Effects of dietary myo-inositol on growth, antioxidative capacity, and nonspecific immunity in skin mucus of taimen Hucho taimen fry

  • Chang’an Wang
  • Shaoxia Lu
  • Jinnan Li
  • Liansheng Wang
  • Haibo Jiang
  • Yang Liu
  • Hongbai LiuEmail author
  • Shicheng Han
  • Jiasheng Yin


In this study, the effects of dietary myo-inositol on the skin mucosal immunity and growth of taimen (Hucho taimen) fry were determined. Triplicate groups of 500 fish (initial weight 5.58 ± 0.15 g) were fed different diets containing graded levels of myo-inositol (28.75, 127.83, 343.83, 565.81, and 738.15 mg kg−1) until satiation for 56 days. Thereafter, the nonspecific skin mucus immune parameters, antioxidative capacity, and growth performance were measured. The skin mucus protein and the activities of alkaline phosphatase were significantly higher than those in the control group (P < 0.05). However, there were no significant differences in lysozyme activity among the treatments (P > 0.05). The antimicrobial activity and minimum inhibitory concentration of the skin mucus were increased significantly by myo-inositol supplementation (P < 0.05). The superoxide dismutase, catalase, and glutathione peroxidase activities were significantly elevated in the treatment groups (P < 0.05), whereas the malondialdehyde contents were significantly decreased (P < 0.05). Low-level myo-inositol (28.75 mg kg−1) led to a significantly lower weight gain, feed efficiency, condition factor, and survival rate compared with the other treatments (P < 0.05). In conclusion, dietary myo-inositol deficiency (28.75 mg kg−1) adversely affects the skin mucus immune parameters, antioxidative capacity, and growth performance of Hucho taimen fry.


Hucho taimen Myo-inositol Immunity Skin mucus Antioxidative capacity Growth 


Funding information

This study was supported by the Natural Science Funds of Heilongjiang (YQ2019C036), the China Agriculture Research System (CARS-46), the Science and Technology Project of Guizhou Province (20162502, 20162511), the Guizhou Science and Technology Plan Project (QKHZC20172532), the Guizhou Technology Innovation Team Project (QKHRCTD20154016), and the Beijing Sturgeon & Trout Innovation Team (BAIC08-2018).

Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflict of interest.

Ethical approval

All procedures involving animals were in accordance with the ethical standards of the institutional and/or national research committee.

Statement of informed consent

Informed consent was obtained from all individual participants included in the study.


