, Volume 132, Issue 2, pp 123–129 | Cite as

Genetic variation in the endangered Anisodus tanguticus (Solanaceae), an alpine perennial endemic to the Qinghai-Tibetan Plateau

  • Wei Zheng
  • Liuyang Wang
  • Lihua Meng
  • Jianquan Liu


We used random amplified polymorphic DNA markers (RAPDs) to assess genetic variation between- and within-populations of Anisodus tanguticus (Solanaceae), an endangered perennial endemic to the Qinghai-Tibetan Plateau with important medicinal value. We recorded a total of 92 amplified bands, using 12 RAPD primers, 76 of which (P = 82.61%) were polymorphic, and calculated values of Ht and Hsp of 0.3015 and 0.4459, respectively, suggesting a remarkably high rate of genetic variation at the species level. The average within-population diversity also appeared to be high, with P, He and Hpop values of 55.11%, 0.1948 and 0.2918, respectively. Analyses of molecular variance (AMOVA) showed that among- and between-population genetic variation accounted for 67.02% and 32.98% of the total genetic variation, respectively. In addition, Nei’s coefficient of differentiation (GST) was found to be high (0.35), confirming the relatively high level of genetic differentiation among the populations. These differentiation coefficients are higher than mean corresponding coefficients for outbreeding species, but lower than reported coefficients for some rare species from this region. The genetic structure of A. tanguticus has probably been shaped by its breeding attributes, biogeographic history and human impact due to collection for medicinal purposes. The observed genetic variations suggest that as many populations as possible should be considered in any planned in situ or ex situ conservation programs for this species.


Anisodustanguticus RAPDs Genetic diversity Conservation The Qiughai-Tibetan Plateau 



This work was supported by grants from the Chinese Academy of Sciences (Key Innovation Plan KSCX2-SW-106 and the Special Fund for Outstanding PhD Dissertation).


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Copyright information

© Springer Science+Business Media B.V. 2007

Authors and Affiliations

  • Wei Zheng
    • 1
    • 3
  • Liuyang Wang
    • 1
  • Lihua Meng
    • 1
  • Jianquan Liu
    • 1
    • 2
  1. 1.Key Laboratory of the Qinghai-Tibetan Plateau Ecosystem and Biological Evolution and Adaptation, Northwest Institute of Plateau BiologyThe Chinese Academy of SciencesXiningP.R. China
  2. 2.Key Laboratory of Arid and Grassland EcologyLanzhou UniversityLanzhouP.R. China
  3. 3.Graduate School of the Chinese Academy of SciencesBeijingP.R. China

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