Genetica

, Volume 125, Issue 2–3, pp 231–241

Microsatellite Analysis of Olive Fly Populations in the Mediterranean Indicates a Westward Expansion of the Species

  • A.A. Augustinos
  • Z. Mamuris
  • E.E. Stratikopoulos
  • S. D’Amelio
  • A. Zacharopoulou
  • K.D. Mathiopoulos
Article

Abstract

Bactrocera oleae is the major insect pest of the olive fruit. Twelve microsatellite loci isolated from the genome of this insect were used in a Mediterranean-wide population analysis. These loci were highly polymorphic with a mean number of alleles per locus of 10.42 and a mean effective number of alleles of 2.76. The analysis was performed on a sample of 671 flies collected from nineteen locations around the European part of the Mediterranean basin. Despite the high level of gene flow across the Mediterranean, results support the notion of a differentiation of three subpopulations: one of the Iberian Peninsula, one of Greece and Italy and one of Cyprus. In addition, the gradual decrease of heterozygosity from the Eastern to the Western part of the Mediterranean indicates a westward expansion of the species.

Key words:

Bactrocera (Dacus) oleae olive fly Tephritidae microsatellite polymorphism population structure colonization 

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References

  1. Allendorf, F.W., Phelps, S.R. 1981Use of allelic frequencies to describe population structureCan. J. Fish. Aquat. Sci.3815071514Google Scholar
  2. Ashburner, M. 1989Drosophila: A Laboratory ManualCold Spring Harbour Laboratory PressNew YorkGoogle Scholar
  3. Augustinos, A.A., Stratikopoulos, E.E., Zacharopoulou, A., Mathiopoulos, K.D. 2002Polymorphic microsatellite markers in the olive fly, Bactrocera oleaeMol. Ecol. Notes2278280CrossRefGoogle Scholar
  4. Bonizzoni, M., Malacrida, A.R., Guglielmino, C.R., Gomulski, L.M., Gasperi, G., Zheng, L. 2000Microsatellite polymorphism in the Mediterranean fruit fly, Ceratitis capitataInsect Mol. Biol.9251261CrossRefPubMedGoogle Scholar
  5. Bonizzoni, M., Katsoyannos, B.I., Marguerie, R., Guglielmino, C.R., Gasperi, G., Malacrida, A.R., C.hapman, T. 2002Microsatellite analysis reveals remating by wild Mediterranean fruit fly females Ceratitis capitataMol. Ecol.1119151921CrossRefPubMedGoogle Scholar
  6. Bonizzoni, M., Guglielmino, C.R., Smallridge, C.J., Gomulski, L.M., Malacrida, A.R., Gasperi, G. 2004On the origins of medfly invasion and expansion in AustraliaMol. Ecol.1338453855CrossRefPubMedGoogle Scholar
  7. Bush, G.L. & G.B. Kitto, 1979. Research on the genetic structure of wild and laboratory strains of the olive fly, pp. 10–20 in FAO Report. Development of Pest Management Systems for Olive Culture Program. Rome: FAO.GRE69/525Google Scholar
  8. Chakraborty, R., Nei, M. 1977Bottleneck effects on average heterozygosity and genetic distance with the stepwise mutation modelEvolution31347356Google Scholar
  9. Dover, G. 1995Slippery DNA runs on and on and on⋖Nat. Genet.10254256CrossRefPubMedGoogle Scholar
  10. Excoffier, L., Smouse, P.E., Quattro, J.M. 1992Analysis of Molecular Variance inferred from metric distances among DNA haplotypes – Application to human mitochondrial DNA restriction dataGenetics131479491PubMedGoogle Scholar
  11. FAOSTAT Agricultural Data, http://apps.fao.org/default/hpmGoogle Scholar
  12. Felsenstein, J. 1994 PHYLIP (Phylogeny Inference Package) version 3.6. Distributed by the AuthorDepartment of Genome Sciences, University of WashingtonSeattleGoogle Scholar
  13. Gasperi, G., Bonizzoni, M., Gomulski, L.M., Murelli, V., Torti, C., Malacrida, A.R., Guglielmino, C.R. 2002Genetic differentiation, gene flow and the origin of infestation of the medfly, Ceratitis capitataGenetica116125135CrossRefPubMedGoogle Scholar
  14. Harpending, H.C. & R.H. Ward, 1982. Chemical systematics and human populations, pp. 213–256 in Biochemical aspects of evolutionary biology, edited by M.H. Nitecki, University of Chicago PressGoogle Scholar
  15. Irvin, S.D., Wetterstrand, K.A., Hutter, C.M., Aquadro, C.F. 1998Genetic variation and differentiation at microsatellite loci in Drosophila simulans: evidence for founder effects in new world populationsGenetics1507797790Google Scholar
  16. Katsoyannos, P. 1992Olive Pests and Their Control in the Near East. FAO Plant Production and Protection Paper No115FAORomeGoogle Scholar
  17. Kinnear, M.W., Barriana, H.S., Sved, J.A., Frommer, M. 1998Polymorphic microsatellite markers for population analysis of a tephritid pest species, Bactrocera tryoniMol. Ecol.714891495CrossRefPubMedGoogle Scholar
  18. Lehmann, T., Hawley, W.A., Kamau, L., Fontenille, D., Simard, F., Collins, F.H. 1996Genetic differentiation of Anopheles gambiae populations from East to West Africa: comparison of microsatellite and allozyme lociHeredity69101111Google Scholar
  19. Loukas, M. 1989

