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Genetica

, Volume 132, Issue 2, pp 123–129 | Cite as

Genetic variation in the endangered Anisodus tanguticus (Solanaceae), an alpine perennial endemic to the Qinghai-Tibetan Plateau

  • Wei Zheng
  • Liuyang Wang
  • Lihua Meng
  • Jianquan Liu
Article

Abstract

We used random amplified polymorphic DNA markers (RAPDs) to assess genetic variation between- and within-populations of Anisodus tanguticus (Solanaceae), an endangered perennial endemic to the Qinghai-Tibetan Plateau with important medicinal value. We recorded a total of 92 amplified bands, using 12 RAPD primers, 76 of which (P = 82.61%) were polymorphic, and calculated values of Ht and Hsp of 0.3015 and 0.4459, respectively, suggesting a remarkably high rate of genetic variation at the species level. The average within-population diversity also appeared to be high, with P, He and Hpop values of 55.11%, 0.1948 and 0.2918, respectively. Analyses of molecular variance (AMOVA) showed that among- and between-population genetic variation accounted for 67.02% and 32.98% of the total genetic variation, respectively. In addition, Nei’s coefficient of differentiation (GST) was found to be high (0.35), confirming the relatively high level of genetic differentiation among the populations. These differentiation coefficients are higher than mean corresponding coefficients for outbreeding species, but lower than reported coefficients for some rare species from this region. The genetic structure of A. tanguticus has probably been shaped by its breeding attributes, biogeographic history and human impact due to collection for medicinal purposes. The observed genetic variations suggest that as many populations as possible should be considered in any planned in situ or ex situ conservation programs for this species.

Keywords

Anisodustanguticus RAPDs Genetic diversity Conservation The Qiughai-Tibetan Plateau 

Notes

Acknowledgements

This work was supported by grants from the Chinese Academy of Sciences (Key Innovation Plan KSCX2-SW-106 and the Special Fund for Outstanding PhD Dissertation).

