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The effect of Spirulina platensis meal on antioxidant gene expression, total antioxidant capacity, and lipid peroxidation of rainbow trout (Oncorhynchus mykiss)

  • Mahdi TeimouriEmail author
  • Sakineh Yeganeh
  • Ghodrat Rahimi Mianji
  • Mojtaba Najafi
  • Soleiman Mahjoub
Article

Abstract

The study was conducted to investigate the effect of diets containing 0, 2.5, 5, 7.5, and 10% S. platensis meal on total antioxidant capacity (TAC) and lipid peroxidation, as well as the expression of two antioxidant enzyme genes (SOD and CAT) in rainbow trout (Oncorhynchus mykiss). One hundred and eighty fish, with an average initial weight of 101 ± 8 g, were cultured for 10 weeks. At the end of experiment, lipid peroxidation significantly decreased in serum of fish fed with S. platensis and fish fed with 5, 7.5, and 10% microalgae showed a significantly lower value compared to control and 2.5%. Inclusion of 7.5 and 10% S. platensis in diet also decreased lipid peroxidation in liver. TAC significantly increased with increasing level of S. platensis. Expression level of superoxide dismutase (SOD) and catalase (CAT) genes significantly increased in the fish liver after administration of microalgae and fish fed with 10% S. platensis in diet showed the highest level compared to the other treatments. The present study reveals that inclusion of 10% S. platensis in diet can decrease oxidative stress in rainbow trout. Therefore, S. platensis can be used as potential antioxidant for fish farming.

Keywords

Spirulina platensis Total antioxidant capacity (TAC) Lipid peroxidation Antioxidant gene expression Rainbow trout (Oncorhynchus mykiss

Notes

Acknowledgements

The authors would like to thank research council of the Sari Agricultural Sciences and Natural Resources University (Project No. 08-1393-01), Sari, Iran, for supporting this research. The authors would like to acknowledge the Iranian National Elite Foundation. We are grateful to Dr. Evangeline Foronda for English editing.

Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflict of interest.

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Copyright information

© Springer Nature B.V. 2019

Authors and Affiliations

  • Mahdi Teimouri
    • 1
    Email author
  • Sakineh Yeganeh
    • 1
  • Ghodrat Rahimi Mianji
    • 2
  • Mojtaba Najafi
    • 2
  • Soleiman Mahjoub
    • 3
  1. 1.Department of Fisheries, Faculty of Animal Science and FisheriesSari Agricultural Sciences and Natural Resources UniversitySariIran
  2. 2.Department of Animal Science, Faculty of Animal Science and FisheriesSari Agricultural Science and Natural Resources UniversitySariIran
  3. 3.Clinical Biochemistry, Cellular and Molecular Biology Research Center, Health Research InstituteBabol University of Medical SciencesBabolIran

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