Satellite DNA (satDNA) is a non-coding component of eukaryotic genomes, located mainly in heterochromatic regions. Relevance of satDNA began to emerge with accumulating evidence of its potential yet hardly comprehensible role that it can play in the genome of many organisms. We isolated the first satDNA of the codling moth (Cydia pomonella, Tortricidae, Lepidoptera), a species with holokinetic chromosomes and a single large heterochromatic element, the W chromosome in females. The satDNA, called CpSAT-1, is located on all chromosomes of the complement, although in different amounts. Surprisingly, the satellite is almost missing in the heterochromatic W chromosome. Additionally, we isolated mRNA from all developmental stages (1st–5th instar larva, pupa, adult), both sexes (adult male and female) and several tissues (Malpighian tubules, gut, heart, testes, and ovaries) of the codling moth and showed the CpSAT-1 sequence was transcribed in all tested samples. Using CpSAT-1 specific primers we amplified, cloned and sequenced 40 monomers from cDNA and gDNA, respectively. The sequence analysis revealed a high mutation rate and the presence of potentially functional motifs, mainly in non-conserved regions of the monomers. Both the chromosomal distribution and the sequence analysis suggest that CPSAT-1 has no function in the C. pomonella genome.
Cydia pomonellaSatellite DNA Holokinetic chromosomes Sex chromosomes Lepidoptera
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We thank Marie Korchová for rearing of the codling moth and František Marec for critical reading of the manuscript. This research was funded by Grants KJB501410901 of the Grant Agency of the Academy of Sciences of the Czech Republic and 523/09/2106 of the Grant Agency of the Czech Republic (GACR). MD and MS were supported from GACR Grant 14-22765S. Additional support from grant 052/2013/P of the Grant Agency of the University of South Bohemia is also acknowledged. Finally, we wish to thank Juan Pedro M. Camacho, Francisco J. Ruiz-Ruano (both University of Granada, Spain), and two anonymous reviewers for valuable comments to the manuscript.
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Conflict of interest
The authors declare that they have no conflict of interest.
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