Development and characterization of twelve microsatellite markers for Porphyra linearis Greville
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The genus Porphyra (and its sister genus Pyropia) contains important red algal species that are cultivated and/or harvested for human consumption, sustaining a billion-dollar aquaculture industry. A vast amount of research has been focused on species of this genus, including studies on genetics and genomics among other areas. Twelve novel microsatellite markers were developed here for Porphyra linearis. Markers were characterized using 32 individuals collected from four natural populations of P. linearis with total heterozygosity varying from 0.098 to 0.916. The number of alleles per locus ranged from 2 to 18. All markers showed cross amplification with Porphyra umbilicalis and/or Porphyra dioica. These polymorphic microsatellite markers are useful for investigating population genetic diversity and differentiation in P. linearis and may become useful for other genetic research on the reproductive biology of this important species.
KeywordsPorphyra Microsatellite markers Red algae Population structure North Atlantic
Funded by FCT (Fundação para a Ciência e a Tecnologia) Postdoctoral fellowship (SFRH/BPD/109452/2015) to EVÁ, and the following FCT Projects: (NORIGENOMICS—PTDC/MAR/099698/2008) to EVÁ, EXTANT—EXCL/AAG-GLO/0661/2012 to ES, and UID/Multi/04326/2013 to CCMAR. We also would like to thank (1) Travis Glenn for the help/comments with the design of the probes for the enrichments; (2) Mafalda Afonso and Ana Balau for laboratory work; (3) Henning Steen (Havforskningsinstituttet, Institute of Marine Research, Norway) and (4) ASSEMBLE network (European Community) by the Remote access platform (ASSEMBLE Grant Agreement No. 227799) for providing samples; and (4) the following people who helped with sample collection: Liam Cronin, Ignacio Bárbara, Estibaliz Berecibar, Tânia Pereira, Dagmar Stengel, Anna Soler, and Fabio Rindi.
Compliance with ethical standards
Conflict of interest
The authors declare that they have no conflict of interest.
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