Glycoconjugate Journal

, Volume 23, Issue 5–6, pp 443–452 | Cite as

Preferential reduction of the α-2-6-sialylation from cell surface N-glycans of human diploid fibroblastic cells by in vitro aging

  • Tomomi Tadokoro
  • Kiyotaka Yamamoto
  • Iku Kuwahara
  • Hirosuke Fujisawa
  • Masahiko Ikekita
  • Akiyoshi Taniguchi
  • Takeshi Sato
  • Kiyoshi Furukawa
Original Papers


Human diploid fibroblastic cell line, TIG-3, has a finite life span of about 80 population doubling levels (PDL), and is used for in vitro aging studies. Young cells (PDL 23) grew to higher cell densities at a higher growth rate than aged cells (PDL 77). When the electrophoretic mobility of cells was determined, the negative surface charge of the aged cells decreased significantly when compared to that of young cells. Lectin blot analysis of membrane glycoproteins showed that the α-2-6-sialylation but not the α-2-3-sialylation of N-glycans decreases markedly in the aged cells when compared to the young cells. In support of this observation, the cDNA microarray assay and reverse transcription-polymerase chain reaction (RT-PCR) analysis showed that the gene expression of the α-2,6-sialyltransferase I (ST6Gal I), which transfers sialic acid to galactose residues of N-glycans, decreases in the aged cells. These results indicate that the concordant decrease of the α-2,6-sialylation of N-glycans with the ST6Gal I gene expression is induced in TIG-3 cells by in vitro aging.


N-glycans Sialylation ST6Gal I TIG-3 cells In vitroaging 



Coomassie Brilliant Blue

Con A

concanavalin A


doubling time


ethidium bromide






1,6-Man β-1,2-N-acetyl-glucosaminyltransferase


glyceraldehyde 3-phosphate dehydrogenase


fetal calf serum


Lens culinaris agglutinin


leuko-agglutinating phytohemagglutinin


Maackia amurensis agglutinin


2-(N-morpholino)ethansulfonic acid


phosphate-buffered saline


population doubling levels


Psathyrella velutina lectin


Ricinus communis agglutinin-I


reverse transcription polymerase chain reaction


Sambucus nigra agglutinin


β-galactoside α-2,3-sialyltransferase III


β-galactoside α-2,3-sialytransferase IV

ST6Gal I

β-galactoside α-2,6-sialyltransferase I


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Copyright information

© Springer Science + Business Media, LLC 2006

Authors and Affiliations

  • Tomomi Tadokoro
    • 1
    • 2
  • Kiyotaka Yamamoto
    • 1
  • Iku Kuwahara
    • 1
    • 3
  • Hirosuke Fujisawa
    • 3
  • Masahiko Ikekita
    • 2
  • Akiyoshi Taniguchi
    • 4
  • Takeshi Sato
    • 1
    • 5
  • Kiyoshi Furukawa
    • 1
    • 5
  1. 1.Department of Biosignal ResearchTokyo Metropolitan Institute of GerontologyTokyoJapan
  2. 2.Department of Applied Biological Science, Faculty of Science and TechnologyTokyo University of ScienceNodaJapan
  3. 3.Faculty of Education, Institute of Natural SciencesSaitama UniversitySaitamaJapan
  4. 4.Biomaterials Center, National Institute For Materials ScienceTsukubaJapan
  5. 5.Laboratory of GlycobiologyNagaoka University of TechnologyNagaokaJapan

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