Genetica

, Volume 132, Issue 3, pp 281–286 | Cite as

Comparison of karyotypes of Acipenser oxyrinchus and A. sturio by chromosome banding and fluorescent in situ hybridization

  • Francesco Fontana
  • Massimo Lanfredi
  • Frank Kirschbaum
  • Manuel A. Garrido-Ramos
  • Francisca Robles
  • Anna Forlani
  • Leonardo Congiu
Article

Abstract

A highly debated problem in Acipenseridae taxonomy is whether Acipenser oxyrinchus (North American Atlantic sturgeon) and A. sturio (European Atlantic sturgeon) are true species: a detailed comparison of their karyotypes could provide relevant information. Here we describe for the first time the karyotype of A. oxyrinchus (2n = 121 ± 3), and its features, among which the constitutive heterochromatin, revealed by C-banding technique, the distribution of telomeric regions, and the 5S rRNA genes, detected by FISH. The results reveal that A. oxyrinchus and A. sturio karyotypes and features are quite similar. Moreover, comparing the results obtained through hybridization by FISH with HindIII and PstI satellite DNA in these and in other sturgeon species, no hybridization signals are detected in A. sturio and A. oxyrinchus, while A. stellatus and A. gueldenstaedtii show hybridization. Thus A. sturio and A. oxyrinchus appear very similar from a cytogenetic point of view: these and molecular data repeatedly point out that A. sturio and A. oxyrinchus represent a sister clade in comparison to all other sturgeon species up to now studied.