    Population genetic studies of fruit flies of economic importance, especially medfly and olive fruit fly, using electrophoretic methods

    Loxdale, H.D.Hollander, J. eds. Electrophoretic Studies on Agricultural PestsClarendon PressOxford69102
    Google Scholar
  20. Loukas, M., Economopoulos, A.P., Zouros, E., Vergini, Y. 1985Genetic changes in artificially reared colonies of the olive fruit fly (Diptera: Tephritidae)Ann. Entomol. Soc. Am.78159165Google Scholar
  21. Mantel, N. 1967The detection of disease clustering and a generalized regression approachCancer Res.27209220PubMedGoogle Scholar
  22. Mazomenos,  B.E. 1989

    Dacus oleae

    Robinson, A.S. Hooper, G. eds. World Crop Pests. Vol.3B Elsevier Science Publishers B.V.Amsterdam169177
    Google Scholar
  23. Meixner, M.D., McPheron, B.A., Silva, J.G., Gasparich, G.E., Sheppard, W.S. 2002The Mediterranean fruit fly in California: evidence for multiple introductions and persistent populations based on microsatellite and mitochondrial DNA variabilityMol. Ecol.11891899CrossRefPubMedGoogle Scholar
  24. Nardi, F., Carapelli, A., Dallai, R., Frati, F. 2003The mitochondrial genome of the olive fly Bactrocera oleae: two haplotypes from distant geographical locationsInsect Mol. Biol.12605611CrossRefPubMedGoogle Scholar
  25. Nei, M. 1972Genetic distance between populationsAm. Nat.106283292CrossRefGoogle Scholar
  26. Ochando, M.D., Reyes, A. 2000Genetic population structure in olive fly Bactrocera oleae (Gmelin): gene flow and patterns of geographic differentiationJ. Appl. Entomol.124177183CrossRefGoogle Scholar
  27. Rubinsztein, D.C., Amos, W., Leggo, J., Goodburn, S., Jain, S., Li, S.H., Margolis, R.L., Ross, C.A., Ferguson-Smith, M.A. 1995Microsatellite evolution – evidence for directionality and variation in rate between speciesNat. Genet.10337343CrossRefPubMedGoogle Scholar
  28. Tsakas, S.C., Krimbas, C.B. 1975How many genes are selected in populations of Dacus oleaeGenetics79675679PubMedGoogle Scholar
  29. Tsakas, S.C., Zouros, E. 1980Genetic differences among natural and laboratory-reared populations of the olive fruit fly Dacus oleae (Diptera: Tephritidae)Entomol. Exp. Appl.28268276CrossRefGoogle Scholar
  30. Waples, R.S. 1987A multispecies approach to the analysis of gene flow in marine shore fishesEvolution41385400Google Scholar
  31. Wetterstrand, K.A., 1997. Microsatellite polymorphism and divergence in worldwide populations of Drosophila melanogaster and D. simulans. Master’s Thesis, Cornell University, Ithaca, NYGoogle Scholar
  32. Yeh F.C., T. Boyle, Y. Rongcai, Z. Ye & J.M. Xiyan, 1999. POPGENE VERSION 1.31 Microsoft Window-based Freeware for Population Genetic Analysis. http://www. ualberta.ca/∼ fyeh/Google Scholar
  33. Yu, H., Frommer, M., Robson, M.K., Meats, A.W., Shearman, D.C.A., Sved, J.A. 2001Microsatellite analysis of the Queensland fruit fly Bactrocera tryoni (Diptera: Tephritidae) indicates spatial structuring: implications for population controlBull. Entomol. Res.91139147PubMedGoogle Scholar
  34. Zheng, L., 1997. Microsatellite mapping of insect genomes, pp. 321–328, in Molecular Biology of Insect Disease Vectors: A Methods Manual, edited by J.M. Crampton, C.B. Beard & C. Louis, Chapman & HallGoogle Scholar
  35. Zouros, E., Krimbas, C.B. 1969The genetics of Dacus oleae. III. Amount of variation at two esterase loci in a Greek populationGenet. Res.14249258PubMedGoogle Scholar
  36. Zouros Loukas, E. M. (1989)

    Biochemical and colonization genetics of Dacus oleae (Gmelin)

    Robinson, A.S.Hooper,  G. eds. Fruit Flies: Their Biology, Natural Enemies and ControlElsevier Science PublishersAmsterdam7587
    Google Scholar

Copyright information

© Springer 2005

Authors and Affiliations

  • A.A. Augustinos
    • 1
  • Z. Mamuris
    • 2
  • E.E. Stratikopoulos
    • 1
  • S. D’Amelio
    • 3
  • A. Zacharopoulou
    • 1
  • K.D. Mathiopoulos
    • 1
  1. 1.Department of BiologyUniversity of Patras26504Greece
  2. 2.Department of Biochemistry and BiotechnologyUniversity of Thessaly41221Greece
  3. 3.Department of Public Health SciencesUniversity of Rome “La Sapienza”RomaItaly

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