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Genetica

, Volume 145, Issue 2, pp 235–240 | Cite as

Meiotic pairing as an indicator of genome composition in polyploid prairie cordgrass (Spartina pectinata Link)

  • Jeffrey W. Bishop
  • Sumin Kim
  • María B. Villamil
  • D. K. Lee
  • A. Lane RayburnEmail author
Short Communication

Abstract

The existence of neopolyploidy in prairie cordgrass (Spartina pectinata Link) has been documented. The neohexaploid was discovered coexisting with tetraploids in central Illinois, and has been reported to exhibit competitiveness in the natural environment. It is hypothesized that the natural tetraploid cytotype produced the hexaploid cytotype via production of unreduced gametes. Meiosis I chromosome pairing was observed in tetraploid (2n = 4x = 40), hexaploid (2n = 6x = 60), and octoploid (2n = 8x = 80) accessions and the percentage of meiotic abnormality was determined. Significant differences in meiotic abnormality exist between tetraploid, hexaploid, and octoploid cytotypes. An elevated incidence of abnormal, predominantly trivalent pairing in the neohexaploid suggests that it may possess homologous chromosomes in sets of three, in contrast to the tetraploid and octoploid cytotypes, which likely possess homologous chromosomes in sets of two. Abnormal chromosome pairing in the hexaploid may result in unequal allocation of chromosomes to daughter cells during later stages of meiosis. Chromosome pairing patterns in tetraploid, hexaploid, and octoploid cytotypes indicate genome compositions of AABB, AAABBB, and AABBA′A′B′B′, respectively.

Keywords

Chromosomes Homology Polyploidy Meiosis 

Notes

Acknowledgements

This work was supported by funding from the North Central Regional Sun Grant Center at South Dakota State University through a grant provided by the United States Department of Agriculture (Award Number 2010-38502-21861) and by the National Institute of Food and Agriculture, U.S. Department of Agriculture, Hatch project under ILLU-802-952.

Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflict of interest.

