Genetica

, Volume 135, Issue 2, pp 245–255 | Cite as

Comparison of genomes of eight species of sections Linum and Adenolinum from the genus Linum based on chromosome banding, molecular markers and RAPD analysis

  • Olga V. Muravenko
  • Olga Yu. Yurkevich
  • Nadezhda L. Bolsheva
  • Tatiana E. Samatadze
  • Inna V. Nosova
  • Daria A. Zelenina
  • Alexander A. Volkov
  • Konstantin V. Popov
  • Alexander V. Zelenin
Article

Abstract

Karyotypes of species sects. Linum and Adenolinum have been studied using C/DAPI-banding, Ag-NOR staining, FISH with 5S and 26S rDNA and RAPD analysis. C/DAPI-banding patterns enabled identification of all homologous chromosome pairs in the studied karyotypes. The revealed high similarity between species L. grandiflorum (2n = 16) and L. decumbens by chromosome and molecular markers proved their close genome relationship and identified the chromosome number in L. decumbens as 2n = 16. The similarity found for C/DAPI-banding patterns between species with the same chromosome numbers corresponds with the results obtained by RAPD-analysis, showing clusterization of 16-, 18- and 30-chromosome species into three separate groups. 5S rDNA and 26S rDNA were co-localized in NOR-chromosome 1 in the genomes of all species investigated. In 30-chromosome species, there were three separate 5S rDNA sites in chromosomes 3, 8 and 13. In 16-chromosome species, a separate 5S rDNA site was also located in chromosome 3, whereas in 18-chromosome species it was found in the long arm of NOR-chromosome 1. Thus, the difference in localization of rDNA sites in species with 2n = 16, 2n = 30 and 2n = 18 confirms taxonomists opinion, who attributed these species to different sects. Linum and Adenolinum, respectively. The obtained results suggest that species with 2n = 16, 2n = 18 and 2n = 30 originated from a 16-chromosome ancestor.

Keywords

Ag-NOR-staining C/DAPI-banding FISH 5S and 26S rDNA Evolution Linum Chromosome identification RAPD-analysis Taxonomy 

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Copyright information

© Springer Science+Business Media B.V. 2008

Authors and Affiliations

  • Olga V. Muravenko
    • 1
  • Olga Yu. Yurkevich
    • 1
  • Nadezhda L. Bolsheva
    • 1
  • Tatiana E. Samatadze
    • 1
  • Inna V. Nosova
    • 1
  • Daria A. Zelenina
    • 2
  • Alexander A. Volkov
    • 2
  • Konstantin V. Popov
    • 1
  • Alexander V. Zelenin
    • 1
  1. 1.Engelhardt Institute of Molecular BiologyRussian Academy of SciencesMoscowRussia
  2. 2.Russian Federal Institute for Fisheries and OceanographyMoscowRussia

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