, Volume 124, Issue 2–3, pp 117–125 | Cite as

Molecular investigations of pathogenesis-related Bet v 1 homologues in Passiflora (Passifloraceae)

  • Carla Finkler
  • Carolina Giacomet
  • Valèria C. Muschner
  • Francisco M. SalzanoEmail author
  • Loreta B. Freitas


The major birch pollen allergen, Bet v 1, responsible for allergic reactions in many areas of the world, is homologous to a large number of pathogenesis-related proteins (PRs), identified as PR10. As part of a long-range investigation of these types of proteins and of evolution in Passiflora,DNA sequences from eight Bet v 1 homologue isoforms were obtained from five species of this genus in Brazil, and their sequences compared among themselves and with 30 others from 8 different species, classified in different taxonomic units. The objective was a first characterization of these PRs in wild passionflowers, and their use for evolutionary and applied investigations. High interspecific, but low intraspecific variability was observed, as expected from multigenic families subjected to concerted evolution. The relationships obtained both within Passiflora and between it and seven other genera probably best reflect functional similarities than evolutionary history.


Bet v 1 homologues evolutionary relationships Passiflora pathogenesis-related proteins 


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Copyright information

© Springer 2005

Authors and Affiliations

  • Carla Finkler
    • 1
  • Carolina Giacomet
    • 1
  • Valèria C. Muschner
    • 1
  • Francisco M. Salzano
    • 1
    Email author
  • Loreta B. Freitas
    • 1
  1. 1.Departamento de Genètica, Instituto de BiociênciasUniversidade Federal do Rio Grande do SulPorto AlegreBrazil

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