Fish Physiology and Biochemistry

, Volume 45, Issue 6, pp 1933–1940 | Cite as

Hemoglobin deoxygenation and methemoglobinemia prevent regulatory volume decrease in crucian carp (Carassius carassius) red blood cells

  • A. Y. AndreyevaEmail author
  • A. A. Soldatov
  • A. I. Krivchenko
  • I. V. Mindukshev
  • S. Gambaryan


Fish red blood cells (RBCs) exhibit an oxygen-dependent regulatory volume decrease (RVD) in hypoosmotic environment. In higher vertebrates, membrane-associated hemoglobin is involved in the regulation of osmotic ion movements across the cellular membrane. However, whether the hemoglobin conformational state plays a role in the regulation of osmotic responses in fish red blood cells is still not fully understood. We found that changes in hemoglobin conformation influence the pattern of the regulatory volume decrease in Carassius carassius red blood cells. In oxygenated cells (96.4 ± 3.7% oxygenated hemoglobin), the volume recovery was completed within 125 min. Deoxygenation of hemoglobin (96.5 ± 2.7% of deoxygenated hemoglobin) inhibited the volume decrease in hyposmotically swollen red blood cells. Reoxygenation restored regulatory volume decrease in cells within 5 min. Induced methemoglobinemia (48.4 ± 1.8% of methemoglobin and 41.3 ± 2.3% of deoxygenated hemoglobin) blocked the process of volume recovery and significantly decreased osmotic stability of red blood cells.


Hemoglobin conformational state Osmolarity Volume regulation Red blood cells Teleosts 



These studies were supported by the Ministry of Science and Higher Education of the Russian Federation (No. АААА-А18-118012290371-3; No. AAAA-A18-118021490093-4).

Compliance with ethical standards

All procedures using fish were accomplished in accordance with the European Communities Council Directive (2010/63/EU) and approved by the local Institutional Animal Care and Use Committee (protocol #28 from 15.02. 2018).


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Copyright information

© Springer Nature B.V. 2019

Authors and Affiliations

  1. 1.The A.O. Kovalevsky Institute of Marine Biological ResearchRussian Academy of SciencesMoscowRussia
  2. 2.Sechenov Institute of Evolutionary Physiology and BiochemistryRussian Academy of SciencesSt. PetersburgRussia
  3. 3.Department of Cytology and HistologySt. Petersburg State UniversitySt. PetersburgRussia

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