Immuno-physiological and antioxidant responses of Siberian sturgeon (Acipenser baerii) fed with different levels of olive pomace

  • Akbar Banavreh
  • Mehdi SoltaniEmail author
  • Abolghasem Kamali
  • Mohammad A. Yazdani-Sadati
  • Mehdi Shamsaie


Nowadays, use of the natural plant derivatives as an immunostimulant in aquaculture is becoming more useful than antibiotics which are destructive to target animals, consumers, and the environment. The present study aimed to evaluate the efficacy of dietary substitution of wheat flour at 2, 5, 7.5, and 10% of olive pomace (OP2, OP5, OP7.5, and OP10) in some immune-physiological and antioxidant variables of yearling Siberian sturgeon (Acipenser baerii) weighing 165 ± 6.8 g at 19.1 ± 1.52 °C for 56 days. Fifteen fish per tank were randomly accommodated in triplicates per treatment and the control group was also included. No significant difference was seen in hematological indices including red blood cells (RBS) and immunocompetent cell population sizes among the treated groups (P > 0.05). Also, malondialdehyde (MDA) activity and superoxide dismutase (SOD) in serum remained unaffected in different treatments (P > 0.05). Total protein (TP), high-density lipoprotein (HDL), catalase (CT), and lysozyme were affected by dietary OP levels (P < 0.05), and the highest value was observed at the OP5 diet, but no influence was seen in serum IgM by OP. These data show that application of olive pomace as a partial substitute of wheat flour in the diet of Siberian sturgeon is feasible with stimulatory and antioxidant functions.


Acipenser baerii Antioxidant status Blood biochemical Hematological value Immune response Olive pomace 



The authors thank the research team at the International Sturgeon Research Institute and Science and Research at Islamic Azad University. This work was supported by the University of Tehran, Islamic Azad University, and University of Murdoch.


