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Genetica

, Volume 124, Issue 2–3, pp 117–125 | Cite as

Molecular investigations of pathogenesis-related Bet v 1 homologues in Passiflora (Passifloraceae)

  • Carla Finkler
  • Carolina Giacomet
  • Valèria C. Muschner
  • Francisco M. SalzanoEmail author
  • Loreta B. Freitas
Article

Abstract

The major birch pollen allergen, Bet v 1, responsible for allergic reactions in many areas of the world, is homologous to a large number of pathogenesis-related proteins (PRs), identified as PR10. As part of a long-range investigation of these types of proteins and of evolution in Passiflora,DNA sequences from eight Bet v 1 homologue isoforms were obtained from five species of this genus in Brazil, and their sequences compared among themselves and with 30 others from 8 different species, classified in different taxonomic units. The objective was a first characterization of these PRs in wild passionflowers, and their use for evolutionary and applied investigations. High interspecific, but low intraspecific variability was observed, as expected from multigenic families subjected to concerted evolution. The relationships obtained both within Passiflora and between it and seven other genera probably best reflect functional similarities than evolutionary history.

Keywords

Bet v 1 homologues evolutionary relationships Passiflora pathogenesis-related proteins 

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References

  1. Breiteneder, H., Pettenburger, K., Bito, A., Valenta, R., Kraft, D., Rumpold, H., Scheiner, O., Breitenbach, M. 1989The gene coding for the major birch pollen allergen Bet v 1 is highly homologous to a pea disease resistance response geneEMBO J.819351938PubMedGoogle Scholar
  2. Breiteneder, H., Ferreira, F., Hoffmann-Sommergruber, K., Ebner, C., Breitenbach, M., Rumpold, H., Kraft, D., Scheiner, O. 1993Four recombinant isoforms of Cor a I, the major allergen of hazel pollen, show different IgE-binding propertiesEur. J. Biochem.212355362CrossRefPubMedGoogle Scholar
  3. Bronner, R., Westphal, E., Dereger, F. 1991PR proteins in Solanum dulcamara resistant to the gall mite Aceria cladophthirusPhysiol. Mol. Plant Pathol.3893104Google Scholar
  4. Bufe, A., Spangfort, M.D., Kahlert, H., Schlaak, M., Becker, W.M. 1996The major birch pollen allergen, Bet v 1, shows ribonuclease activityPlanta199413415CrossRefPubMedGoogle Scholar
  5. Cervi, A.C. 1997Passifloraceae do BrasilEstudo do gênero Passiflora L., subgênero Passiflora. Fontqueria45192Google Scholar
  6. Cooper, D.N. 1999Human Gene EvolutionBios Scientific PublishersOxfordGoogle Scholar
  7. Cote, F., Cutt, J.R., Asselin, A., Klessig, D.F. 1991PR acidic-1,3-glucanase genes of tobacco are regulated by both stress and developmental signalMol. Plant-Microbe Interact.4173181PubMedGoogle Scholar
  8. Daniels, C.H., Fristensky, B., Wagoner, W., Hadwinger, L.A. 1987Pea genes associated with non-host disease resistance to Fusarium are also active in race-specific disease resistance to PseudomonasPlant Mol. Biol.8309316CrossRefGoogle Scholar
  9. Edreva, A.M. 1990Induction of PR proteins in tobacco leaves by physiological (non-pathogenic) disordersJ. Exp. Bot.41701703Google Scholar
  10. Faber, C., Lindemann, A., Sticht, H., Ejchart, A., Kungl, A., Susani, M., Frank, R.W., Kraft, D., Breitenbach, M., Rösch, P. 1996Secondary structure and tertiary fold of the birch pollen allergen Betv1 in solutionJ. Biol. Chem.2711924319250PubMedGoogle Scholar
  11. Freitas, L.B., Koehler, P., Salzano, F.M. 2003aPathogenesis-related proteins in Brazilian wheat genotypes: protein induction and partial gene sequencingCienc. Rural33497500Google Scholar
