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Dietary supplementation of Streptococcus faecalis benefits the feed utilization, antioxidant capability, innate immunity, and disease resistance of blunt snout bream (Megalobrama amblycephala)

  • Xiao-Qun Zhong
  • Ming-Yang Liu
  • Chao Xu
  • Wen-Bin Liu
  • Kenneth-Prudence Abasubong
  • Xiang-Fei LiEmail author
Article

Abstract

This study aimed to investigate the effects of Streptococcus faecalis on the growth performance, intestinal histology, antioxidant capability, innate immunity, and disease resistance of blunt snout bream Megalobrama amblycephala. Fish were fed five experimental diets containing 0 (SF0, control), 1 × 105 (SF1), 1 × 106 (SF2), 1 × 107 (SF3), and 1 × 108 cfu/g (SF4) of Streptococcus faecalis, respectively, for 10 weeks. After the feeding trial, fish were challenged by Aeromonas hydrophila with the cumulative mortality recorded during a period of 96 h. The results showed that WG and FI of fish both showed no significant difference (P > 0.05) among all the treatments. However, the FCR was significantly (P < 0.05) affected by Streptococcus faecalis levels with the lowest value observed in the SF2 group, whereas the opposite was true for intestinal microvillus length (P < 0.05). Dietary supplementation of 1 × 106 cfu/g Streptococcus faecalis significantly (P < 0.05) increased the hepatic activities of SOD, CAT, and GPx; plasma activities of LZM, MPO, ACP, and AKP; and the levels of C3, C4, and IgM of fish, compared with the control group. Similar results were also observed in the tissue expressions of Leap-I, Leap-II, muc2, and muc5b (P < 0.05), whereas the opposite was true for liver MDA contents and plasma NO levels (P < 0.05). At 96 h after challenge, the cumulative mortality of the control was significantly (P < 0.05) higher than that of the SF2 group, but it showed no statistical difference (P > 0.05) with that of the other treatments. These results indicated that dietary supplementation of 1 × 106 cfu/g Streptococcus faecalis could not only improve the feed utilization of blunt snout bream but also enhance its antioxidant capability, innate immunity, and disease resistance.

Keywords

Streptococcus faecalis Growth Innate immunity Disease resistance Blunt snout bream 

Notes

Acknowledgements

The authors also wanted to thank the following people for their technical assistance and help during fish husbandry and sampling: Xiufei Cao, Huajuan Shi, Jie Liu, Huihui Chen, Jiadai Liu, and Chenyuan Xu.

Funding information

The present study was funded by the National Technology System for Conventional Freshwater Fish Industries of China (CARS-45-14).

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Copyright information

© Springer Nature B.V. 2018

Authors and Affiliations

  • Xiao-Qun Zhong
    • 1
  • Ming-Yang Liu
    • 2
  • Chao Xu
    • 1
  • Wen-Bin Liu
    • 1
  • Kenneth-Prudence Abasubong
    • 1
  • Xiang-Fei Li
    • 1
    Email author
  1. 1.Key Laboratory of Aquatic Nutrition and Feed Science of Jiangsu Province, College of Animal Science and TechnologyNanjing Agricultural UniversityNanjingChina
  2. 2.Wuxi Fisheries CollegeNanjing Agricultural UniversityWuxiChina

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