, Volume 135, Issue 2, pp 185–198 | Cite as

Genetic diversity and gene flow in a Caribbean tree Pterocarpus officinalis Jacq.: a study based on chloroplast and nuclear microsatellites

  • F. Muller
  • M. Voccia
  • A. Bâ
  • J. -M. Bouvet


We analysed the molecular diversity of Pterocarpus officinalis, a tree species distributed in Caribbean islands, South and Central America to quantify the genetic variation within island, to assess the pattern of differentiation and infer levels of gene flow; with the overall goal of defining a strategy of conservation. Two hundred two individuals of 9 populations were analysed using three chloroplast and six nuclear microsatellite markers. The observed heterozygosity varied markedly among the populations for nuclear (H Onuc = 0.20–0.50) and chloroplast microsatellites (H cp = 0.22–0.68). The continental population from French Guyana showed a higher value of H Onuc than island populations, and the differences were significant in some cases. The fixation index F IS ranged from −0.043 to 0.368; a significant heterozygote deficit was detected in 7 populations. The heterozygosity excess method suggested that two populations in Guadeloupe have undergone a recent bottleneck. Global and pairwise F ST were high for both nuclear (F STnuc = 0.29) and chloroplast microsatellites (F STcp = 0.58). The neighbour-joining tree based on both markers, presented a differentiation pattern that can be explained by the seed dispersal by flotation and marine stream. The comparison of Bayesian approach and the method based on allelic frequency demonstrate a very limited number of migrants between populations.


Pterocarpus officinalis Jacq. Nuclear microsatellites Chloroplast microsatellites Bayesian clustering Genetic structure Migrants 



We would like to thank the French Ministry MEDD (Ministère de L’Ecologie et du Développement Durable) for financial support in implementing the Pterocarpus officinalis project. This work was supported financially by the Guadeloupe Archipelago Region and the Social European Fund. We are very grateful to colleagues at CIRAD, C. Leroux, G. Bena, and at the University of Antilles-Guyane for assistance in collecting samples, and to A. Vaillant and M. Poitel at CIRAD for their technical assistance in the laboratory. We are grateful to the two anonymous reviewers for their helpful comments and suggestions which improve significantly the manuscript.


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Copyright information

© Springer Science+Business Media B.V. 2008

Authors and Affiliations

  • F. Muller
    • 1
    • 2
    • 3
  • M. Voccia
    • 1
  • A. Bâ
    • 2
  • J. -M. Bouvet
    • 1
  1. 1.Biological System Department, Research unit “genetic diversity and breeding of forest tree species”CIRADMontpellier cedex 5France
  2. 2.Laboratoire de Biologie et Physiologie Végétales, UFR des Sciences Exactes et NaturellesUniversité des Antilles et de la GuyanePointe-a-PitreFrance
  3. 3.Laboratoire des Symbioses Tropicales et MéditerranéennesUMR 1063 IRD/INRA/CIRAD/ENSA-M/UM2Montpellier cedex 5France

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