  1. Adel M, Yeganeh S, Dadar M, Sakai M, Dawood MAO (2016) Effects of dietary Spirulina platensis on growth performance, humoral and mucosal immune responses and disease resistance in juvenile great sturgeon (Huso huso Linnaeus, 1754). Fish Shellfish Immunol 56:436–444. CrossRefGoogle Scholar
  2. Aebi H (1984) Catalase. Methods Enzymol 105(1947):121–126. CrossRefGoogle Scholar
  3. Almaida-Pagán PF, Hernández MD, García García B, Madrid JA, De Costa J, Mendiola P (2007) Effects of total replacement of fish oil by vegetable oils on n-3 and n-6 polyunsaturated fatty acid desaturation and elongation in sharpsnout seabream (Diplodus puntazzo) hepatocytes and enterocytes. Aquaculture 272(1–4):589–598. CrossRefGoogle Scholar
  4. Amro B, Aburjai T, Al-Khalil S (2002) Antioxidative and radical scavenging effects of olive cake extract. Fitoterapia 73(6):456–461. CrossRefGoogle Scholar
  5. AOAC (1990) Official methods of analysis, 15th edn. Association of Official Analytical Chemists, Washington, DCGoogle Scholar
  6. Araujo M, Pimentel FB, Alves RC, Oliveira MB (2015) Phenolic compounds from olive mill wastes: health effects, analytical approach and application as food antioxidants. Trends Food Sci Technol 45(2):200–211. CrossRefGoogle Scholar
  7. Arciuli M, Fiocco D, Fontana S, Arena MP, Frassanito MA, Gallone A (2017) Administration of a polyphenol-enriched feed to farmed sea bass (Dicentrarchus labrax L.): kidney melanomacrophages response. Fish Shellfish Immunol 68:404–410. CrossRefGoogle Scholar
  8. Banavreh A, Soltani M, Kamali A, Yazdani Sadati MA, Shamsaie M (2018) Effects of olive pomace on growth performance, digestibility, body composition and fatty acid profile in yearling Siberian sturgeon , Acipenser baerii ( Brandt 1896). Aquac Nutr 25(2):333–342. CrossRefGoogle Scholar
  9. Beauchamp C, Fridovich I (1971) Superoxide dismutase. Anal Biochem 44:276–287. CrossRefGoogle Scholar
  10. Blaxhall PC, Daisley KW (1973) Routine hematological methods for use with fish blood. J Fish Biol 5:771–781. CrossRefGoogle Scholar
  11. Bradford MM (1976) A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein dye-binding. Anal Biochem 72:248–254. CrossRefGoogle Scholar
  12. Buege J, Aust S (1978) Microsomal lipid peroxidation. Meth Enzymol 52:302–310. CrossRefGoogle Scholar
  13. Chen S, Guo Y, Espe M, Yang F, Fang W, Wan M, Tian L (2018) Growth performance, haematological parameters, antioxidant status and salinity stress tolerance of juvenile Pacific white shrimp (Litopenaeus vannamei) fed different levels of dietary myo-inositol. Aquac Nutr 24(5):1527–1539. CrossRefGoogle Scholar
  14. Dadras H, Hayatbakhsh MR, Shelton WL, Golpour A (2016) Effects of dietary administration of Rose hip and Safflower on growth performance, haematological, biochemical parameters and innate immune response of Beluga, Huso huso (Linnaeus, 1758). Fish Shellfish Immunol 59:109–114. CrossRefGoogle Scholar
  15. Dal Bosco A, Mourvaki E, Cardinali R, Servili M, Sebastiani B, Ruggeri S, Castellini C (2012) Effect of dietary supplementation with olive pomaces on the performance and meat quality of growing rabbits. Meat Sci 92(4):783–788. CrossRefGoogle Scholar
  16. Dimitrios B (2006) Sources of natural phenolic antioxidants. Trends Food Sci Technol 17:505–512. CrossRefGoogle Scholar
  17. Ellis AE (1990) Lysozyme assays. In: Stolen JS (ed) Techniques in fish immunology. SOS publication, Fair Haven, pp 101–103Google Scholar
  18. Eslamloo K, Falahatkar B (2014) Variations of some physiological and immunological parameters in Siberian sturgeon (Acipenser baerii, Brandt, 1869) subjected to an acute stressor. J Appl Anim Welf Sci 17(1):29–42. CrossRefGoogle Scholar
  19. Eslamloo K, Falahatkar B, Yokoyama S (2012) Effects of dietary bovine lactoferrin on growth, physiological performance, iron metabolism and non-specific immune responses of Siberian sturgeon Acipenser baerii. Fish Shellfish Immunol 32(6):976–985. CrossRefGoogle Scholar
  20. Gatlin DM, Barrows FT, Brown P, Dabrowski K, Gaylord TG, Hardy RW, Wurtele E (2007) Expanding the utilization of sustainable plant products in aquafeeds: a review. Aquac Res 38(6):551–579. CrossRefGoogle Scholar