References

  1. Bolaric S, Barth S, Melchinger AE, Posselt UK (2005) Genetic diversity in European perennial ryegrass cultivars investigated with RAPD markers. Plant Breed 124:161–166CrossRefGoogle Scholar
  2. Borowsky R (2001) Estimating nucleotide diversity from random amplified polymorphic DNA and amplified fragment length polymorphism data. Mol Phylogenet Evol 18:143–148PubMedCrossRefGoogle Scholar
  3. Bussell JD (1999) The distribution of random amplified polymorphic DNA (RAPD) diversity amongst populations of Isotoma petraea (Lobeliaceae). Mol Ecol 8:775–789CrossRefGoogle Scholar
  4. Carlson JE (1991) Segregation of random amplified DNA Markers in F1 progeny of conifers. Theor Appl Genet 83:194–200CrossRefGoogle Scholar
  5. Chen SL, Xia T, Chen SY, Zhou YJ (2005) RAPD Profiling in detecting genetic variation in endemic Coelonema (Brassicaceae) of Qinghai-Tibet Plateau of China. Biochem Genet 43:189–201PubMedCrossRefGoogle Scholar
  6. Doyle JJ, Doyle JL (1987) A rapid DNA isolation procedure for small quantities of fresh leaf material. Phytochem Bull 19:11–15Google Scholar
  7. Ellstand NC, Elam DR (1993) Population genetic consequences of small population size: implications for plant conservation. Annu Rev Ecol Syst 24:217–242CrossRefGoogle Scholar
  8. Ennos RA (1994) Estimating the relative rates of pollen and seed migration among plant populations. Heredity 72:250–259Google Scholar
  9. Excoffier L, Smouse PE, Quattro JM (1992) Analysis of molecular variance inferred from metric distance among DNA haplotypes: application to human mitochondrial DNA restriction data. Genetics 131:176–191Google Scholar
  10. Gabrielsen TM, Bachmann K, Jakobsen KS, Brochmann C (1997) Glacial survival does not matter: RAPD phylogeography of Nordic Saxifraga oppositifolia. Mol Ecol 6:831–842CrossRefGoogle Scholar
  11. Ge XJ, Zhang LB, Yuan YM, Hao G, Chiang TY (2005) Strong genetic differentiation of the East-Himalayan Megacodon stylophorus (Gentianaceae) detected by inter-simple sequence repeats (ISSR). Biodivers Conserv 14:849–861CrossRefGoogle Scholar
  12. Hamrick JL, Godt MJW (1989) Allozyme diversity in plant species. In: Brown AHD, Clegg MT, Kahler AL, Weir BS (eds) Population genetics and germplasm resources in crop improvement. Sinauer Associates, SunderlandGoogle Scholar
  13. Hamrick JL, Godt MJW (1996) Conservation genetics of endemic plant species. In: Avise JC, Hamrick JL (eds) Conservation genetics: case histories from nature. Chapman & Hall, New YorkGoogle Scholar
  14. He YJ, Cui GF, Feng ZW, Zheng J, Dong JS, Li YB (2004) Conservation priorities for plant species of forest-meadow ecotone in Sanjiangyuan nature reserve. Chin J Appl Ecol 15(8):1307–1312Google Scholar
  15. Huff DR, Peakall R, Smouse PE (1993) RAPD variation within and among natural populations of outcrossing buffalograss (Buchloe dactyloides (Nutt.) Engelm). Theor Appl Genet 8:927–934Google Scholar
  16. Liu JQ, Gao TG, Chen ZD, Lu AM (2002) Molecular phylogeny and biogeography of the Qinghai-Tibet Plateau endemic Nannoglottis (Asteraceae). Mol Phylogenet Evol 23:307–325PubMedCrossRefGoogle Scholar
  17. Liu JQ, Wang YJ, Wang AL, Ohba H, Abbott R (2006a) Radiation and diversification of Ligularia-Cremanthodium-Parasenecio triggered by uplifts of the Qinghai-Tibetan Plateau. Mol Phylogenet Evol 38:31–49PubMedCrossRefGoogle Scholar
  18. Liu JM, Wang L, Geng YP, Wang QB, Luo LJ, Zhong Y (2006b) Genetic diversity and population structure of Lamiophlomis rotata (Lamiaceae), an endemic species of Qinghai–Tibet Plateau. Genetica 128:385–394PubMedCrossRefGoogle Scholar
  19. Lynch M, Milligan GG (1994) Analysis of population genetic structure with RAPD markers. Mol Ecol 3:91–99PubMedGoogle Scholar
  20. Miller MP (1997) Tools for population genetic analysis. Version 1.3. Department of Biological Sciences, Northern Arizona University, FlagstaffGoogle Scholar
  21. Miller MP (1998) Amova-Prep 1.01. A program for the preparation of AMOVA input files from dominant-market raw data. Northern Arizona University, Flagstaff AZ. http:// herb.bio.nau.edu/miller/amovaprp.htmGoogle Scholar
  22. Myers N, Mittermeier RA, Mittermeier CG, Fonseca da GAB, Kentm J (2000) Biodiversity hotspots for conservation priorities. Nature 403:853–858PubMedCrossRefGoogle Scholar
  23. Nei M (1978) Estimation of average heterozygosity and genetic distance from a small number of individuals. Genetics 89:583–590PubMedGoogle Scholar
  24. Newton AC, Allnutt TR, Gillies ACM, Lowe AJ, Ennos RA (1999) Molecular phylogeography, intraspecific variation and the conservation of tree species. Trends Ecol Evol 14:140–145PubMedCrossRefGoogle Scholar
  25. Nybom H (2004) Comparison of different nuclear DNA markers for estimating intraspecific genetic diversity in plants. Mol Ecol 13:1143–1155PubMedCrossRefGoogle Scholar
  26. Peakall R, Smouse PE, Huff DR (1995) Evolutionary implications of allozyme and RAPD variation in diploid populations of dioecious buffalograss, Buchloe dactyloides. Mol Ecol 4:135–147Google Scholar
  27. Sambrook J, Fritsch EF, Maniatis T (eds) (1989) Molecular cloning: a laboratory manual. Cold Spring Harbor Press, Cold Spring Harbor, NYGoogle Scholar
  28. Schaal BA, Hayworth DA, Olsen KM, Rauscher JT, Smith WA (1998) Phylogeographic studies in plants: problems and prospects. Mol Ecol 7:465–474CrossRefGoogle Scholar
  29. Wang AL, Yang MH, Liu JQ (2005) Molecular phylogeny, recent radiation and evolution of gross morphology of the rhubarb genus Rheum (Polygonaceae) inferred from chloroplast DNA trnL-F sequences. Ann Bot London 96:489–498CrossRefGoogle Scholar
  30. Weising K, Nybom H, Wolff K, Meyer W (eds) (1995) DNA fingerprinting in plants and fungi. CRC Press, Boca RatonGoogle Scholar
  31. Wolfe AD, Liston A (1998) Contributions of PCR-based methods to plant systematics and evolutionary biology. In: Soltis DE, Soltis PS, Doyle JJ (eds) Molecular systematics of plants II, DNA sequencing. Kluwer Academic Publishers, DordrechtGoogle Scholar
  32. Xia T, Chen SL, Chen SY, Ge XJ (2005) Genetic variation within and among populations of Rhodiola alsia (Crassulaceae) native to the Tibetan Plateau as detected by ISSR markers. Biochem Genet 43:87–101PubMedCrossRefGoogle Scholar
  33. Yang DZ, Zhang ZY, Lu AM, Sun K, Liu JQ (2002) Floral organogenesis and development of two taxa of the Solanaceae-Anisodus tanguticus and Atropa belladonna. Israel J Plant Sci 50:127–134CrossRefGoogle Scholar
  34. Yang YC (ed) (1991) Tibetan medicines. Qinghai People Press, QinghaiGoogle Scholar
  35. Yap IV, Nelson RJ (1996) WinBoot: a program for performing bootstrap analysis of binary data to determine the confidence limits of UPGMA-based dendrograms. International Rice Research Instititute (IRRI), Manila, PhilippinesGoogle Scholar
  36. Yeh FC, Yang R, Boyle T (1999) POPGENE. Microsoft Windows-based freeware for population genetic analysis. Release 1.31. University of Alberta, Edmonton, CanadaGoogle Scholar
  37. Zheng D (1996) The system of physico-geographical regions of the Qinghai-Tibet (Xizang) Plateau. Sci China Ser D 39:410–417Google Scholar

Copyright information

© Springer Science+Business Media B.V. 2007

Authors and Affiliations

  • Wei Zheng
    • 1
    • 3
  • Liuyang Wang
    • 1
  • Lihua Meng
    • 1
  • Jianquan Liu
    • 1
    • 2
  1. 1.Key Laboratory of the Qinghai-Tibetan Plateau Ecosystem and Biological Evolution and Adaptation, Northwest Institute of Plateau BiologyThe Chinese Academy of SciencesXiningP.R. China
  2. 2.Key Laboratory of Arid and Grassland EcologyLanzhou UniversityLanzhouP.R. China
  3. 3.Graduate School of the Chinese Academy of SciencesBeijingP.R. China

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