Advertisement

Genetica

, Volume 133, Issue 1, pp 77–84 | Cite as

DNA marker analysis for pyramided of Fusarium head blight (FHB) resistance QTLs from different germplasm

  • J. R. Shi
  • D. H. Xu
  • H. Y. Yang
  • Q. X. Lu
  • T. Ban
Article

Abstract

A pyramided FHB resistance line of wheat (WSY) was previously developed from three FHB resistant cultivars (Sumai 3, Wangshuibai, and Nobeokabouzu) in the Jiangsu Academy of Agricultural Sciences, China. In the present study, we analyzed the genetic relationship between WSY and the three parental cultivars using DNA markers in order to clarify how many and which resistance genes had accumulated in WSY. We analyzed 282 DNA markers from the 21 wheat chromosomes. WSY was found to include different chromosome regions that harbored putative FHB QTLs of the three parental germplasm. Haplotypes of DNA markers on these QTL regions revealed that the 1BL, 2BL, 5AS, and 7AL QTL regions were from Sumai 3, the 2AS, 2DS, 3AS, and 6BS QTL regions were from Wangshuibai, and the 3BS QTL region was from Nobeokabouzu. This study showed that different resistance genes from the different resistant germplasm had indeed accumulated in WSY. WSY is a potential resistant resource for FHB resistance in wheat breeding programs.

Keywords

Triticum aestivum Fusarium head blight DNA markers 

Notes

Acknowledgements

This research was partially funded by the Asia-Biotechnology fellowship program of the Japan International Research Center for Agricultural Sciences (JIRCAS), Japan and the Science foundation (BK2004171) and Talent foundation (2004, type A) projects of Jiangsu province, China.

