single quantum correlation spectroscopy


heteronuclear 2-bond correlation spectroscopy








nuclear magnetic resonance


nuclear Overhauser effect spectroscopy


pathogen associated molecular patterns


peripheral blood mononuclear cells


phosphate-buffered saline


0.05% Tween 20 containing PBS


1% BSA containing PBST


peritoneal exudate cells


polymorphonuclear cell


pattern recognition receptors


rotating-frame Overhauser effect




toll-like receptors


tumour necrosis factor


total correlation spectroscopy



We would like to express our sincere appreciation to Misato Ebara for her consistent help and support. This work was supported by a grant for private universities provided by the Ministry of Education, Culture, Sports, Science, and Technology and Japan Private School Promotion Foundation.


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Copyright information

© Springer Science+Business Media, LLC 2008

Authors and Affiliations

  • Rui Tada
    • 1
  • Asuka Tanioka
    • 2
  • Haruyo Iwasawa
    • 3
  • Kumi Hatashima
    • 2
  • Yoshikazu Shoji
    • 2
  • Ken-ichi Ishibashi
    • 1
  • Yoshiyuki Adachi
    • 1
  • Masatoshi Yamazaki
    • 3
  • Kazufumi Tsubaki
    • 2
  • Naohito Ohno
    • 1
    Email author
  1. 1.Laboratory for Immunopharmacology of Microbial Products, School of PharmacyTokyo University of Pharmacy and Life SciencesTokyoJapan
  2. 2.Advance Materials R&D Laboratory, Life Sciences Department, ADEKA CorporationTokyoJapan
  3. 3.Faculty of Pharmaceutical SciencesTeikyo UniversityKanagawaJapan

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