  1. NRC (National Research Council) (2011) Nutrient requirements of fish and shrimp. National Academies Press, Washington DC, pp 206–207Google Scholar
  2. Adel M, Amiri AA, Zorriehzahra J, Nematolahi A, Esteban MÁ (2015) Effects of dietary peppermint (Mentha piperita) on growth performance, chemical body composition and hematological and immune parameters of fry Caspian white fish (Rutilus frisii kutum). Fish Shellfish Immunol 45(2):841–847CrossRefGoogle Scholar
  3. Ángeles Esteban M (2012) An overview of the immunological defenses in fish skin, ISRN ImmunologyGoogle Scholar
  4. Aukema HM, Holub BJ (1994) Inositol and pyrroloquinoline quinone. In: Shils ME, Olson JA, Shike M (Eds.), Modern Nutrition in Health and Disease, (8th ed.). Lea and Febiger, Philadelphia, pp 466-472.Google Scholar
  5. Dash S, Das SK, Samal J, Thatoi HN (2018) Epidermal mucus, a major determinant in fish health: a review. Iran J Vet Res 19(2):72PubMedPubMedCentralGoogle Scholar
  6. Diao SQ, Huang Z, Chen SS, Niu J, Li ZJ, Ding X, Lin HZ (2010) Effect of dietary inositol on growth, feed utilization and blood biochemical parameters for juvenile barramundi (Lates calcarifer Bloch). Am J Agric Biol Sci 5(3):370–375CrossRefGoogle Scholar
  7. Ellis AE (1999) Immunity to bacteria in fish. Fish Shellfish Immunol 9(4):291–308CrossRefGoogle Scholar
  8. Faggio C, Fazio F, Marafioti S, Arfuso F, Piccione G (2015) Oral administration of gum arabic: effects on haematological parameters and oxidative stress markers in Mugil cephalus. Iran J Fish Sci 14(1):60–72Google Scholar
  9. Fast MD, Sims DE, Burka JF, Mustafa A, Ross NW (2002) Skin morphology and humoral non-specific defence parameters of mucus and plasma in rainbow trout, coho and Atlantic salmon. Comp Biochem Physiol A Mol Integr Physiol 132(3):645–657CrossRefGoogle Scholar
  10. Ghehdarijani MS, Hajimoradloo A, Ghorbani R, Roohi Z (2016) The effects of garlic-supplemented diets on skin mucosal immune responses, stress resistance and growth performance of the Caspian roach (Rutilus rutilus) fry. Fish Shellfish Immunol 49:79–83CrossRefGoogle Scholar
  11. Harris JE, Hunt S (1975) The fine structure of the epidermis of two species of salmonid fish, the Atlantic salmon (Salmo solar L.) and the brown trout (Salmo trutta L.). Cell Tissue Res 157(4):553–565CrossRefGoogle Scholar
  12. Hauser G, Finelli VN (1963) The biosynthesis of free and phosphatide myo-inositol from glucose by mammalian tissue slices. J Biol Chem 238:3224–3228PubMedGoogle Scholar
  13. Hellio C, Pons AM, Beaupoil C, Bourgougnon N, Le Gal Y (2002) Antibacterial, antifungal and cytotoxic activities of extracts from fish epidermis and epidermal mucus. Int J Antimicrob Agents 20(3):214–219CrossRefGoogle Scholar
  14. Hernandez LHH, Barrera TC, Mejia JC, Mejia GC, Del Carmen M, Dosta M, De Lara Andrade R, Sotres JAM (2010) Effects of the commercial probiotic Lactobacillus casei on the growth, protein content of skin mucus and stress resistance of juveniles of the porthole livebearer Poecilopsis gracilis (Poecilidae). Aquac Nutr 16(4):407–411CrossRefGoogle Scholar
  15. Hoseinifar SH, Sharifian M, Vesaghi MJ, Khalili M, Esteban MÁ (2014) The effects of dietary xylooligosaccharide on mucosal parameters, intestinal microbiota and morphology and growth performance of Caspian white fish (Rutilus frisii kutum) fry. Fish Shellfish Immunol 39(2):231–236CrossRefGoogle Scholar
  16. Hoseinifar SH, Zou HK, Van Doan H, Kolangi Miandare H, Hoseini SM (2018) Evaluation of some intestinal cytokines genes expression and serum innate immune parameters in common carp (Cyprinus carpio) fed dietary loquat (Eriobotrya japonica) leaf extract. Aquac Res 49(1):120–127CrossRefGoogle Scholar
  17. Jiang WD, Feng L, Liu Y, Jiang J, Zhou XQ (2009) Growth, digestive capacity and intestinal microflora of juvenile Jian carp (Cyprinus carpio var Jian) fed graded levels of dietary inositol. Aquac Res 40(8):955–962CrossRefGoogle Scholar
  18. Jiang WD, Feng L, Liu Y, Jiang J, Hu K, Li SH, Zhou XQ (2010) Lipid peroxidation, protein oxidant and antioxidant status of muscle, intestine and hepatopancreas for juvenile Jian carp (Cyprinus carpio var Jian) fed graded levels of myo-inositol. Food Chem 120(3):692–697CrossRefGoogle Scholar
  19. Kim HW, Kim JH, An HS, Park KK, Kim BK, Park T (2003) Myo-inositol restores the inflammation-induced down-regulation of taurine transport by the murine macrophage cell line, RAW 2647. Life Sci 73(19):2477–2489CrossRefGoogle Scholar
  20. Kitamura S (1967) Studies on vitamin requirements of rainbow trout II the deficiency symptoms of fourteen kinds of vitamin. Bull Jap Soc Sci Fish 33:1120–1125CrossRefGoogle Scholar