  12. Freitas, L.B., Bonatto, S.L., Salzano, F.M. 2003bEvolutionary implications of the intra and interspecific molecular variability of pathogenesis-related proteinsBraz. J. Biol.63437448CrossRefGoogle Scholar
  13. Friedl-Hajek, R., Radauer, C., O’Riordain, G., Hoffmann-Sommergruber, K., Leberl, K., Scheiner, O., Breiteneder, H. 1999New Betv1 isoforms including a naturally occurring truncated form of the protein derived from Austrian birch pollenMol. Immunol.36639645CrossRefPubMedGoogle Scholar
  14. Fristensky, B., Horovitz, D., Hadwiger, L.A. 1988cDNA sequences for pea disease resistance response genesPlant Mol. Biol.11713715CrossRefGoogle Scholar
  15. Giavina-Bianchi, P.F.,Jr., Castro, F.F.M., Machado, M.L.S., Duarte, A.J.S. 1997Occupational respiratory allergic disease induced by Passiflora alata and Rhamnus purshianaAnn. Allergy Immunol.79449454Google Scholar
  16. Hammond-Kosack, K.E., Legrand, M., Geoffroy, P., Fritig, B. 1987Biological function of PR proteins. Four PR proteins of tobacco have - 1,3-glucanase activityEMBO J.632093212Google Scholar
  17. Hanfrey, C., Five, M., Buchanan-Wollaston, V. 1996Leaf senescence in Brassica napusExpression of genes coding pathogenesis-related proteins. Plant Mol. Biol.30597609Google Scholar
  18. Hoffmann-Sommergruber, K., Vanek-Krebitz, M., Radauer, C., Wen, J., Ferreira, F., Scheiner, O., Breiteneder, H. 1997Genomic characterization of members of the Betv1 family: genes coding for allergens and pathogenesis-related proteins share intron positionsGene19791100PubMedGoogle Scholar
  19. Iturriaga, E.A., Leech, M.J., Barratt, D.H.P., Wang, T.L. 1994Two ABA-responsive proteins from pea (Pisum sativumL.) are closely related to intracellular pathogenesis-related proteinsPlant Mol. Biol.24235240CrossRefPubMedGoogle Scholar
  20. Judd, W.S., Campbell, C.S., Kellogg, E.A., Stevens, P.F. 1999Plant Systematics. A Phylogenetic ApproachSinauerSunderlandGoogle Scholar
  21. Kumar, S., Tamura, K., Nei, M. 1993MEGA (Molecular Genetic Analysis), version 1.1Pennsylvania State UniversityUniversity ParkGoogle Scholar
  22. Lotan, T., Fluhr, R. 1990Function and regulated accumulation of plant PR proteinsSymbiosis83346Google Scholar
  23. Lucas, J., Camacho-Henriques, A., Lottspeich, F., Henschen, A., Sanger, H.L. 1985Amino acid sequence of the PR leaf protein p14 from viroid-infected tomato reveals a new type of structurally unfamiliar proteinsEMBO J.427452749Google Scholar
  24. Malamy, J.J., Carr, P., Klessig, D.F., Raskin, I. 1990Salicylic acid is a likely endogenous signal in the resistance response of tobacco to viral infectionScience25010021004Google Scholar
  25. Matton, D.P., Brisson, N. 1989Cloning, expression and sequence conservation of pathogenesis-related gene transcripts of potatoMol. Plant-Microbe Interact.2325331PubMedGoogle Scholar
  26. Mayer, R.T., Shapiro, J.P., Berdis, E., Hearn, C.J., McCollum, T.G., McDonald, R.E., Doostdar, H. 1995Citrus rootstock responses to herbivory by larvae of the sugarcane rootstock borer weevel (Diaprepes abbreviatus)Physiol. Plant.94164173CrossRefGoogle Scholar
  27. Moiseyev, G.P., Beintema, J.J., Fedoreyeva, L.I., Yakovlev, G.I. 1994High sequence similarity between a ribonuclease from ginseng calluses and fungus-elicited proteins from parsley indicates that intracellular pathogenesis-related proteins are ribonucleasesPlanta193470472CrossRefPubMedGoogle Scholar
  28. Moiseyev, G.P., Fedoreyeva, L.I., Zhuravlev, Y.N., Yasnetskaya, E., Jekel, P.A., Beintema, J.P. 1997Primary structures of two ribonucleases from ginseng calluses. New members of the PR-10 family of intracellular pathogenesis-related plant proteinsFEBS Lett.407207210CrossRefPubMedGoogle Scholar