References

  1. Attia T, Busso C, Röbbelen G (1987) Digenomic triploids for an assessment of chromosome relationships in the cultivated diploid Brassica species. Genome 29:326–330CrossRefGoogle Scholar
  2. Church GL (1940) Cytotaxonomic studies in the Graminae Spartina, Andropogon, and Panicum. Am J Bot 27:263–271CrossRefGoogle Scholar
  3. Crawford J, Brown PJ, Voigt T, Lee DK (2016) Linkage mapping in prairie cordgrass (Spartina pectinata Link) using genotyping-by-sequencing. Mol Breeding 36:62. doi: 10.1007/s11032-016-0484-9 CrossRefGoogle Scholar
  4. Doyle JJ, Egan AN (2010) Dating the origins of polyploidy events. New Phytol 186:73–85CrossRefPubMedGoogle Scholar
  5. Doyle JJ, Flagel LE, Paterson AH, Rapp RA, Soltis DE, Soltis PS, Wendel JF (2008) Evolutionary genetics of genome merger and doubling in plants. Annu Rev Genet 42:443–461CrossRefPubMedGoogle Scholar
  6. Evans GM, Macefield AJ (1972) The suppression of homoeologous pairing by B-chromosomes in a Lolium species hybrid. Nature 236:110–111CrossRefGoogle Scholar
  7. Fortune PM, Schierenbeck KA, Ainouche AK, Jacquemin J, Wendel JF, Ainouche ML (2007) Evolutionary dynamics of Waxy and the origin of hexaploid Spartina species (Poaceae). Mol Phylogenet Evol 43:1040–1055CrossRefPubMedGoogle Scholar
  8. Graves H, Rayburn AL, Gonzales-Hernandez JL, Nah G, Kim D, Lee DK (2015) Validating DNA polymorphisms using KASP assay in prairie cordgrass (Spartina pectinata Link) populations in the US. Front Plant Sci 6:1271. doi: 10.3389/fpls.2015.01271 PubMedGoogle Scholar
  9. Graves H, Rayburn AL, Kim S, Lee DK (2016) Chloroplast DNA variation within prairie cordgrass (Spartina pectinata Link) populations in the US. J Syst Evol 54(2):104–112CrossRefGoogle Scholar
  10. Heyne EG (1987) Wheat and wheat improvement. American Society of Agronomy, Inc.; Crop Science Society of America, Inc.; Soil Science Society of America, Inc; Madison, WI, USAGoogle Scholar
  11. Jackson RC (1982) Polyploidy and diploidy: new perspectives on chromosome pairing and its evolutionary implications. Am J Bot 69(9):1512–1523CrossRefGoogle Scholar
  12. Kim S, Rayburn AL, Lee DK (2010) Genome size and chromosome analyses in prairie cordgrass. Crop Sci 50:2277–2282CrossRefGoogle Scholar
  13. Kim S, Rayburn AL, Boe A, Lee DK (2012a) Neopolyploidy in Spartina pectinata Link: 1. Morphology analysis of tetraploid and hexaploid plants in a mixed natural population. Mol Phylogenet Evol 298:1073–1083Google Scholar
  14. Kim S, Rayburn AL, Parrish A, Lee DK (2012b) Cytogeographic distribution and genome size variation in prairie cordgrass (Spartina pectinata Bosc ex Link). Plant Mol Biol Rep 30:1073–1079CrossRefGoogle Scholar
  15. Kim S, Rayburn AL, Voigt TB, Ainouche ML, Ainouche AK, Lee DK (2013) Chloroplast DNA intraspecific phylogeography of prairie cordgrass (Spartina pectinata Bosc ex Link). Plant Mol Biol Rep 31:1376–1383CrossRefGoogle Scholar
  16. Krahulcová A, Krahulec F (2000) Offspring diversity in Hieracium subgen. pilosella (Asteraceae): new cytotypes from hybridization experiments and from open pollination. Fragm Flor Geobot 45:239–255Google Scholar
  17. Levin DA (2002) The role of chromosomal change in plant evolution. Oxford University Press, OxfordGoogle Scholar
  18. Love RM (1951) Varietal differences in meiotic chromosome behavior of Brazilian wheats. Agron J 43:72–76CrossRefGoogle Scholar
  19. Marchant CJ (1968) Evolution in Spartina (Graminae) III. Species chromosome numbers and their taxonomic significance. J Linn Soc Bot 60(383):411–417CrossRefGoogle Scholar
  20. Rajhathy T (1971) The allopolyploid model in Avena. In: G. Kimber, G.P. Redei (eds). Stadler Symposia 3:71–87Google Scholar
  21. Ramsey J, Schemske DW (2002) Neopolyploidy in flowering plants. Annu Rev Ecol Evol Syst 33:589–639CrossRefGoogle Scholar
  22. Reeder J (1977) Chromosome numbers in western grasses. Am J Bot 64(1):102–110CrossRefGoogle Scholar
  23. Snow R (1963) Alcoholic hydrochloric acid-carmine as a stain for chromosomes in squash preparations. Stain Technol 38:9–13CrossRefPubMedGoogle Scholar
  24. United States Department of Agriculture, Natural Resources Conservation Service (2002) Prairie cordgrass plant guide. Natural Resources Conservation Service, Washington, DC. http://plants.usda.gov/plantguide/pdf/pg_sppe.pdf. Accessed 13 Feb 2014
  25. Wang RRC, Jensen KB (1994) Absence of the J genome in Leymus species (Poaceae: Triticeae): evidence from DNA hybridization and meiotic pairing. Genome 37:231–235CrossRefPubMedGoogle Scholar

Copyright information

© Springer International Publishing Switzerland 2017

Authors and Affiliations

  • Jeffrey W. Bishop
    • 1
  • Sumin Kim
    • 1
  • María B. Villamil
    • 1
  • D. K. Lee
    • 1
  • A. Lane Rayburn
    • 1
    Email author
  1. 1.Department of Crop SciencesUniversity of IllinoisUrbanaUSA

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