Advertisement

Genetica

, Volume 144, Issue 5, pp 601–609 | Cite as

Aneuploid progenies of triploid hybrids between diploid and tetraploid loach Misgurnus anguillicaudatus in China

  • Ya-Juan Li
  • Yang-Chun Gao
  • He Zhou
  • Hai-Yan Ma
  • Zhong-Qiao Lin
  • Tian-Yu Ma
  • Yi Sui
  • Katsutoshi Arai
Article

Abstract

Triploid Chinese loach, Misgurnus anguillicaudatus, hybrids between tetraploids from Hubei Province and diploids from Liaoning Province were mated with either diploid wild-type or triploid hybrids to analyze viability and ploidy of the resultant progenies. Both triploid males and females generated fertile gametes, but progenies from the crosses using gametes of triploid hybrids did not survive beyond the larval stages. In crosses between wild-type diploid females and triploid hybrid males, embryos ranging from 2.2n to 2.6n were predominant with a mode of either 2.4n (chromosome numbers 59, 60, 61) or 2.5n (chromosome numbers 62, 63). Those from the crosses between triploid hybrid females and diploid males gave a modal ploidy level at approximately 2.5n in one case, but a shift to a higher ploidy level was observed in other embryos. In the progenies between triploid hybrid females and males, the ploidy level at approximately 3.0n (chromosome numbers 74, 75, 76) was most frequent. The cytogenetic results of the progenies suggest the production of aneuploid gametes with a modal ploidy level at approximately 1.5n in triploid hybrids. However, a shift to higher chromosome numbers in gametes was observed in certain cases, suggesting the involvement of mortality selection of gametes and/or zygotes with lower chromosome numbers.

Keywords

Bivalent Gamete Meiosis Polyploid Trivalent Univalent 

Abbreviation

Ag-NORs

silver staining nucleolus organizer regions.

CMA3

chromomycin A3.

CN

chromosome number.

DA

distamycin A.

DAPI

4′6-diamidino-2-phenylindole.

FISH

fluorescence in situ hybridization.

Notes

Acknowledgments

This study was supported in part by KAKENHI Grant-in-Aid for Scientific Research (B) (No.21380114) from the Japan Society of the Promotion of Science (JSPS) to KA and Grants-in-Aid from the National Natural Science Foundation of China (No.31272650) and the Natural Science Foundation of Liaoning Province (No.201102019) to YJL.

Supplementary material

10709_2016_9928_MOESM1_ESM.ppt (322 kb)
Supplementary material 1 (PPT 321 kb)
10709_2016_9928_MOESM2_ESM.ppt (365 kb)
Supplementary material 2 (PPT 365 kb)
10709_2016_9928_MOESM3_ESM.xls (34 kb)
Supplementary material 3 (XLS 34 kb)
10709_2016_9928_MOESM4_ESM.xls (48 kb)
Supplementary material 4 (XLS 48 kb)
10709_2016_9928_MOESM5_ESM.xls (46 kb)
Supplementary material 5 (XLS 46 kb)
10709_2016_9928_MOESM6_ESM.xls (35 kb)
Supplementary material 6 (XLS 35 kb)
10709_2016_9928_MOESM7_ESM.xlsx (14 kb)
Supplementary material 7 (XLSX 14 kb)
10709_2016_9928_MOESM8_ESM.xls (29 kb)
Supplementary material 8 (XLS 29 kb)
10709_2016_9928_MOESM9_ESM.xlsx (14 kb)
Supplementary material 9 (XLSX 14 kb)

