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Fish Physiology and Biochemistry

, Volume 45, Issue 6, pp 1845–1865 | Cite as

Genomic structure and characterization of growth hormone receptors from golden pompano Trachinotus ovatus and their expression regulation by feed types

  • Yinyin Liang
  • Huayang Guo
  • Bo Liu
  • Kecheng Zhu
  • Shigui Jiang
  • Dianchang ZhangEmail author
Article

Abstract

In this study, sequence analysis showed that ToGHR1 and ToGHR2 encoded polypeptides of 577 and 588 amino acids, respectively. Bioinformatics analysis showed that both ToGHR1 and ToGHR2 contain FN3 domains and transmembrane domains, which have glycosylation and phosphorylation sites. The exons of ToGHR1 and exons 4–10 of ToGHR2 are homologous to exons 2 and 4–9 in Homo sapiens genes, respectively. Only 3 SSR sites in ToGHR1 have SSR polymorphisms, and ToGHR2 has no SSR polymorphisms. ToGHR1 and ToGHR2 have high homology with GHR1 and GHR2 of many fish by BLAST. qRT-PCR was used to examine the expression profile of ToGHR mRNA in 12 normal liver and intestine tissue samples from 3 feed-type groups. The results showed that ToGHR is expressed in all 12 tissues, especially liver and muscle tissues, which showed higher ToGHR expression than that in other tissues (p < 0.05). Experiments on feed-type groups may indicate that high levels of LC-PUFA in squid bait can promote ToGHR1 expression and simultaneously inhibit ToGHR2 expression in the liver tissue. In addition, the high levels of LC-PUFA in food could inhibit intestinal ToGHR1 expression, and the intermediate levels may promote intestinal ToGHR1 expression. However, the unsaturated fatty acid content in the food does not affect the expression of intestinal ToGHR2.

Keywords

Feed GHR Promoter SSR Quantitative real-time PCR Trachinotus ovatus 

Notes

Acknowledgments

This study was supported by the Chinese Agriculture Research System (CARS-47), Guangdong Provincial Special Fund for Modern Agriculture Industry Technology Innovation Teams, Hainan province departmental basic budget project (2017), the National Infrastructure of Fishery Germplasm Resources Project (2018DKA30407) and the Science and Technology Infrastructure Construction Project of Guangdong Province (2019B030316030).

Supplementary material

10695_2019_682_MOESM1_ESM.docx (1.9 mb)
ESM 1 (DOCX 1991 kb)

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Copyright information

© Springer Nature B.V. 2019

Authors and Affiliations

  • Yinyin Liang
    • 1
    • 2
  • Huayang Guo
    • 1
    • 3
  • Bo Liu
    • 1
    • 2
  • Kecheng Zhu
    • 1
    • 3
  • Shigui Jiang
    • 1
    • 3
  • Dianchang Zhang
    • 1
    • 3
    Email author
  1. 1.Key Laboratory of South China Sea Fishery Resources Exploitation and Utilization, Ministry of Agriculture and Rural AffairsSouth China Sea Fisheries Research Institute, Chinese Academy of Fishery SciencesGuangzhouChina
  2. 2.College of Fisheries and Life ScienceShanghai Ocean UniversityShanghaiChina
  3. 3.Guangdong Provincial Engineer Technology Research Center of Marine Biological Seed IndustryGuangzhouChina

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