  29. Muschner, V.C., Lorenz, A.P., Cervi, A.C., Bonatto, S.L., Souza-Chies, T.T., Salzano, F.M., Freitas, L.B. 2003A first molecular phylogenetic analysis in Passiflora (Passifloraceae)Am. J. Bot.9012291238Google Scholar
  30. Neudecker, P., Schweimer, K., Nerkamp, J., Scheurer, S., Vieths, S., Sticht, H., Rösch, P. 2001Allergic cross-reactivity made visible. Solution structure of the major cherry allergen Pru av 1J. Biol. Chem.2762275622763CrossRefPubMedGoogle Scholar
  31. Nei, M., Kumar, S. 2000Molecular Evolution and PhylogeneticsOxford University PressOxfordGoogle Scholar
  32. Rahimi, S., Perry, R.N., Wright, D.W. 1996Identification of pathogenesis-related proteins in leaves of potato plants infected with potato cyst nematodes, Globodera speciesPhysiol. Mol. Plant Pathol.494959CrossRefGoogle Scholar
  33. Roby, D., Broglie, K., Cressman, R. 1990Activation of a bean chitinase promotor in transgenic tobacco plants by phytopathogenic fungiPlant Cell29991007CrossRefPubMedGoogle Scholar
  34. Roy, A., Frascaria, N., MacKay, J., Bousquet, J. 1992Segregating random amplified polymorphic DNAs (RAPDs) in Betula alleghaniensisTheor. Appl. Genet.85173180CrossRefGoogle Scholar
  35. Scherer, N.M, C.E. Thompson, L.B. Freitas, S.L. Bonatto & F.M. Salzano, 2005. Patterns of molecular evolution in pathogenesis-related proteins (in press).Google Scholar
  36. Somssich, I.E., Schmelzer, E., Bollmann, J., Hahlbrock, K. 1986Rapid activation by fungal elicitor of genes encoding pathogenesis-related proteins in cultured parsley cellsProc. Nat. Acad. Sci. USA8324272430Google Scholar
  37. Somssich, I.E., Schmelzer, E., Kawalleck, P., Hahlbrock, K. 1988Gene structure and in situ transcript localization of pathogenesis-related protein 1 in parsleyMol. Gen. Genet.2139398CrossRefPubMedGoogle Scholar
  38. Swoboda, I., Dang, T.C.H., Heberle-Bors, E., Vicente, O. 1995Expression of Bet v 1, the major birch pollen allergen, during anther developmentAn in situ hybridization study. Protoplasma187103110Google Scholar
  39. Tamura, K 1998MEBoot 2.0 ProgramDepartment of Biology, Tokyo Metropolitan UniversityTokyoGoogle Scholar
  40. Thompson, J.D., Higgins, D.G., Gibson, T.J. 1994Clustal W: improving the sensitivity of progressive multiple sequence alignment through sequence weighting, position-specific gap penalties and weight matrix choiceNucl. Acid. Res.2246734680Google Scholar
  41. Vande Rhee, M.D., Kan, J.A.L., Jaen, M.T.G., Bol, J.F. 1990Analysis of regulatory elements involved in the induction of two tobacco genes by salicylate treatment and virus infectionPlant Cell2357366Google Scholar
  42. Walter, M.H., Liu, J.W., Grand, C., Lamb, C., Hess, D. 1990Bean pathogenesis-related (PR) proteins deduced from elicitor-induced transcripts are members of a ubiquitous new class of conserved PR proteins including pollen allergensMol. Gen. Genet.222353360CrossRefPubMedGoogle Scholar
  43. Wen, J., Vanek-Krebitz, M., Hoffmann-Sommergruber, K., Scheiner, O., Breiteneder, H. 1997The potential of Betv1 homologues, a nuclear multigene family, as phylogenetic markers in flowering plantsMol. Phylogenet. Evol.8317333PubMedGoogle Scholar
  44. Westphal, E., Dereger, F., Bronner, R. 1990The gall mite Aceria cladophthirus.I. Life cycle, survival outside the gall and symptoms expression on susceptible or resistant Solanum dulcamaraplantsExp. Appl. Acarol.9183200Google Scholar

Copyright information

© Springer 2005

Authors and Affiliations

  • Carla Finkler
    • 1
  • Carolina Giacomet
    • 1
  • Valèria C. Muschner
    • 1
  • Francisco M. Salzano
    • 1
    Email author
  • Loreta B. Freitas
    • 1
  1. 1.Departamento de Genètica, Instituto de BiociênciasUniversidade Federal do Rio Grande do SulPorto AlegreBrazil

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