  21. Ghanbari R, Anwar F, Alkharfy KM, Gilani AH, Saari N (2012) Valuable nutrients and functional bioactives in different parts of olive (Olea europaea L.)-a review. Int J Mol Sci 13(3):3291–3340CrossRefGoogle Scholar
  22. Harmantepe FB, Aydin F, Doğan G (2016) The potential of dry olive cake in a practical diet for juvenile hybrid tilapia, Oreochromis niloticus × Oreochromis aereus. Aquac Nutr 22(5):956–965. CrossRefGoogle Scholar
  23. Hasanpour S, Salati AP, Falahatkar B, Azarm HM (2017) Effects of dietary green tea (Camellia sinensis L.) supplementation on growth performance, lipid metabolism, and antioxidant status in a sturgeon hybrid of Sterlet (Huso huso ♂ × Acipenser ruthenus ♀) fed oxidized fish oil. Fish Physiol Biochem 43(5):1315–1323. CrossRefGoogle Scholar
  24. Hosseini SV, Kenari AA, Regenstein JM, Rezaei M, Nazari RM, Moghaddasi M, Grant AAM (2010) Effects of alternative dietary lipid sources on growth performance and fatty acid composition of Beluga sturgeon, Huso huso, juveniles. J World Aquacult Soc 41(4):471–489. CrossRefGoogle Scholar
  25. Hwang JH, Lee SW, Rha SJ, Yoon HS, Park ES, Han KH, Kim SJ (2013) Dietary green tea extract improves growth performance, body composition, and stress recovery in the juvenile black rockfish, Sebastes schlegeli. Aquac Int 21(3):525–538. CrossRefGoogle Scholar
  26. Karantonis HC, Tsantila N, Stamatakis G, Samiotaki M, Panayotou G, Antonopoulou S, Demopoulos CA (2007) Bioactive polar lipids in olive oil, pomace and waste byproducts. Food Biochem 32:443–459. CrossRefGoogle Scholar
  27. Li M, Chen L, Li E, Yu N, Ding Z, Chen Y, Qin JG (2015) Growth, immune response and resistance to Aeromonas hydrophila of darkbarbel catfish, Pelteobagrus vachelli (Richardson), fed diets with different linolenic acid levels. Aquac Res 46(4):789–800. CrossRefGoogle Scholar
  28. Li Q, Zhu HY, Wei JJ, Zhang F, Li EC, Du ZY, Chen LQ (2016) Effects of dietary lipid sources on growth performance, lipid metabolism and antioxidant status of juvenile Russian sturgeon Acipenser gueldenstaedtii. Aquac Nutr 23(3):1–11. Google Scholar
  29. Long M, Lin W, Hou J, Guo H, Li L, Li D, Yang F (2017) Dietary supplementation with selenium yeast and tea polyphenols improve growth performance and nitrite tolerance of Wuchang bream (Megalobrama amblycephala). Fish Shellfish Immunol 68(1):74–83. CrossRefGoogle Scholar
  30. Luo L, Ai L, Liang X, Hu H, Xue M, Wu X (2017) n-3 Long-chain polyunsaturated fatty acids improve the sperm, egg, and offspring quality of Siberian sturgeon (Acipenser baerii). Aquaculture 473:266–271. CrossRefGoogle Scholar
  31. Magrone T, Fontana S, Laforgia F, Dragone T, Jirillo E, Passantino L (2016) Administration of a polyphenol-enriched feed to farmed sea bass (Dicentrarchus labrax L.) modulates intestinal and spleen immune responses. Oxidative Med Cell Longev 68:1–11. CrossRefGoogle Scholar
  32. Makkar HPS, Francis G, Becker K (2007) Bioactivity of phytochemicals in some lesser-known plants and their effects and potential applications in livestock and aquaculture production systems. Animal 1(9):1371–1391. CrossRefGoogle Scholar
  33. Mohseni M, Ozório ROA (2014) Effects of dietary L-carnitine level on growth performance, body composition and antioxidant status in beluga (Huso huso L. 1758). Aquac Nutr 20(5):477–485. CrossRefGoogle Scholar
  34. Nasopoulou C, Stamatakis G, Demopoulos CA, Zabetakis I (2011) Effects of olive pomace and olive pomace oil on growth performance, fatty acid composition and cardio protective properties of gilthead sea bream (Sparus aurata) and sea bass (Dicentrarchus labrax). Food Chem 129(3):1108–1113. CrossRefGoogle Scholar
  35. Nasopoulou C, Zabetakis (2013) Agricultural and aquacultural potential of olive pomace. J Agric Sci 5(7):116–127Google Scholar
  36. Nasopoulou C, Gogaki V, Stamatakis G, Papaharisis L, Demopoulos CA, Zabetakis I (2013) Evaluation of the in vitro anti-atherogenic properties of lipid fractions of olive pomace, olive pomace enriched fish feed and gilthead sea bream (Sparus aurata) fed with olive pomace enriched fish feed. Mar Drugs 11(10):3676–3688. CrossRefGoogle Scholar
  37. Nunes MA, Pimente FB, Costa ASG, Alves RC, Oliveira MBPP (2016) Olive by-products for functional and food applications: challenging opportunities to face environmental constraints. Innov Food Sci Emerg Technol 35:139–148. CrossRefGoogle Scholar
