Genetica

, Volume 135, Issue 2, pp 169–183

A striking lack of genetic diversity across the wide-ranging amphibian Gastrophryne carolinensis (Anura: Microhylidae)

  • Robert Makowsky
  • Jason Chesser
  • Leslie J. Rissler
Article

DOI: 10.1007/s10709-008-9267-5

Cite this article as:
Makowsky, R., Chesser, J. & Rissler, L.J. Genetica (2009) 135: 169. doi:10.1007/s10709-008-9267-5

Abstract

We examine phylogeographic structure across a wide-ranging microhylid frog (Gastrophrynecarolinensis) using both mitochondrial (mtDNA) and nuclear (AFLP) data. Species with similar ecological characteristics such as large range size, low vagility, or existence across known biogeographic barriers, often are comprised of multiple, cryptic lineages. Surprisingly, our analyses of both portions of the genome show very little phylogeographic or population genetic structure. The family Microhylidae is one of the largest families of anurans with over 60 genera and around 400 species distributed across much of the world (Americas, Asia, Africa, and Madagascar), but very few phylogeographic studies have assessed intraspecific genetic diversity across the mitochondrial and nuclear genomes. Our results suggest that G. carolinensis, one of only three species of microhylid native to the US, has experienced a severe population bottleneck with subsequent range expansion. Comparable molecular data from closely related microhylids, in addition to demographic and ecological analyses, will provide valuable insight into patterns of genetic diversity and the processes driving phylogeographic diversity in these wide-ranging frogs.

Keywords

AFLP Gastrophryne Microhylidae mtDNA Phylogeography Southeastern U.S. 

Abbreviations

AFLP

Amplified fragment length polymorphism

ISSR

Intersimple sequence repeat

IUCN

The World Conservation Union

LC

Species of least concern

m

Meters

ML

Maximum-likelihood

mM

Millimolar

MP

Maximum parsimony

mtDNA

Mitochondrial DNA

ng

Nanograms

NJ

Neighbor-joining

μl

Microliter

PCoA

Principal coordinate analysis

RAPD

Random amplification of polymorphic DNA

cyt b

Cytochrome b

ND4

Nicotinamide adenine dinucleotide dehydrogenase subunit 4

tRNA

Transfer RNA

Copyright information

© Springer Science+Business Media B.V. 2008

Authors and Affiliations

  • Robert Makowsky
    • 1
    • 2
  • Jason Chesser
    • 1
    • 3
  • Leslie J. Rissler
    • 1
  1. 1.Department of Biological SciencesUniversity of AlabamaTuscaloosaUSA
  2. 2.Department of BiologyUniversity of Texas at ArlingtonArlingtonUSA
  3. 3.Department of BiologyUniversity of MississippiUniversityUSA

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