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Genetica

, 135:95 | Cite as

Microsatellite-based molecular diversity of bread wheat germplasm and association mapping of wheat resistance to the Russian wheat aphid

  • J. H. Peng
  • Y. Bai
  • S. D. Haley
  • N. L. V. Lapitan
Article

Abstract

Genetic diversity of a set of 71 wheat accessions, including 53 biotype 2 Russian wheat aphid (RWA2)-resistant landraces and 18 RWA2 susceptible accessions, was assessed by examining molecular variation at multiple microsatellite (SSR) loci. Fifty-one wheat SSR primer pairs were used, 81 SSR loci were determined, and 545 SSR alleles were detected. These SSR loci covered all the three genomes, 21 chromosomes, and at least 41 of the 42 chromosome arms. Diversity values averaged over SSR loci were high with mean number of SSR alleles/locus = 6.7, mean Shannon’s index (H) = 1.291, and mean Nei’s gene diversity (He) = 0.609. The three wheat genomes ranked as A > D > B and the homoeologous groups ranked as 7 > 3  > 1 > 2 > 6 > 5 > 4 based on the number of alleles per locus. Xgwm136 on chromosome arm 1AS is the most polymorphic SSR locus with the largest number of observed and effective alleles and the highest H and He. Among all 2485 pairs of wheat accessions, genetic distance (GD) ranged from 0.054 to 1.933 and averaged 0.9832. A dendrogram based on GD matrix showed that all the wheat accessions could be grouped into distinct clusters. Most of the susceptible cultivars (13/18) were clustered into groups that contains all or mostly susceptible accessions. Most of the U.S. cultivars belong to a group that is distinguishable from all the different RWA2 resistant groups. Diversity analysis was also conducted separately for subgroups containing 53 RWA2-resistant accessions and 18 RWA2-susceptible accessions. Association mapping revealed 28 SSR loci significantly associated with leaf chlorosis, and 8 with leaf rolling. New chromosome regions associated with RWA2 resistance were detected, and indicated existence of new RWA resistance genes located on chromosomes of all other homoeologous groups in addition to the groups 1 and 7 in bread wheat. This information is helpful for development of mapping populations for RWA2 resistance genes from different phylogenetic groups, and for wise utilization of the RWA-resistant germplasm in wheat breeding programs.

Keywords

Wheat germplasm Russian wheat aphid resistance Microsatellite Molecular diversity Genetic variation Association mapping 

Abbreviations

AM

Association mapping

LC

Leaf chlorosis

LD

Linkage disequilibrium

LR

Leaf rolling

GD

Genetic distance

PCR

Polymerase chain reaction

PIC

Polymorphic information content

RWA

Russian wheat aphid

SSR

Simple sequence repeat

UPGMA

The un-weighted pair-group method with arithmetic average

Notes

Acknowledgements

This study was supported in part by the Chinese Academy of Sciences under the Important Directional Program of Knowledge Innovation Engineering Grant No. KSCX2-YW-Z-0722, the Colorado Wheat Research Foundation, the U.S. Department of Agriculture under Cooperative Agreements USDA Contract No. 2001–52100-11293 and USDA Contract No. 2003-34205-13636, the National Research Initiative of USDA’s Cooperative State Research, Education and Extension Service, CAP Grant No. 2006-55606-16629, and Hatch Funds Project No. 644.

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Copyright information

© Springer Science+Business Media B.V. 2008

Authors and Affiliations

  • J. H. Peng
    • 1
    • 2
  • Y. Bai
    • 3
  • S. D. Haley
    • 2
  • N. L. V. Lapitan
    • 2
  1. 1.Wuhan Botanical Garden/InstituteChinese Academy of SciencesMoshan, WuhanChina
  2. 2.Department of Soil and Crop SciencesColorado State UniversityFort CollinsUSA
  3. 3.Department of Microbiology, Immunology and PathologyColorado State UniversityFort CollinsUSA

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