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Fish Physiology and Biochemistry

, Volume 45, Issue 6, pp 1791–1800 | Cite as

In vitro antioxidant enzyme activity and sperm motility at different temperatures in sterlet Acipenser ruthenus and rainbow trout Oncorhynchus mykiss

  • Hadiseh DadrasEmail author
  • Viktoriya Dzyuba
  • Amin Golpour
  • Miaomiao Xin
  • Borys Dzyuba
Article

Abstract

Influence of in vitro temperature on sperm antioxidant enzyme activity, thiobarbituric acid-reactive substance (TBARS) content and motility parameters was evaluated in sterlet Acipenser ruthenus and rainbow trout Oncorhynchus mykiss. Sperm activation was conducted at 4, 14 and 24 °C in both species. Duration of motility was significantly longer at 4 °C than at 14 and 24 °C in both species. At 60 s post-activation, the velocity of sterlet spermatozoa was highest at 24 °C. This trend continued to 420 s post-activation. In rainbow trout, at 10 s post-activation, the highest velocity was observed at 14 °C. Significantly higher catalase activity was seen at 4 °C in both species. No significant difference in spermatozoon superoxide dismutase activity among temperatures was observed. In sterlet, TBARS content was significantly higher at 24 °C compared to other temperatures, but, in rainbow trout, it was highest at 4 °C. The results presume species-specific level of antioxidant enzyme activity and TBARS content at studied temperatures.

Keywords

Antioxidant enzyme Fish Sperm function Temperature 

Notes

Acknowledgments

The study was financially supported by the Ministry of Education, Youth and Sports of the Czech Republic, projects: CENAKVA (LM2018099), Biodiversity (CZ.02.1.01./0.0/0.0/16_025/0007370) and GAJU (122/2019/Z) and by the Ministry of Agriculture of the Czech Republic, project NAZV QK1710310.

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Copyright information

© Springer Nature B.V. 2019

Authors and Affiliations

  1. 1.Faculty of Fisheries and Protection of Waters, South Bohemia in Research Center of Aquaculture and Biodiversity of Hydrocenoses, Research Institute of Fish Culture and HydrobiologyUniversity of South Bohemia in Ceske BudejoviceVodňanyCzech Republic
  2. 2.State Key Laboratory of Freshwater Ecology and Biotechnology, Institute of Hydrobiology Chinese Academy of Sciences, Innovation Academy for Seed DesignWuhanChina

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