  38. Porter LJ, Hrstich LN, Chan BG (1986) The conversion of proanthocyanidins and prodelphinidins to cyanidin and delpinidin. Phytochemistry 25:223–230. CrossRefGoogle Scholar
  39. Richter N, Siddhuraju P, Becker K (2003) Evaluation of nutritional quality of moringa (Moringa oleifera Lam.) leaves as an alternative protein source for Nile tilapia (Oreochromis niloticus L.). Aquaculture 217(1–4):599–611. CrossRefGoogle Scholar
  40. Sabzi E, Mohammadiazarm H, Salati AP (2017) Effect of dietary L-carnitine and lipid levels on growth performance, blood biochemical parameters and antioxidant status in juvenile common carp (Cyprinus carpio). Aquaculture 480:89–93. CrossRefGoogle Scholar
  41. Sakai M (1999) Current research status of fish immunostimulants [Review]. Aquaculture 172(1–2):63–92. CrossRefGoogle Scholar
  42. Sargent JR, Bell JG, Bell MV, Henderson RJ, Tocher DR (1992) The metabolism of phospholipids and polyunsaturated fatty acids in fish. Aquaculture 43:103–124. Google Scholar
  43. Şener E, Yildiz M, Savaş E (2005) Effects of dietary lipids on growth and fatty acid composition in Russian sturgeon (Acipenser gueldenstaedtii) juveniles. Turk J Vet Anim Sci 29(5):1101–1107Google Scholar
  44. Shalaby A, Khattab YA, Abdel Rahman AM (2006) Effects of garlic (Allium sativum) and chloramphenicol on growth performance, physiological parameters and survival of Nile tilápia (Oreochromis niloticus). J Venom Anim Toxins Incl Trop Dis 12(2):172–201. CrossRefGoogle Scholar
  45. Sharangi AB (2009) Medicinal and therapeutic potentialities of tea (Camellia sinensis L.) - a review. Food Res Int 42(5–6):529–535. CrossRefGoogle Scholar
  46. Sies H (1991) Role of reactive oxygen species in biological processes. Klin Wochenschr 69(21–23):965–968. CrossRefGoogle Scholar
  47. Siwicki AK, Anderson DP (1993) Nonspecific defense mechanisms assay in fish: II. Potential killing activity of neutrophils and macrophages, lysozyme activity in serum and organs and total immunoglobulin level in serum. Fish Disease Diagnosis and Prevention Methods. Wydaw- nictwo Instytutu Rybactwa Strodladowego, Olsztyn, Poland, pp 105–112Google Scholar
  48. Soltani M, Masouleh AS, Ahmadi M, Pourkazemi M, Taherimirghaed A (2015) Antibacterial activity, antibiotic susceptibility and probiotic use of lactic acid bacteria (LAB) in Persian sturgeon (Acipenser persicus). Iran J Aquat Anim Health 2(1):54–65Google Scholar
  49. Stoskoph MK (1993) Fish medicine. W.B. Saun- ders Company, Philadelphia, pp 115–122Google Scholar
  50. Subbotkina TA, Subbotkin MF (2003) Lysozyme content in organs and blood serum in various species in the Volga River. J Evol Biochem Physiol 39(5):537–546. CrossRefGoogle Scholar
  51. Vallejos-Vidal E, Reyes-López F, Teles M, MacKenzie S (2016) The response of fish to immunostimulant diets. Fish Shellfish Immunol 56:34–69. CrossRefGoogle Scholar
  52. Verlhac V, Obach A, Gabaudan J, Schuep W, Hole R (1998) Immunomodulation by dietary vitamin C and glucan in rainbow trout (Oncorhynchus mykiss). Fish Shellfish Immunol 8(6):409–424. CrossRefGoogle Scholar
  53. Wan J, Ge X, Liu B, Xie J, Cui S, Zhou M, Chen R (2014) Effect of dietary vitamin C on non-specific immunity andmRNA expression of three heat shock proteins (HSPs) in juvenile Megalobrama amblycephala under pH stress. Aquaculture 434:325–333. CrossRefGoogle Scholar

Copyright information

© Springer Nature B.V. 2019

Authors and Affiliations

  • Akbar Banavreh
    • 1
  • Mehdi Soltani
    • 2
    • 3
    Email author
  • Abolghasem Kamali
    • 1
  • Mohammad A. Yazdani-Sadati
    • 4
  • Mehdi Shamsaie
    • 1
  1. 1.Department of Fisheries, Faculty of Agriculture and Natural Resources, Science and Research BranchIslamic Azad UniversityTehranIran
  2. 2.Department of Aquatic Animal Health, Faculty of Veterinary MedicineUniversity of TehranTehranIran
  3. 3.Freshwater Fish Group and Fish Health Unit, School of Veterinary and Life SciencesMurdoch UniversityPerthAustralia
  4. 4.International Sturgeon Research Institute-Agricultural Research Education Organization (AREEO)RashtIran

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