Keywords

Atlantic sturgeon Karyotype C-banding Telomeric sequence Satellite DNA 5S rDNA 

References

  1. Artuykhin E, Vecsei P (1999) On the status of Atlantic sturgeon: conspecificity of European Acipenser sturio and the North American Acipenser oxyrhynchus. J Appl Ichthyol (Special Issue) 15(4–5):35–37CrossRefGoogle Scholar
  2. Birstein VJ, Bemis WE (1997) How many species are there within the genus Acipenser? Environ Biol Fish 48:157–163CrossRefGoogle Scholar
  3. Birstein VJ, DeSalle R (1998) Molecular phylogeny of Acipenserinae. Mol Phylogenet Evol 9:141–155PubMedCrossRefGoogle Scholar
  4. Birstein VJ, Doukakis P (2000) Molecular analysis of Acipenser sturio, L., 1758 and A. oxyrinchus Mitchill, 1815: A review. Bol Inst Esp Oceanogr 16:61–73Google Scholar
  5. Chicca M, Suciu R, Ene C, Lanfredi M, Congiu L, Leis M, Tagliavini J, Rossi R, Fontana F (2002) Karyotype characterization of the stellate sturgeon, Acipenser stellatus, by chromosome banding and fluorescent in situ hybridization. J Appl Ichthyol 18:298–300CrossRefGoogle Scholar
  6. Choudhury A, Dick TA (1998) The historical biogeography of sturgeons (Osteichthyes : Acipenseridae): a synthesis of phylogenetics, palaeontology and palaeogeography. J Biogeog 25:623– 640CrossRefGoogle Scholar
  7. De La Hérran R, Ruiz Rejòn C, Ruiz Rejòn M, Garrido-Ramos MA (2001) The molecular phylogeny of the Sparidae (Pisces, Perciformes) based on two satellite DNA families. Heredity 87:691–697PubMedCrossRefGoogle Scholar
  8. Fontana F (2002) A cytogenetic approach to the study of taxonomy and evolution in sturgeons. J Appl Ichthyol 18:226–233CrossRefGoogle Scholar
  9. Fontana F, Colombo G (1974) The chromosomes of italian sturgeons. Experientia 30:739–742PubMedCrossRefGoogle Scholar
  10. Fontana F, Lanfredi M, Rossi R, Bronzi P, Arlati G (1995) Etablished cell lines from three sturgeon species. Sturgeon Quarterly 3(4):6–7Google Scholar
  11. Fontana F, Tagliavini J, Congiu L, Lanfredi M, Chicca M, Laurenti C, Rossi R (1998) Karyotypic characterization of the great sturgeon, Huso huso, by multiple staining techniques and fluorescent in situ hybridization. Mar Biol 132:495–501CrossRefGoogle Scholar
  12. Fontana F, Tagliavini J, Congiu L (2001) Sturgeon genetics and cytogenetics: recent advancements and perspectives. Genetica 111:359–373PubMedCrossRefGoogle Scholar
  13. Gardiner BG (1984) Sturgeons as living fossils. In: Eldredge N, Stanley SM (eds) Living Fossils. Springer-Verlag, New York, pp 148–152Google Scholar
  14. Garrido-Ramos MA, Soriguer MC, De la Herrán R, Jamilena M, Ruiz Rejón C, Domezain A, Hernando JA, Ruiz Rejón M (1997) Morphometric and genetic analysis as proof of the existence of two sturgeon species in the Guadalquivir river. Mar Biol 129:33–39CrossRefGoogle Scholar
  15. Krieger J, Fuerst PA (2002) Evidence for a slowed rate of molecular evolution in the Order Acipenseriformes. Mol Biol Evol 19:891–897PubMedGoogle Scholar
  16. Lanfredi M, Congiu L, Garrido-Ramos MA, De La Herrán R, Leis M, Chicca M, Rossi R, Tagliavini J, Ruiz Rejón C, Ruiz Rejón M, Fontana F (2001) Chromosomal location and evolution of a satellite DNA family in seven sturgeon species. Chrom Res 9:47–52PubMedCrossRefGoogle Scholar
  17. Li MF, Marrayatt V, Annand C, Odense P (1985) Fish cell culture: two newly developed cell lines from Atlantic sturgeon (Acipenser oxyrhynchus) and guppy (Poecilia reticulata). Can J Zool 63:2867–2874CrossRefGoogle Scholar
  18. Ludwig A, May B, Debus L, Jennekens I (2000) Heteroplasmy in the mtDNA control region of sturgeon (Acipenser, Huso and Scaphirhynchus). Genetics 156:1933–1947PubMedGoogle Scholar
  19. Ludwig A, Belfiore NM, Pitra C, Svirsky V, Jenneckens I (2001) Genome duplication events and functional reduction of ploidy levels in sturgeon (Acipenser, Huso and Scaphirhynchus). Genetics 158:1203–1215PubMedGoogle Scholar
  20. Ludwig A, Debus I, Lieckfeldt D, Wirgin I, Benecke N, Jenneckens I, Williot P, Waldman JR, Pitra C (2002) When the American sea sturgeon swam east. Nature 419:447–448PubMedCrossRefGoogle Scholar
  21. Magnin E, Beaulieau G (1963) Étude morphométrique comparée de l’Acipenser oxyrhynchus Mitchill du Saint-Laurent et l’Acipenser sturio Linné de la Gironde. Natur Can 90:5–38Google Scholar
  22. Ong TL, Stabile J, Wirgin I, Waldman JR (1996) Genetic divergence between Acipenser oxyrinchus oxyrinchus and A. o. desotoi as assessed by mitochondrial DNA sequencing analysis. Copeia 1996:464–469CrossRefGoogle Scholar
  23. Robles F, De La Herrán R, Ludwig A, Ruiz Rejón C, Ruiz Rejón M, Garrido-Ramos MA (2004) Evolution of ancient satellite DNAs in sturgeon genomes. Gene 338:133–142PubMedCrossRefGoogle Scholar
  24. Robles F, De La Herrán R, Ludwig A, Ruiz Rejón C, Ruiz Rejón M, Garrido-Ramos MA (2005) Genomic organization and evolution of the 5S ribosomal DNA in the ancient fish sturgeon. Genome 48:18–28PubMedCrossRefGoogle Scholar
  25. Smith HM (1891) Report on the fisheries of the South Atlantic States. Bull U S Fish Comm 11:269–356Google Scholar
  26. Tagliavini J, Williot P, Congiu L, Chicca M, Lanfredi M, Rossi R, Fontana F (1999) Molecular cytogenetic analysis of the karyotype of the European Atlantic sturgeon, Acipenser sturio. Heredity 83:520–525PubMedCrossRefGoogle Scholar
  27. Tiedemann R, Moll K, Paulus KB, Scheer M, Williot P, Bartel R, Gessner J, Kirschbaum F (2006) Atlantic sturgeons (Acipenser sturio, Acipenser oxyrinchus): American females successful in Europe. Naturwissenschaften DOI 10.1007/s00114–006–0175-1 Google Scholar
  28. Vladykov VD (1955) A comparison of Atlantic sea sturgeon with a new subspecies from the Gulf of Mexico (Acipenser oxyrhynchus desotoi). J Fish Res Board Can 12:754–761Google Scholar
  29. Wirgin II, Stabile JE, Waldman JR (1997) Molecular analysis in the conservation of sturgeons and paddlefish. Environ Biol Fish 48:385–398CrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media B.V. 2007

Authors and Affiliations

  • Francesco Fontana
    • 1
  • Massimo Lanfredi
    • 1
  • Frank Kirschbaum
    • 2
  • Manuel A. Garrido-Ramos
    • 3
  • Francisca Robles
    • 3
  • Anna Forlani
    • 1
  • Leonardo Congiu
    • 1
  1. 1.Department of BiologyUniversity of FerraraFerraraItaly
  2. 2.Leibniz-Institute of Freshwater Ecology and Inland FisheriesBerlinGermany
  3. 3.Departamento de Genetica, Facultad de CienciasUniversidad de GranadaGranadaSpain

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