Advertisement

Genetica

, Volume 122, Issue 1, pp 51–64 | Cite as

Implementing Large-Scale ENU Mutagenesis Screens in North America

  • Amander T. Clark
  • Daniel Goldowitz
  • Joseph S. Takahashi
  • Martha Hotz Vitaterna
  • Sandra M. Siepka
  • Luanne L. Peters
  • Wayne N. Frankel
  • George A. Carlson
  • Janet Rossant
  • Joseph H. Nadeau
  • Monica J. Justice
Article

Abstract

A step towards annotating the mouse genome is to use forward genetics in phenotype-driven screens to saturate the genome with mutations. The purpose of this article is to highlight the new projects in North America that are focused on isolating mouse mutations after ENU mutagenesis and phenotype screening.

balancer chromosomes Human Genome Project Mouse Genome Project mouse mutagenesis phenotype screens 

Preview

Unable to display preview. Download preview PDF.

Unable to display preview. Download preview PDF.

References

  1. Andretic, R., S. Chaney & J. Hirsh, 1999. Requirement of circadian genes for cocaine sensitization in Drosophila. Science 285(5430): 1066–1068.PubMedGoogle Scholar
  2. Antoch, M. P., E. J. Song, A. M. Chang, M. H. Vitaterna, Y. Zhao, L. D. Wilsbacher, A. M. Sangoram, D. P. King, L. H. Pinto & J. S. Takahashi, 1997. Functional identi ca-tion of the mouse circadian Clock gene by transgenic BAC rescue. Cell 89(4): 655–667.PubMedGoogle Scholar
  3. Aszterbaum, M., J. Epstein, A. Oro, V. Douglas, P. E. LeBoit, M. P. Scott & E. H. Epstein Jr, 1999a. Ultraviolet and ionizing radiation enhance the growth of BCCs and trichoblastomas in patched heterozygous knockout mice. Nat. Med. 5(11): 1285–1291.PubMedGoogle Scholar
  4. Aszterbaum, M., J. Beech & E. H. Epstein Jr., 1999b. Ultravi-olet radiation mutagenesis of hedgehog pathway genes in basal cell carcinomas. J. Invest. Derm. Symp. Proc. 4(1): 41–45. Review.Google Scholar
  5. Evans, M. J., M. B. Carlton & A. P. Russ, 1997. Gene trapping and functional genomics. Trends Genet. 13: 370–374.PubMedGoogle Scholar
  6. Gross, C., L. Santarelli, D. Brunner, X. Zhuang & R. Hen, 2000. Altered fear circuits in 5–HT(1A) receptor KO mice. Biol. Psychiat. 48: 1157–1163.PubMedGoogle Scholar
  7. Fahrer, A. M., J. F. Bazan, P. Papathanasiou, K. A. Nelms & C. C. Goodnow, 2001. A genomic view of immunology. Nature 409(6822): 836–838.PubMedGoogle Scholar
  8. Gu, H., J. D. Marth, P. C. Orban, H. Mossmann & K. Rajewsky, 1994. Deletion of DNA polymerase b gene segment in T cells using cell type-specific gene targeting. Science 265: 103–106.PubMedGoogle Scholar
  9. He, C., R. K. Zalups, D. A. Henderson, G. E. Striker & L. J. Striker, 1995. Molecular analysis of spontaneous glomerulosclerosis in Os/+mice, a model with reduced nephron mass. Am. J. Physiol. 269(2 Pt 2): F266–273.PubMedGoogle Scholar
  10. Hrabe de Angelis, M. H., H. Flaswinkel, H. Fuchs, B. Rathkolb, D. Soewarto, S. Marschall, S. Heffner, W. Pargent, K. Wuensch, M. Jung, A. Reis, T. Richter, F. Alessandrini, T. Jakob, E. Fuchs, H. Kolb, E. Kremmer, K. Schaeble, B. Rollinski, A. Roscher, C. Peters, T. Meitinger, T. Strom, T. Steckler, F. Holsboer, T. Klopstock, F. Gekeler, C. Schindewolf, T. Jung, K. Avraham, H. Behrendt, J. Ring, A. Zimmer, K. Schughart, K. Pfeffer, E. Wolf & R. Balling, 2000. Genome-wide, large-scale production of mutant mice by ENU mutagenesis. Nat. Genet. 25(4): 444–447.PubMedGoogle Scholar
  11. Justice, M. J., 2000. Capitalizing on large-scale mouse muta-genesis screens. Nat. Rev. Genet. 1: 111–115.Google Scholar
  12. Justice, M. J., J. K. Noveroske, J. S. Weber, B. Zheng & A. Bradley, 1999. Mouse ENU mutagenesis. Hum. Mol. Genet. 8(10): 1955–1963.PubMedGoogle Scholar
  13. Kile, B. T., K. E. Hentges, A. T. Clark, H. Nakamura, A. P. Salinger, B. Liu, N. Box, D. W. Stockton, R. L. Johnson, R. R. Behringer, A. Bradley & M. J. Justice, 2003. Func-tional genetic analysis of mouse Chromosome 11. Nature 485: 81–86.Google Scholar
  14. King, D. P., Y. Zhao, A. M. Sangoram, L. D. Wilsbacher, M. Tanaka, M. P. Antoch, T. D. Steeves, M. H. Vitaterna, J. M. Kornhauser, P. L. Lowrey, F. W. Turek & J. S. Takahashi, 1997. Positional cloning of the mouse circadian Clock gene. Cell 89(4): 641–653.PubMedGoogle Scholar
  15. Lakso, M., B. Sauer, B. Mosinger Jr., E. J. Lee, R. W. Manning, S. H. Yu, K. L. Mulder & H. Westphal, 1992. Targeted oncogene activation by site speci c recombination in transgenic mice. Proc. Natl. Acad. Sci. USA 89: 6232–6236.PubMedGoogle Scholar
  16. Lowrey, P. L. & J. S. Takahashi, 2000. Genetics of the mam-malian circadian system: photic entrainment, circadian pacemaker mechanisms, and post translational regulation. Annu. Rev. Genet. 34: 533–562.PubMedGoogle Scholar
  17. Nadeau, J. H., R. Balling, G. Barsh, D. Beier, S. D. Brown, M. Bucan, S. Camper, G. Carlson, N. Copeland, J. Eppig, C. Fletcher, W. N. Frankel, D. Ganten, D. Goldowitz, C. Goodnow, J. L. Guenet, G. Hicks, M. H. de Angelis, I. Jackson, H. J. Jacob, N. Jenkins, D. Johnson, M. J. Justice, S. Kay, D. Kingsley, H. Lehrach, T. Magnuson, M. Meisler, A. Poustka, E. M. Rinchik, J. Rossant, L. B. Russell, J. Schimenti, T. Shiroishi, W. C. Skarnes, P. Soriano, W. Stanford, J. S. Takahashi, W. Wurst & A. Zimmer, 2001, Functional annotation of mouse genome sequences. Science 291: 5507: 1251–1255.PubMedGoogle Scholar
