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Genetica

, Volume 128, Issue 1–3, pp 455–469 | Cite as

Repetitive DNA sequences include retrotransposons in genomes of the Glomeromycota

  • Armelle Gollotte
  • Floriane L’Haridon
  • Odile Chatagnier
  • Guillaume Wettstein
  • Christine Arnould
  • Diederik van Tuinen
  • Vivienne Gianinazzi-Pearson
Research Article

Abstract

Twenty-five repetitive elements are first described in the genomes of the arbuscular mycorrhizal (AM) fungi Gigaspora margarita, Gig. rosea and Glomus mosseae. Nineteen repetitive DNA sequences isolated by genomic library screening and four by self-priming PCR had no homology to known DNA sequences, except for two Gig. margarita sequences and one Gig. rosea sequence which showed amino acid similarity to retrotransposons. Part of the Gig. rosea sequence was also similar to a DNA transposon. Two other retrotransposon sequences were isolated using PCR targeting of reverse transcriptase and ribonuclease H domains. Evidence is provided for three gypsy-like LTR retrotransposon and two non-LTR retrotransposon sequences in the AM fungal genomes. Four contain stop codons indicating that they cannot be active. Expression of three retrotransposons was not detected in germinating spores or intraradical hyphae of Gig. margarita. Southern blot analyses indicated that these three sequences are dispersed in the genome and that two are methylated. Sequence analysis of different GmarLTR1 copies showed they have undergone mutations by transitions, which may have been induced by cytosine methylation. Transposable elements may have played a major role in shaping genome structure and size during evolution of the Glomeromycota.

Keywords

Arbuscular mycorrhizal fungi Cytosine methylation Gigaspora margarita Gigaspora rosea Glomus mosseae Repetitive DNA sequences Retrotransposons DNA transposons 

Abbreviations

AM fungus

Arbuscular mycorrhizal fungus

EDTA

Ethylenediaminetetraacetic acid

EN

Endonuclease

GSP-PCR

Genomic self-priming polymerase chain reaction

INT

Integrase

LSU

Large subunit

LTR

Long terminal repeat

M-MLV

Moloney murine leukemia virus

rDNA

Ribosomal DNA

RNase H

Ribonuclease H

RT

Reverse transcriptase

SDS

Sodium dodecyl sulfate

SSC

Sodium salt citrate

SSU

Small subunit

TAE

Tris/acetic acid/EDTA

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Copyright information

© Springer 2006

Authors and Affiliations

  • Armelle Gollotte
    • 1
  • Floriane L’Haridon
    • 1
  • Odile Chatagnier
    • 1
  • Guillaume Wettstein
    • 1
  • Christine Arnould
    • 1
  • Diederik van Tuinen
    • 1
  • Vivienne Gianinazzi-Pearson
    • 1
  1. 1.UMR 1088 INRA/5184 CNRS/Université de Bourgogne Plante-Microbe-EnvironnementINRA-CMSEDijon CedexFrance

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