Genetica

, Volume 135, Issue 3, pp 257–265 | Cite as

Detection of quantitative trait loci for heading date based on the doubled haploid progeny of two elite Chinese wheat cultivars

  • Kunpu Zhang
  • Jichun Tian
  • Liang Zhao
  • Bin Liu
  • Guangfeng Chen
Article
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Abstract

Quantitative trait loci (QTLs) with epistatic and QTL × environment (QE) interaction for heading date were studied using a doubled haploid (DH) population containing 168 progeny lines derived from a cross between two elite Chinese wheat cultivars Huapei 3 × Yumai 57 (Triticum aestivum L.). A genetic map was constructed based on 305 marker loci, consisting of 283 SSR loci and 22 EST-SSR markers, which covered a total length of 2141.7 cM with an average distance of 7.02 cM between adjacent markers in the genome. QTL analyses were performed using a mixed linear model approach. Two main-effect QTLs and two pairs of digenic epistatic effects were detected for heading date on chromosomes 1B, 2B, 5D, 6D, 7A, and 7D at three different environments in 2005 and 2006 cropping seasons. A highly significant QTL with an F-value 148.96, designated as Qhd5D, was observed within the Xbarc320-Xwmc215 interval on chromosome 5DL, accounting for 53.19% of the phenotypic variance and reducing days-to-heading by 2.77 days. The Qhd5D closely links with a PCR marker Xwmc215 with the genetic distance 2.1 cM, which can be used in molecular marker-assisted selection (MAS) in wheat breeding programs. Moreover, the Qhd5D was located on the similar position of well-characterised vernalization sensitivity gene Vrn-D1. We are also spending more efforts to develop near-isogenic lines to finely map the Qhd5D and clone the gene Vrn-D1 through map-based cloning. The Qhd1B with additive effect on heading date has not been reported in previous linkage mapping studies, which might be a photoperiod-sensitive gene homoeologous to the Ppd-H2 gene on chromosome 1B. No main-effect QTLs for heading date were involved in epistatic effects.

Keywords

Digenic epistatic effect Doubled haploid population Heading date QTL × environment interaction Quantitative trait loci Wheat (Triticum aestivum L.) 
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Notes

Acknowledgements

This work was supported by the National Natural Science Foundation of China (30671270) and the Hi-Tech Research and Development (863) Program of China (2006AA100101 and 2006AA10Z1E9). The authors gratefully thank Professor Yan Hai (Henan Academy of Agricultural Sciences, Zhengzhou, China) for kindly providing the research materials, Dr. Xianchun Xia (Chinese Academy of Agricultural Sciences, Beijing, China) for donation some primers and Professor Weisheng Zhu (Suzhou Institute of Agricultural Sciences, Suzhou, China) for helping to plant the research materials and collect the phenotypic data in Suzhou.

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Copyright information

© Springer Science+Business Media B.V. 2008

Authors and Affiliations

  • Kunpu Zhang
    • 1
  • Jichun Tian
    • 1
  • Liang Zhao
    • 1
  • Bin Liu
    • 1
  • Guangfeng Chen
    • 2
  1. 1.State Key Laboratory of Crop BiologyGroup of Quality Wheat Breeding of Shandong Agricultural UniversityTaianChina
  2. 2.Department of AgriculturalDezhou CollegeDezhouChina

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