  18. Nolan, P. M., J. Peters, M. Strivens, D. Rogers, J. Hagan, N. Spurr, I. C. Gray, L. Vizor, D. Brooker, E. Whitehill, R. Washbourne, T. Hough, S. Greenaway, M. Hewitt, X. Liu, S. McCormack, K. Pickford, R. Selley, C. Wells, Z. Tymowska-Lalanne, P. Roby, P. Glenister, C. Thornton, C. Thaung, J. A. Stevenson, R. Arkell, P. Mburu, P. R. Hardisty, A. Kiernan, A. Erven, K. P. Steel, S. Voegeling, J. L. Guenet, C. Nickols, R. Sadri, M. Nasse, A. Isaacs, K. Davies, M. Browne, E. M. Fisher, J. Martin, S. Rastan, S. D. M. Brown & J. Hunter, 2000a. A systematic, genome-wide, phenotype-driven mutagenesis program for gene function studies in the mouse. Nat. Genet. 25(4): 440–443.PubMedGoogle Scholar
  19. Nolan, P. M., J. Peters, L. Vizor, M. Strivens, R. Washbourne, T. Hough, C. Wells, P. Glenister, C. Thornton, J. Martin, E. Fisher, D. Rogers, J. C. Hagan, I. Gray, J. Wood, N. Spurr, M. Browne, S. Rastan, J. Hunter & S. D. Brown, 2000b. Implementation of a large-scale ENU mutagenesis program: towards increasing the mouse mutant resource. Mamm. Genome 11(7): 500–506.PubMedGoogle Scholar
  20. O’ Brien, T. P., 2002. Mutagenesis and genetic screens in the mouse, pp. 93–108 in Systematic Evaluation of the Mouse Eye, edited by R. S. Smith, CRC Press, New York.Google Scholar
  21. O’ Bryan, T., H. Weiher, H. G. Rennke, S. Kren & T. H. Hostetter, 2000. Course of renal injury in the Mpv17–de cient transgenic mouse. J. Am. Soc. Nephrol. 11(6): 1067–1074.PubMedGoogle Scholar
  22. Orban, P. C., D. Chui & J. D. Marth, 1992. Tissue and site-speci c DNA recombination in transgenic mice. Proc. Natl. Acad. Sci. USA 89: 6861–6236.PubMedGoogle Scholar
  23. Pargent, W., S. Heffner, K. F. Schable, D. Soewarto, H. Fuchs & M. Hrabe de Angelis, 2000. MouseNet database: digital management of a large-scale mutagenesis project. Mamm. Genome 11: 590–593.PubMedGoogle Scholar
  24. Peters, L. L., E. M. Cheever, H. R. Ellis, P. Magnani, K. L. Svenson, R. V. Smith & M. Bogue, 2002. Large scale, high throughput screening for coagulation and hematologic phenotypes in mice. Physiol. Genomics 11: 185–193.PubMedGoogle Scholar
  25. Rathkolb, B., T. Decker, E. Fuchs, D. Soewarto, C. Fella, S. Heffner, W. Pargent, R. Wanke, R. Balling, M. Hrabe de Angelis, H. J. Kolb & E. Wolf, 2000. The clinical-chemical screen in the Munich ENU Mouse Mutagenesis Project: screening for clinically relevant phenotypes. Mamm. Genome 11: 543–546.PubMedGoogle Scholar
  26. Rogers, D. C., E. M. Fisher, S. D. M. Brown, J. Peters, A. J. Hunter & J. E. Martin, 1997. Behavioral and functional analysis of mouse phenotype SHIRPA, a proposed protocol for comprehensive phenotype assessment. Mamm. Genome 8(10): 711–713.PubMedGoogle Scholar
  27. Roths, J. B., W. B. Foxworth, M. J. McArthur, C. A. Montgo-mery & A. B. Kier, 1999. Spontaneous and engineered mutant mice as models for experimental and comparative pathology: history, comparison and developmental tech-nology. Lab. Anim. Sci. 49: 12–34.PubMedGoogle Scholar
  28. Soewarto, D., C. Fella, A. Teubner, B. Rathkolb, W. Pargent, S. Heffner, S. Marschall, E. Wolf, R. Balling & M. Hrabe de Angelis, 2000. The large-scale Munich ENU-mouse-mutagenesis screen. Mamm. Genome 11: 507–510.PubMedGoogle Scholar
  29. Taylor, B. A., 1999. Mouse Mapping, in Mouse Genetics and Transgenics: A Practical Approach, edited by Ian Jackson and Cathy Abbott, Oxford University Press, Oxford.Google Scholar
  30. Takahashi, J. S., L. H. Pinto & M. H. Vitaterna, 1994. Forward and reverse genetic approaches to behavior in the mouse. Science 264(5166): 1724–1733.PubMedGoogle Scholar
  31. Vitaterna, M. H., D. P. King, A. M. Chang, J. M. Kornhauser, P. L. Lowrey, J. D. McDonald, W. F. Dove, L. H. Pinto, F. W. Turek & J. S. Takahashi, 1994. Mutagenesis and mapping of a mouse gene, Clock, essential for circadian behavior. Science 264(5159): 719–725.PubMedGoogle Scholar
  32. Xiaohong, M., Y. Fujiwara & S. H. Orkin, 1999. Improved reporter strain for monitoring Cre recombinase-mediated DNA excisions in mice. Proc Natl. Acad. Sci. USA 96: 5037–5042.PubMedGoogle Scholar
  33. Zheng, B., M. Sage, W. W. Cai, D. M. Thompson, B. C. Tavsanli, Y. C. Cheah & A. Bradley, 1999. Engineering a mouse balancer chromosome. Nat. Genet. 22(4): 375–378.PubMedGoogle Scholar
  34. Ziyadeh, F. N., B. B. Hoffman, D. C. Han, M. C. Iglesias-De La Cruz, S. W. Hong, M. Isono, S. Chen, T. A. McGowan & K. Sharma, 2000. Long-term prevention of renal insu. ciency, excess matrix gene expression, and glomerularmesangial matrix expansion by treatment with monoclonal anti-transforming growth factor-beta antibody in db/db diabetic mice. P. Natl. Acad. Sci. USA 97: 8015–8020.Google Scholar

