Glycoconjugate Journal

, Volume 23, Issue 3–4, pp 167–173 | Cite as

Altered expression and glycosylation of plasma proteins in rheumatoid arthritis

  • Sunil K. Raghav
  • Bhawna Gupta
  • Charu Agrawal
  • Ashish Saroha
  • Rakha H. Das
  • Ved P. Chaturvedi
  • Hasi R. Das

Abstract

Altered glycosylation of plasma proteins has been directly implicated in the pathogenesis of rheumatoid arthritis (RA). The present study investigated the changes in the Concanavalin-A (Con-A)-bound plasma proteins in the RA patients in comparison to that of the healthy controls. Two proteins (MW ∼32 kDa and ∼62 kDa) showed an alteration in expression while an altered monosaccharide profile (high mannose) was observed in the ∼62 kDa protein in the samples collected from RA patients. The 2-dimensional polyacrylamide gel electrophoresis analysis of the Con-A-bound plasma samples showed a large number of protein spots, a few of which were differentially expressed in the RA patients. Some unidentified proteins were detected in the RA patients which were absent in the control samples. The present study, therefore, enunciates the role of carbohydrates as well as that of the acute phase response in the disease pathogenesis.

Keywords

Rheumatoid arthritis Concanavalin A Altered glycosylation Plasma proteins 2-dimensional gel electrophoresis 

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Copyright information

© Springer Science + Business Media, LLC 2006

Authors and Affiliations

  • Sunil K. Raghav
    • 1
  • Bhawna Gupta
    • 1
  • Charu Agrawal
    • 1
  • Ashish Saroha
    • 1
  • Rakha H. Das
    • 1
  • Ved P. Chaturvedi
    • 2
  • Hasi R. Das
    • 1
  1. 1.Institute of Genomics & Integrative BiologyDelhiIndia
  2. 2.Department of Rheumatology, R&RArmy HospitalN. DelhiIndia

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