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Genetica

, Volume 128, Issue 1–3, pp 167–175 | Cite as

Accumulation of chloroplast DNA sequences on the Y chromosome of Silene latifolia

  • Eduard Kejnovsky
  • Zdenek Kubat
  • Roman Hobza
  • Martina Lengerova
  • Shusei Sato
  • Satoshi Tabata
  • Kiichi Fukui
  • Sachihiro Matsunaga
  • Boris Vyskot
Article

Abstract

Silene latifolia is a model dioecious plant with heteromorphic sex chromosomes. The Y chromosome is the largest in this species. Theoretical models propose an accumulation of repetitive DNA sequences in non-recombining parts of the Y chromosome. In this study, we isolated a BAC7H5 clone preferentially hybridizing to the Y chromosome of S. latifolia. Sequence analysis revealed that this BAC7H5 contains part of the chloroplast genome, indicating that these chloroplast sequences have accumulated on the Y chromosome and also may contribute to its large size. We constructed Y chromosome- and X chromosome-specific libraries and screened them to find Y- and/or X-linked copies of chloroplast sequences. Sequence analysis revealed higher divergence of a non-genic region of the chloroplast sequences located on the Y chromosome while genic regions tested showed only very low (max 0.9%) divergence from their chloroplast homologues.

Keywords

accumulation chloroplast DNA Silene latifolia Y chromosome 

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Copyright information

© Springer 2006

Authors and Affiliations

  • Eduard Kejnovsky
    • 1
  • Zdenek Kubat
    • 1
  • Roman Hobza
    • 1
  • Martina Lengerova
    • 1
  • Shusei Sato
    • 2
  • Satoshi Tabata
    • 2
  • Kiichi Fukui
    • 3
  • Sachihiro Matsunaga
    • 3
  • Boris Vyskot
    • 1
  1. 1.Laboratory of Plant Developmental GeneticsInstitute of Biophysics, Academy of Sciences of the Czech RepublicBrnoCzech Republic
  2. 2.Kazusa DNA Research InstituteChibaJapan
  3. 3.Department of Biotechnology, Graduate School of EngineeringOsaka UniversityOsakaJapan

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