Glycoconjugate Journal

, Volume 23, Issue 7–8, pp 463–471 | Cite as

Galactosylation of IgG from rheumatoid arthritis (RA) patients – changes during therapy

  • Marta Pasek
  • Maria Duk
  • Maria Podbielska
  • Renata Sokolik
  • Jacek Szechiński
  • Elwira Lisowska
  • Hubert KrotkiewskiEmail author


It is well documented that serum IgG from rheumatoid arthritis (RA) patients exhibits decreased galactosylation of its conservative N-glycans (Asn-297) in CH2 domains of the heavy chains; it has been shown that this agalactosylation is proportional to disease severity. In the present investigation we analyzed galactosylation of IgG derived from the patients using a modified ELISA-plate test, biosensor BIAcore and total sugar analysis (GC-MS). For ELISA and BIAcore the binding of IgG preparations, purified from the patients’ sera, to two lectins: Ricinus communis (RCA-I) and Griffonia simplicifolia (GSL-II) was applied. Based on ELISA-plate test an agalactosylation factor (AF, a relative ratio of GSL-II/RCA-I binding) was calculated, which was proportional to actual disease severity. Repeated testing of several patients before and after treatment with methotrexate (MTX) alone or in combination with Remicade (a chimeric antibody anti-TNF-α) supplied results indicating an increase of IgG galactosylation during the treatment. This introductory observation suggests that IgG galactosylation may be an additional indicator of the RA patients’ improvement.


IgG Rheumatoid arthritis Surface plasmon resonance Ricinus communis lectin Griffonia simplicifolia lectin 



immunoglobulin G


rheumatoid arthritis


lectin from Ricinus communis


lectin from Psathyrella velutina


lectin from Griffonia simplicifolia


erythrocyte sedimentation rate


C-reactive protein


gas chromatography-mass spectrometry








agalactosylation factor


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Copyright information

© Springer Science + Business Media, LLC 2006

Authors and Affiliations

  • Marta Pasek
    • 1
  • Maria Duk
    • 1
  • Maria Podbielska
    • 1
  • Renata Sokolik
    • 2
  • Jacek Szechiński
    • 2
  • Elwira Lisowska
    • 1
  • Hubert Krotkiewski
    • 1
    Email author
  1. 1.Ludwik Hirszfeld Institute of Immunology & Experimental TherapyPolish Academy of SciencesWroclawPoland
  2. 2.Department of RheumatologyMedical UniversityWroclawPoland

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