  21. Lauriano ER, Pergolizzi S, Capillo G, Kuciel M, Alesci A, Faggio C (2016) Immunohistochemical characterization of toll-like receptor 2 in gut epithelial cells and macrophages of goldfish Carassius auratus fed with a high-cholesterol diet. Fish Shellfish Immunol 59:250–255CrossRefGoogle Scholar
  22. Lowry OH, Rosebrough NJ, Farr AL, Randall RJ (1951) Protein measurement with the Folin phenol reagent. J Biol Chem 193:265–275PubMedPubMedCentralGoogle Scholar
  23. Palaksha KJ, Shin GW, Kim YR, Jung TS (2008) Evaluation of non-specific immune components from the skin mucus of olive flounder (Paralichthys olivaceus). Fish Shellfish Immunol 24(4):479–488CrossRefGoogle Scholar
  24. Peres H, Lim C, Klesius PH (2004) Growth, chemical composition and resistance to Streptococcus iniae challenge of juvenile Nile tilapia (Oreochromis niloticus) fed graded levels of dietary inositol. Aquaculture 235(1–4):423–432CrossRefGoogle Scholar
  25. Rao V, Marimuthu K, Kupusamy T, Rathinam X, Arasu MV, Al-Dhabi NA, Arockiaraj J (2015) Defense properties in the epidermal mucus of different freshwater fish species, aquaculture, aquarium. Conservation and Legislation 8(2):184–194Google Scholar
  26. Roosta Z, Hoseinifar SH (2016) The effects of crowding stress on some epidermal mucus immune parameters, growth performance and survival rate of tiger barb (Pentius tetrazona). Aquac Res 47(5):1682–1686CrossRefGoogle Scholar
  27. Ross NW, Firth KJ, Wang A, Burka JF, Johnson SC (2000) Changes in hydrolytic enzyme activities of naive Atlantic salmon Salmo salar skin mucus due to infection with the salmon louse Lepeophtheirus salmonis and cortisol implantation. Dis Aquat Org 41(1):43–51CrossRefGoogle Scholar
  28. Saurabh S, Sahoo PK (2008) Lysozyme: an important defence molecule of fish innate immune system. Aquac Res 39(3):223–239CrossRefGoogle Scholar
  29. Sheikhzadeh N, Heidarieh M, Pashaki AK, Nofouzi K, Farshbafi MA, Akbari M (2012a) Hilyses®, fermented Saccharomyces cerevisiae, enhances the growth performance and skin non-specific immune parameters in rainbow trout (Oncorhynchus mykiss). Fish Shellfish Immunol 32(6):1083–1087CrossRefGoogle Scholar
  30. Sheikhzadeh N, Pashaki AK, Nofouzi K, Heidarieh M, Tayefi-Nasrabadi H (2012b) Effects of dietary Ergosan on cutaneous mucosal immune response in rainbow trout (Oncorhynchus mykiss). Fish Shellfish Immunol 32(3):407–410CrossRefGoogle Scholar
  31. Subramanian S, MacKinnon SL, Ross NW (2007) A comparative study on innate immune parameters in the epidermal mucus of various fish species. Comp Biochem Physiol B Biochem Mol Biol 148(3):256–263CrossRefGoogle Scholar
  32. Tabrez S, Ahmad M (2009) Effect of wastewater intake on antioxidant and marker enzymes of tissue damage in rat tissues: implications for the use of biochemical markers. Food Chem Toxicol 47(10):2465–2478CrossRefGoogle Scholar
  33. Taee HM, Hajimoradloo A, Hoseinifar SH, Ahmadvand H (2017) Dietary myrtle (Myrtus communis L.) improved non-specific immune parameters and bactericidal activity of skin mucus in rainbow trout (Oncorhynchus mykiss) fingerlings. Fish Shellfish Immunol 64:320–324CrossRefGoogle Scholar
  34. Taoka Y, Maeda H, Jo JY, Jeon MJ, Bai SC, Lee WJ, Yuge K, Koshio S (2006) Growth, stress tolerance and non-specific immune response of Japanese flounder Paralichthys olivaceus to probiotics in a closed recirculating system. Fisheries Sci 72(2):310–321CrossRefGoogle Scholar
  35. Vennila R, Kumar KR, Kanchana S, Arumugam M, Vijayalakshmi S, Balasubramaniam T (2011) Preliminary investigation on antimicrobial and proteolytic property of the epidermal mucus secretion of marine stingrays. Asian Pac J Trop Biomed 1(2):S239–S243CrossRefGoogle Scholar
  36. Wang CA, Liu H, Li J, Wang L, Zhao Z, Luo L, Xu Q (2018) Effects of dietary myo-inositol on growth, chemical composition and plasma chemistry of Amur sturgeon Acipenser schrenckii. Aquac Int 26(6):1481–1492CrossRefGoogle Scholar
  37. Zhang T, Yue XJ (2014) Research progress of secretion mechanisms, components and function of fishes mucus. J Anhui Agric Sci 42(22):7445–7448Google Scholar
  38. Zhang CN, Zhang JL, Ren HT, Zhou BH, Wu QJ, Sun P (2017) Effect of tributyltin on antioxidant ability and immune responses of zebrafish (Danio rerio). Ecotox Environ Safe 138:1–8CrossRefGoogle Scholar

Copyright information

© Springer Nature B.V. 2020

Authors and Affiliations

  1. 1.Heilongjiang River Fisheries Research InstituteChinese Academy of Fishery SciencesHarbinPeople’s Republic of China
  2. 2.College of Animal ScienceGuizhou UniversityGuiyangPeople’s Republic of China

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