References

  1. Allen SK, Thiery RG, Hagstrom NT (1986) Cytological evaluation of the likelihood that triploid grass carp will reproduce. Trans Am Fish Soc 115:841–848CrossRefGoogle Scholar
  2. Arai K, Inamori Y (1999) Viable hyperdiploid progeny between diploid female and induced triploid male in the loach Misgurnus anguillicaudatus. Aquacul Sci 47:489–495Google Scholar
  3. Arai K, Mukaino M (1997) Clonal nature of gynogenetically induced progeny of triploid (diploid × tetraploid) loach M. anguillicaudatus (Pisces: Cobitididae). J Exp Zool 278:412–420CrossRefGoogle Scholar
  4. Arai K, Mukaino M (1998) Electrophoretic analysis of the diploid progenies from triploid × diploid crosses in the loach M. anguillicaudatus (Pisces: Cobitidae). J Exp Zool 280:368–374CrossRefGoogle Scholar
  5. Benfey TJ, Solar II, de Jong G, Donaldson EM (1986) Flow-cytometric confirmation of aneuploidy in sperm from triploid rainbow trout. Trans Am Fish Soc 115:838–840CrossRefGoogle Scholar
  6. Fankhauser G, Hamphrey RR (1950) Chromosome number and development of progeny of triploid axolotl females mated with diploid males. J Exp Zool 115:207–250CrossRefGoogle Scholar
  7. Fankhauser G, Hamphrey RR (1954) Chromosome number and development of progeny of triploid axolotl males crossed with diploid females. J Exp Zool 126:33–58CrossRefGoogle Scholar
  8. Fukui K, Tsujimoto H (2010) Cytogenetics in breeding. Yokendo, Tokyo (in Japanese) Google Scholar
  9. Gomelsky B, Schneider KJ, Anil A, Delomas TA (2015) Gonad development in triploid ornamental koi carp and results of crossing triploid females with diploid males. North Am J Aquacul 77:96–101CrossRefGoogle Scholar
  10. Howell WM, Black DA (1980) Controlled silver-staining of nucleolus organizer regions with a protective colloidal developer: a 1-step method. Experientia 36:1014–1015CrossRefPubMedGoogle Scholar
  11. Levan A, Fredga K, Sandberg AA (1964) Nomenclature for centromeric position on chromosomes. Hereditas 52:201–220CrossRefGoogle Scholar
  12. Li YJ, Yin J, Wang JB, Yuan X, Wei J, Sun XW, Arai K (2008) A Study on the distribution of polyploid loaches in China. Nippon Suisan Gakkaishi 74:177–182CrossRefGoogle Scholar
  13. Li YJ, Tian Y, Zhang MZ, Tian PP, Yu Z, Abe S, Arai K (2010) Chromosome banding and FISH with an rDNA probe in the diploid and tetraploid Loach, M. anguillicaudatus. Ichthyol Res 57(4):358–366CrossRefGoogle Scholar
  14. Li YJ, Yu Z, Zhang MZ, Qian C, Abe S, Arai K (2011) The origin of natural tetraploid loach M. anguillicaudatus (Teleostei: Cobitidae) inferred from meiotic chromosome configurations. Genetica 139:805–811CrossRefPubMedGoogle Scholar
  15. Li YJ, Zhang MZ, Qin C, Gao M, Arai K (2012) Fertility and ploidy of gametes of diploid, triploid and tetraploid loaches, M. anguillicaudatus, in China. J Appl Ichthyol 28:900–905CrossRefGoogle Scholar
  16. Li YJ, Yu Z, Zhang MZ, Qian C, Abe S, Arai K (2013) Induction and viable gynogenetic progeny using eggs and UV-irradiated sperm from the Chinese tetraploid loach, M. anguillicaudatus. Aquacult Int 21:759–768CrossRefGoogle Scholar
  17. Li YJ, Gao YC, Zhou H, Ma HY, Li JQ, Arai K (2015) Meiotic chromosome configurations in triploid progeny from reciprocal crosses between wild-type diploid and natural tetraploid loach M. anguillicaudatus in China. Genetica 143:555–562CrossRefPubMedGoogle Scholar
  18. Matsubara K, Arai K, Suzuki R (1995) Survival potential and chromosomes of progeny of triploid and pentaploid females in the loach M. anguillicaudatus. Aquaculture 131:37–48CrossRefGoogle Scholar
  19. Morishima K, Yoshikawa H, Arai K (2008) Meiotic hybridogenesis in triploid Misgurnus loach derived from a clonal lineage. Heredity 100:581–586CrossRefPubMedGoogle Scholar
  20. Oshima K, Morishima K, Yamaha E, Arai K (2005) Reproductive capacity of triploid loaches obtained from Hokkaido Island, Japan. Ichthyol Res 52:1–8CrossRefGoogle Scholar
  21. Schweizer D (1976) Reverse fluorescent chromosome banding with chromomycin and DAPI. Chromosoma (Berl) 58:307–324CrossRefGoogle Scholar
  22. Schweizer D, Ambros P, Andrle M (1978) Modification of DAPI banding on human chromosomes by prestaining with a DNA-binding oligopeptide antibiotic, distamycin A. Exp Cell Res 111:327–332CrossRefPubMedGoogle Scholar
  23. Ueda T, Sawada M, Kobayashi J (1987) Cytogenetical characteristics of the embryos between diploid female and triploid male in rainbow trout. Jpn J Genet 62:461–465CrossRefGoogle Scholar
  24. Ueda T, Sato R, Iwata M, Komaru A, Kobayashi J (1991) The viable 3.5n routs produced between diploid females and allotriploid males. Jpn J Genet 66:71–75CrossRefGoogle Scholar
  25. Van Eenennaam JP, Stocker RK, Thiery RG, Hagstrom NT, Doroshov SI (1990) Egg fertility, early developmenta and survival from crosses of diploid female × triploid male Grass Carp (Ctenopharyngodon idella). Aquaculture 86:111–125CrossRefGoogle Scholar
  26. Yoshikawa H, Morishima K, Kusuda S, Yamaha E, Arai K (2007) Diploid sperm produced by artificially sex-reversed clone loaches. J Exp Zool 307A:75–83CrossRefGoogle Scholar
  27. Zhang Q, Arai K (1999) Aberrantmeioses and viable aneuploidy progeny of induced triploid (M. anguillicaudatus) when crossed to natural tetraploids. Aquaculture 175:63–76CrossRefGoogle Scholar
  28. Zhang Q, Arai K, Yamashita M (1998) Cytogenetic mechanisms for triploid and haploid egg formation in thetriploid loach M. anguillicaudatus. J Exp Zool 281:608–619CrossRefGoogle Scholar

Copyright information

© Springer International Publishing Switzerland 2016

Authors and Affiliations

  • Ya-Juan Li
    • 1
  • Yang-Chun Gao
    • 2
  • He Zhou
    • 1
  • Hai-Yan Ma
    • 1
  • Zhong-Qiao Lin
    • 1
  • Tian-Yu Ma
    • 1
  • Yi Sui
    • 1
  • Katsutoshi Arai
    • 3
  1. 1.Key Laboratory of Mariculture and Stock Enhancement in North China’s Sea, Ministry of AgricultureDalian Ocean UniversityDalianChina
  2. 2.Research Center for Eco-Environmental ScienceChinese Academy of ScienceBeijingChina
  3. 3.Faculty and Graduate School of Fisheries SciencesHokkaido UniversityHakodate, HokkaidoJapan

Personalised recommendations