Genetica

, Volume 146, Issue 1, pp 115–121 | Cite as

Development and characterization of 79 nuclear markers amplifying in viviparous and oviparous clades of the European common lizard

  • J. L. Horreo
  • M. L. Peláez
  • T. Suárez
  • P. S. Fitze
Short Communication
First Online:
Received:
Accepted:

Abstract

The European common lizard (Zootoca vivipara) is a widely distributed species across Europe and Asia exhibiting two reproductive modes (oviparity/viviparity), six major lineages and several sublineages. It has been used to tackle a large variety of research questions, nevertheless, few nuclear DNA sequence markers have been developed for this species. Here we developed 79 new nuclear DNA sequence markers using a clonation protocol. These markers were amplified in several oviparous and viviparous specimens including samples of all extant clades, to test the amplification success and their diversity. 49.4% of the markers were polymorphic and of those, 51.3% amplified in all and 94.9% amplified in 5–7 of the extant Z. vivipara clades. These new markers will be very useful for the study of the population structure, population dynamics, and micro/macro evolution of Z. vivipara. Cross-species amplification in four lizard species (Psammodromus edwardsianus, Podarcis muralis, Lacerta bilineata, and Takydromus sexlineatus) was positive in several of the markers, and six makers amplified in all five species. The large genetic distance between P. edwardsianus and Z. vivipara further suggests that these markers may as well be employed in many other species.

Keywords

Zootoca vivipara Lacertidae Nuclear DNA Clonation Reptile Representative genes 
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Notes

Acknowledgements

We are grateful to Benoit Heulin and Werner Mayer, who provided us with samples of the Easter and Central European clades.

Funding

This study was funded by a EU Marie Curie-Clarín CoFound (Grant Number ACA14-26 to J. L. H.), the Spanish MINECO postdoc grants (FPDI-2013-16116, IJCI-2015-23618 to J. L. H.), the Swiss National Science Foundation (PPOOP3_128375 and PP00P3_152929/1 to P. S. F.), and the Spanish Ministry of Education and Science (CGL2008-01522, CGL2012-32459, CGL2016-76918-P to P. S. F.).

Compliance with ethical standards

Conflict of interest

All the authors declare no conflict of interests.

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Copyright information

© Springer International Publishing AG, part of Springer Nature 2017

Authors and Affiliations

  1. 1.Department of Ecology and Evolution (DEE)University of LausanneLausanneSwitzerland
  2. 2.Department of Biodiversity and Ecologic RestorationInstituto Pirenaico de Ecología (IPE-CSIC)JacaSpain
  3. 3.Department of Biodiversity and Evolutionary BiologyMuseo Nacional de Ciencias Naturales (MNCN-CSIC)MadridSpain
  4. 4.Department of Cellular and Molecular PhysiopathologyCentro de Investigaciones Biologicas (CIB-CSIC)MadridSpain
  5. 5.Fundación Araid, Edificio CEEI AragónZaragozaSpain

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