References

  1. Anderson JA, Stack RW, Liu S, Waldron BL, Fjeld AD, Coyne C, Moreno-Sevilla B, Fetch JM, Song QJ, Cregan PB, Frohberg RC (2001) DNA markers for Fusarium head blight resistance QTLs in two wheat populations. Theor Appl Genet 102:1164–1168CrossRefGoogle Scholar
  2. Bai GH, Shaner G (2004) Management and resistance in wheat and barley to Fusarium head blight. Ann Rev Phytopathol 42:135–161CrossRefGoogle Scholar
  3. del Blanco I, Frohberg RC, Stack RW, Berzonsky WA, Kianian SF (2003) Detection of QTL linked to Fusarium head blight resistance in Sumai 3-derived North Dakota bread wheat lines. Theor Appl Genet 106:1027–1031PubMedGoogle Scholar
  4. Buerstmayr H, Lemmens M, Hartl L, Doldi L, Steiner B, Stierschneider M, Ruckenbauer P (2002) Molecular mapping of QTLs for Fusarium head blight resistance in spring wheat. I. Resistance to fungal spread (Type II resistance). Theor Appl Genet 104:84–91PubMedCrossRefGoogle Scholar
  5. Buerstmayr H, Steiner B, Hartl L, Griesser M, Angerer N, Lengauer D, Miedaner T, Schneider B, Lemmens M (2003) Molecular mapping of QTLs for Fusarium head blight resistance in spring wheat. II. Resistance to fungal penetration and spread. Theor Appl Genet 107:503–508PubMedCrossRefGoogle Scholar
  6. Edwards K, Johnstone C, Thompson C (1991) A simple and rapid method for the preparation of plant genomic DNA for PCR analysis. Nucleic Acids Res 19:1349PubMedCrossRefGoogle Scholar
  7. Felsenstein J (1995) PHYLIP (Phylogeny inference package) version 3.57c. Univ. Washington Press, SeattleGoogle Scholar
  8. Gonzalez-Hernandez JL, del Blanco A, Kianian SF, Ali S, Berzonsky WA, Kianian SF (2003) Wangshuibai: a hexaploid wheat resistant to the spread of Fusarium head bright. In: 2003 national Fusarium head bright forum proceedings. Bloomington, Minnesota, USA, December 13–15, pp 14–17Google Scholar
  9. Jia GF, Chen PD, Qin GJ, Bai GH, Wang X, Wang SL, Zhou B, Zhang SZ, Liu DJ (2006) QTLs for Fusarium head blight response in a wheat DH population of Wangshuibai/Alondra‘s’. Euphytica 146:183–191CrossRefGoogle Scholar
  10. Liao YC, Yu YJ (1985) Genetic analysis of scab resistance in the local wheat variety Wangshuibai. J Huazhong Agric College 45:6–14 (in Chinese with English abstract)Google Scholar
  11. Lin F, Kong ZX, Zhu HL, Xue SL, Wu JZ, Tian DG, Wei JB, Zhang CQ, Ma ZQ (2004) Mapping QTL associated with resistance to Fusarium head blight in the Nanda2419 × Wangshuibai population. I. Type II resistance. Theor Appl Genet 109:1504–1511PubMedCrossRefGoogle Scholar
  12. Lu WZ, Chen SH, Wang YZ (2001) Research on wheat scab. Science Publication House, Beijing, ChinaGoogle Scholar
  13. Mesterhazy A (1995) Types and components of resistance against FHB of wheat. Plant Breed 114:377–386CrossRefGoogle Scholar
  14. Minch E, Ruiz-Linares A, Goldstein D, Feldman M, Cavalli-Sforza LL (1997) Microsat (version <WHATEVER>): a computer program for calculating various statistics on microsatellite allele data. WWW: http://hpgl.stanford.edu/projects/microsat/Google Scholar
  15. Najaphy A, Chalmers KJ, Tourchi M, Mohammadi SA, Moghaddam M (2004) Mapping of quantitative trait loci for Fusarium head blight resistance in wheat. In: Proceedings of the 2nd international symposium on Fusarium head blight. Orlando, Florida, USA, December 11–15, pp 133–136Google Scholar
  16. Paillard S, Schnurbusch T, Winzeler M, Messmer M, Sourdille P, Abderhalden O, Keller B, Schachermayr G (2003) An integrative genetic linkage map of winter wheat (Triticum aestivum L.). Theor Appl Genet 107:1235–1242PubMedCrossRefGoogle Scholar
  17. Pestsova E, Ganal MW, Roder MS (2000) Isolation and mapping of microsatellite markers specific for the D genome of bread wheat. Genome 43:689–697PubMedCrossRefGoogle Scholar
  18. Roder MS, Korzun V, Wendehake K, Plaschke J, Tixier MH, Leroy P, Ganal MW (1998) A microsatellite map of wheat. Genetics 149:2007–2023PubMedGoogle Scholar
  19. Schroeder HW, Christensen JJ (1963) Factors affecting resistance of wheat to scab caused by Gibberella zeae. Phytopathology 53:831–838Google Scholar
  20. Shi JR, Song QJ, Singh S, Lewis J, Ward RW, Cregan P, Gill BS (2003) Genetic and physical maps of Xbarc SSR loci in wheat. In: 2003 national Fusarium head bright forum proceedings. Bloomington, Minnesota, USA, December 13–15, pp 14–17Google Scholar
  21. Somers DJ, Isaac P, Edwards K (2004) A high-density microsatellite consensus map for bread wheat (Triticum aestivum L.). Theor Appl Genet 109:1105–1114PubMedCrossRefGoogle Scholar
  22. Song QJ, Shi JR, Singh S, Fickus EW, Costa JM, Lewis J, Gill BS, Ward R, Cregan PB (2005) Development and mapping of microsatellite (SSR) markers in wheat. Theor Appl Genet 110:550–560PubMedCrossRefGoogle Scholar
  23. Waldron BL, Moreno-Sevilla B, Anderson JA, Stack RW, Frohberg RC (1999) RFLP mapping of QTL for Fusarium head blight resistance in wheat. Crop Sci 39:805–811CrossRefGoogle Scholar
  24. Windels CE (2000) Economic and social impacts of Fusarium head blight: Changing farms and rural communities in the Northern Great Plains. Phytopathology 90:17–21CrossRefPubMedGoogle Scholar
  25. Xu DH, Juan HF, Nohda M, Ban T (2001) QTLs Mapping of type I and type II Resistance to FHB in Wheat. In: 2001 national Fusarium head blight forum proceedings. Erlanger, Kentucky, USA, December 8–10, pp 40–42Google Scholar
  26. Xu DH, Ban T (2004) Conversion of AFLP markers associated with FHB resistance in wheat into STS markers with an extension-AFLP method. Genome 47:660–665PubMedCrossRefGoogle Scholar
  27. Yang ZP, Gilbert J, Fedak G, Somers DJ (2005) Genetic characterization of QTL associated with resistance to Fusarium head blight in a doubled-haploid spring wheat population. Genome 48:187–196PubMedGoogle Scholar
  28. Zhang X, Zhou MP, Ren LJ, Bai GH, Ma HX, Scholten OE, Guo PG, Lu WZ (2004) Molecular characterization of Fusarium head blight resistance from wheat variety Wangshuibai. Euphytica 139:59–64CrossRefGoogle Scholar
  29. Zhou WC, Kolb FL, Bai GH, Shaner G, Domier LL (2002) Genetic analysis of scab resistance QTL in wheat with microsatellite and AFLP markers. Genome 45:719–727PubMedCrossRefGoogle Scholar
  30. Zhou WC, Kolb FL, Yu JB, Bai GH, Boze LK, Domier LL (2004) Molecular characterization of Fusarium head blight resistance in Wangshuibai with simple sequence repeat and amplified fragment length polymorphism markers. Genome 47:1137–1143PubMedCrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media B.V. 2007

Authors and Affiliations

  • J. R. Shi
    • 1
  • D. H. Xu
    • 2
  • H. Y. Yang
    • 1
  • Q. X. Lu
    • 1
  • T. Ban
    • 2
  1. 1.Jiangsu Academy of Agricultural SciencesNanjingChina
  2. 2.Biological Resources DivisionJapan International Research Center for Agricultural Sciences (JIRCAS)TsukubaJapan

Personalised recommendations