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Genetica

, Volume 141, Issue 7–9, pp 281–292 | Cite as

Taxonomic and population genetic re-interpretation of two color morphs of the decollate snail, Rumina decollata (Mollusca, Pulmonata) in southern France

  • Vanya Prévot
  • Kurt Jordaens
  • Natalie Van Houtte
  • Gontran Sonet
  • Kenny Janssens
  • Rita Castilho
  • Thierry Backeljau
Article

Abstract

The hermaphroditic terrestrial snail Rumina decollata has a mixed breeding system with a high prevalence of self-fertilization. In the Montpellier area (France), the species is represented by a dark and a light color morph. Based on allozyme data, both morphs have been reported as single, homozygous multilocus genotypes (MLG), differing at 13 out of 26 loci, but still showing occasional hybridization. Recent DNA sequence data suggest that each morph is a different phylogenetic species. In order to further evaluate this new taxonomic interpretation, the present contribution explores to what extent populations or color morphs indeed consist of single or few MLG. As such it is shown that both morphs are not single, homozygous MLG, but instead reveal a considerable amount of allelic variation and substantial numbers of heterozygous microsatellite genotypes. This suggests that outcrossing may be more prevalent than previously reported. Nevertheless, both morphs maintain a diagnostic multimarker differentiation in the presence of outcrossing in sympatric conditions, implying that they may be interpreted as species under the biological species concept. Finally, our data challenge the idea that simultaneous hermaphrodites should be either strict selfers or strict outcrossers.

Keywords

Allozymes Microsatellites DNA sequences Color polymorphism Population genetics Self-fertilization 

Notes

Acknowledgments

We are indebted to all the persons who collected specimens and helped us in various ways, as well as to Claúdia Patrão (University of Algarve, Portugal), Dr. Patrick Mardulyn (Free University of Brussels, Belgium) and to the Botanical Garden from the University of Montpellier. V. Prévot was a PhD fellow at the FNRS (Belgium). Financial support was provided by the “Fonds David et Alice Van Buuren” to VP and by BELSPO Action 1 project MO/36/017 to TB. This work was conducted within the framework of the BELSPO IUAP program “SPEEDY” and the FWO research community BeBOL.

Supplementary material

10709_2013_9727_MOESM1_ESM.doc (163 kb)
Supplementary material 1 (DOC 163 kb)

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Copyright information

© Springer Science+Business Media Dordrecht 2013

Authors and Affiliations

  • Vanya Prévot
    • 1
    • 2
  • Kurt Jordaens
    • 3
    • 4
  • Natalie Van Houtte
    • 3
  • Gontran Sonet
    • 4
  • Kenny Janssens
    • 1
  • Rita Castilho
    • 5
  • Thierry Backeljau
    • 1
    • 3
  1. 1.Department of Invertebrates and JEMURoyal Belgian Institute of Natural SciencesBrusselsBelgium
  2. 2.Laboratoire d’Evolution Biologique et EcologieUniversité Libre de Bruxelles (ULB)BrusselsBelgium
  3. 3.Evolutionary Ecology GroupUniversity of AntwerpAntwerpBelgium
  4. 4.Joint Experimental Molecular Unit (JEMU)Royal Museum for Central AfricaTervurenBelgium
  5. 5.Centro de Ciências do Mar (CCMAR)Universidade do AlgarveFaroPortugal

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