Copyright information

© Kluwer Academic Publishers 2004

Authors and Affiliations

  • Amander T. Clark
    • 1
  • Daniel Goldowitz
    • 2
  • Joseph S. Takahashi
    • 3
  • Martha Hotz Vitaterna
    • 3
  • Sandra M. Siepka
    • 3
  • Luanne L. Peters
    • 4
  • Wayne N. Frankel
    • 5
  • George A. Carlson
    • 6
  • Janet Rossant
    • 7
  • Joseph H. Nadeau
    • 8
  • Monica J. Justice
    • 9
  1. 1.Department of Molecular and Human GeneticsBaylor College of MedicineUSA
  2. 2.Tennessee Mouse Genome CenterUSA
  3. 3.Center for Functional GenomicsNorthwestern UniversityUSA
  4. 4.Center for Mouse Models of HeartLung, Blood and Sleep Disorders, The Jackson LaboratoryUSA
  5. 5.Neuroscience Mutagenesis FacilityThe Jackson LaboratoryUSA
  6. 6.McLaughlin Research Institute for Biomedical SciencesUSA
  7. 7.The Center for Modeling Human DiseaseCanada
  8. 8.Case Western Reserve University/University Hospitals of ClevelandUSA
  9. 9.Department of Molecular and Human GeneticsBaylor College of MedicineUSA

Personalised recommendations