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Genetica

, Volume 135, Issue 2, pp 137–148 | Cite as

Somatic breakpoints, distribution of repetitive DNA and non-LTR retrotransposon insertion sites in the chromosomes of Chironomus piger Strenzke (Diptera, Chironomidae)

  • Paraskeva Michailova
  • Julia Ilkova
  • Tom Hankeln
  • Erwin R. Schmidt
  • Anna Selvaggi
  • Giampaolo Zampicinini
  • Gabriella SellaEmail author
Article

Abstract

Structural aberrations, their frequency and distribution as well as distribution of the tandem repetitive minisatellite DNA clusters of Alu and Hinf elements and two retroelements, the LINE NLRCth1 and the SINE CTRT1, were analyzed in the genome of the chironomid C. piger Strenzke larvae from a Bulgarian population. A consistent somatic variability in the structure of the polytene chromosomes was detected, showing that the C. piger genome is more actively rearranging than supposed before. Breakpoints were concentrated in proximal parts of chromosomes significantly more often than in distal parts. By FISH analysis we could detect only one locus containing Alu elements and 38 Hinf cluster loci which appear to be dispersed equally all over the chromosomes. The retrotransposons NLRCth1 and CTRT1 are present only in a few loci, but highly variant among different individuals. The mean number of NLRCth1 sites per individual was 18.4 ± 2.09 and of CTRT1 was 54.8 ± 8.42. A third of breakpoint locations were close to or coincide with a locus occupied by a retroelement (either NLRCth1 or CTRT1). Nineteen percent of breakpoints coincided with Hinf repetitive DNA elements. Some breakpoints were identical in the two sibling species C. piger and C. riparius Meigen (syn.: C. thummi thummi) and are considered as conserved hot spots of chromosome breakage.

Keywords

Chironomus Chromosomal aberrations Chromosomal breakages LINE Non-LTR retrotransposons Polytene chromosomes Minisatellite DNA SINE Tandem-repetitive DNA 

Abbreviations

BR

Balbiani ring

BSA

Bovine serum albumin

dNTP

Deoxy nucleotide triphosphate

dUTP

Deoxy uridine triphosphate

FISH

Fluorescence in situ hybridization

LINE

Long interspersed elements

NOR

Nucleolar organizer

ORF

Open reading frame

PCR

Polymerase chain reaction

SINE

Short interspersed elements

TE

Transposable element

TID

Transposon insertion display

Notes

Acknowledgments

This research was supported by a grant (FIRB) to Prof. G. Sella from the Italian Ministry of Education (MIUR), by a grant to Prof. P. Michailova from the Bulgarian Ministry of Education and Science (B1601) and a Collaborative Linkage NATO grant (LST CLG 980454) to Prof. G. Sella. The authors are indebted to Prof. C. Biémont for his valuable discussion of a former version of this paper and to two anonymous referees, whose criticisms greatly improved the manuscript.

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Copyright information

© Springer Science+Business Media B.V. 2008

Authors and Affiliations

  • Paraskeva Michailova
    • 1
  • Julia Ilkova
    • 1
  • Tom Hankeln
    • 2
  • Erwin R. Schmidt
    • 2
  • Anna Selvaggi
    • 3
  • Giampaolo Zampicinini
    • 3
  • Gabriella Sella
    • 3
    Email author
  1. 1.Institute of ZoologyBulgarian Academy of SciencesSofiaBulgaria
  2. 2.Institute of Molecular Genetics and Biosafety ResearchJohannes Gutenberg UniversityMainzGermany
  3. 3.Department of Animal BiologyUniversity of TurinTurinItaly

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