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Molecular cloning and expression pattern of IGFBP-2a in black porgy (Acanthopagrus schlegelii) and evolutionary analysis of IGFBP-2s in the species of Perciformes

  • Xinyi Zhang
  • Zhiyong Zhang
  • Zhenpeng Yu
  • Jiayi Li
  • Shuyin ChenEmail author
  • Ruijian Sun
  • Chaofeng Jia
  • Fei Zhu
  • Qian Meng
  • Shixia XuEmail author
Article

Abstract

Insulin-like growth factor-binding protein-2 (IGFBP-2) plays a key role in regulating growth and development by its affinity with insulin-like growth factors (IGFs). In this study, we cloned the coding sequence (CDS) of IGFBP-2a from the black porgy (Acanthopagrus schlegelii) muscle and identified that the full-length CDS of IGFBP-2a was 882 bp. Real-time quantitative PCR revealed that IGFBP-2a was most abundant in the liver of the black porgy and backcross breed (F1♀×black porgy♂) but remained lower in each tested tissue in self-cross breed (F1♀×F1♂). In addition, the IGFBP-2a expression in the liver of three breeds showed a negative correlation with their growth rates, indicating that the IGFBP-2a played a growth-inhibiting role in the three breeds. We further identified 810 bp IGFBP-2b gene from the draft genome of black porgy. Finally, we examined the IGFBP-2a and IGFBP-2b genes by scanning the genomes of the species of Perciformes and found the IGFBP-2 gene duplication took place earlier than the divergence of perciform species. Interestingly, six positively selected sites were detected in both Perciformes IGFBP-2 genes, although both genes were identified to be under purifying selection. Specially, these positively selected sites were located in the functional domains, suggesting these sites played key roles in the growth of Perciformes. Our study partially explains the molecular basis for the prepotency in black porgy hybrids, which will provide guidance for their cultivation in the future.

Keywords

IGFBP-2 Acanthopagrus schlegelii Cloning Tissue distribution Gene evolution 

Notes

Acknowledgments

This work was financially supported by the Key Project for Jiangsu Agricultural New Variety Innovation (PZCZ201744), Key Research and Development (Modern Agriculture) Program of Jiangsu Province (BE2016326), Independent Innovation of Agricultural Science and Technology of Jiangsu Province (CX(17)2021), Aquatic Sanxin Engineering Project of Jiangsu Province (Y2016-23), the National Natural Science Foundation of China (NSFC) (grant no. 31570379), and the Priority Academic Program Development of Jiangsu Higher Education Institutions (PAPD). We thank Dr. Ran Tian for his technical supports.

Supplementary material

10695_2019_665_MOESM1_ESM.docx (19 kb)
ESM 1 (DOCX 19 kb)
10695_2019_665_Fig6_ESM.png (66 kb)
Online Resource 2

Phylogenetic tree of Perciformes IGFBP-2s reconstructed by Neighbor-Joining method (NJ). Numbers on branches represent the bootstrap values (%). The black porgy IGFBP-2s are noted by ▲. The accession numbers of downloaded IGFBP-2s are described in Table 3. (PNG 66 kb)

10695_2019_665_MOESM2_ESM.tiff (70.3 mb)
High Resolution Image (TIFF 72017 kb)

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Copyright information

© Springer Nature B.V. 2019

Authors and Affiliations

  1. 1.Marine Fisheries Research Institute of Jiangsu ProvinceNantongChina
  2. 2.Jiangsu Key Laboratory for Biodiversity and Biotechnology, College of Life SciencesNanjing Normal UniversityNanjingChina

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