Physiological and metabolic responses of juvenile Lophiosilurus alexandri catfish to air exposure
The present study aimed to evaluate the physiological and metabolic stress responses of juvenile Lophiosilurus alexandri submitted to an air exposure test. The subjects consisted of 72 juveniles. Blood samples were taken at: 0 h—fish not exposed to air; 0.5 h—fish shortly after exposure to air for 30 min (prior to returning to the tank); 1.5 h (90 min), 24, 48, and 96 h after the initiation of exposure to air for 30 min. After 96 h, survivorship was 100%. Cortisol and glucose levels were higher at 0.5 h, returning to baseline at 48 and 24 h, respectively. Lactate dehydrogenase levels were highest at 1.5 h after exposure to air, returning to normal values in 24 h. Several changes were recorded in gasometric blood values and electrolytes. With regard to hematology and blood chemistry, exposure to air did not affect globular volume and AST throughout the 96 h of the experiment. The values for alkaline phosphatase were highest at 0, 1.5, and 24 h. Total protein was similar between 0 and 1.5 h and lowest at 96 h, while ALT was highest at 0.5 h. Leukocytes were highest at 0.5, 1.5, 48, and 96 h, while erythrocytes were highest at 96 h. After 96 h, juvenile L. alexandri were able to reestablish the main indicators of stress (cortisol, glucose and lactate dehydrogenase), while other indicators (hematological, biochemical, and gasometric) exhibited compensatory variation for normal physiological re-establishment.
KeywordsPhysiological stress Blood gasometric analysis Management
We are grateful to Danilo Gonçalves Bastos for his technical assistance.
This work was financially supported by Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq-Brasil), Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES-Brasil), Fundação de Amparo à Pesquisa do Estado de Minas Gerais (FAPEMIG-Brasil). LUZ, R.K. received a research grant from the Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq No. 305048/2015-5) and from the Fundação de Amparo à Pesquisa do Estado de Minas Gerais (FAPEMIG No. PPM-00250/15).
Compliance with ethical standards
All experimental protocols were approved by the Committee for Ethics in Animal Experimentation of the Universidade Federal de Minas Gerais (approval reference number: 280/2016). The study complied with the ethical principles under which Experimental Physiology operates, and the experiments complied with the journal’s animal ethics principles and regulation checklist (Grundy 2015).
- Apha (1998) Standard methods for the examination of water and wastewater, 20th edn. American Public Health Association, American Water Works Association, Water Environmental Federation, WashingtonGoogle Scholar
- Baldisserotto B, Martos-Sitcha JA, Menezes CC, Toni C, Prati RL, Garcia LO, Salbego J, Mancera JM, Martínez-Rodríguez G (2014) The effects of ammonia and water hardness on the hormonal, osmoregulatory and metabolic responses of the freshwater silver catfish Rhamdia quelen. Aquat Toxicol 152:341–352. https://doi.org/10.1016/j.aquatox.2014.04.023 CrossRefGoogle Scholar
- Barton BA (2000) Salmonid fishes differ in their cortisol and glucose responses to handling and transport stress. N Am J Aquac 62:12–18. https://doi.org/10.1577/1548-8454(2000)062<0012:SFDITC>2.0.CO;2 CrossRefGoogle Scholar
- Becker AG, Luz RK, Mattioli CC, Nakayama CL, Silva WS, Leme FOP, Mendes HCPM, Heinzmann BM, Baldisserotto B (2017) Can the essential oil of Aloysia triphylla have anesthetic effect and improve the physiological parameters of the carnivorous freshwater catfish Lophiosilurus alexandri after transport? Aquaculture 481:184–190. https://doi.org/10.1016/j.aquaculture.2017.09.007 CrossRefGoogle Scholar
- Bezerra RF, Soares MCF, Santos AJG, Carvalho EVMM, Coelho LCBB (2014) Seasonality influence on biochemical and hematological indicators of stress and growth of pirarucu (Arapaima gigas), an Amazonian air-breathing fish. Sci World J 2014:1–6. https://doi.org/10.1155/2014/541278 CrossRefGoogle Scholar
- Buttle LG, Uglow RF, Coax IN (1996) The effect of emersion and handling on the nitrogen excretion rates of Clarias gariepinus. J Fish Biol 49:693–701. https://doi.org/10.1111/j.1095-8649.1996.tb00065.x Google Scholar
- Cordeiro NIS, Costa DC, Silva WS, Takata R, Miranda-Filho KC, Luz RK (2016) High stocking density during larviculture and effect of size and diet on production of juvenile Lophiosilurus alexandri Steindachner, 1876 (Siluriformes: Pseudopimelodidae). J Appl Ichthyol 32:61–66. https://doi.org/10.1111/jai.12963 CrossRefGoogle Scholar
- Costa DC, Silva WS, Melillo-Filho R, Miranda-Filho KC, Santos JCE, Luz RK (2015) Capture, adaptation and artificial control of reproduction of Lophiosilurus alexandri: a carnivorous freshwater species. Anim Reprod Sci 159:148–154. https://doi.org/10.1016/j.anireprosci.2015.06.009 CrossRefGoogle Scholar
- Damsgaard C, Phuong LM, Huong DTT, Jensen FB, Wang T, Bayley M (2015b) High affinity and temperature sensitivity of blood oxygen binding in Pangasianodon hypophthalmus due to lack of chloride-hemoglobin allosteric interaction. Am J Physiol Regul Integr Comp Physiol 308:907–915. https://doi.org/10.1152/ajpregu.00470.2014 CrossRefGoogle Scholar
- Flodmark LEW, Urke HA, Halleraker JH, Arnekleiv JV, Vøllestad LA, Poléo ABS (2002) Cortisol and glucose responses in juvenile brown trout subjected to a fluctuating flow regime in an artificial stream. J Fish Biol 60:238–248. https://doi.org/10.1111/j.1095-8649.2002.tb02401.x CrossRefGoogle Scholar
- Jain NC (1986) Schalm's veterinary hematology, 4th edn. Lea & Febiger, Philadelphia, p 1221Google Scholar
- Kitagawa AT, Costa LS, Paulino RR, Luz RK, Rosa PV, Guerra-Santos B, Silva RF (2015) Feeding behavior and the effect of photoperiod on the performance and hematological parameters of the pacamã catfish (Lophiosilurus alexandri). Appl Anim Behav Sci 171:211–218. https://doi.org/10.1016/j.applanim.2015.08.025 CrossRefGoogle Scholar
- Kraul S, Brittain K, Cantrell R, Nagao T, Ako H, Ogasawara A, Kitagawa H (1993) Nutritional factors affecting stress resistance in the larval mahi-mahi Coryphaena hippurus. J World Aquacult Soc 24:186–193. https://doi.org/10.1111/j.1749-7345.1993.tb00007.x CrossRefGoogle Scholar
- Mariano WS, Oba ET, Santos LRB, Fernandes MN (2009) Respostas fisiológicas de Jeju (Hoplerythrinus unitaeniatus) expostos ao ar atmosférico. Rev Bras Saúde Prod Anim 10:210–223 http://revistas.ufba.br/index.php/rbspa/article/view/1204/776 Google Scholar
- Melillo-Filho R, Takata R, Santos AEH, Silva WS, Ikeda AL, Rodrigues LA, Santos JCE, Salaro AL, Luz RK (2014) Draining system and feeding rate during the initial development of Lophiosilurus alexandri (Steindachner, 1877), a carnivorous freshwater fish. Aquac Res 45:1913–1920. https://doi.org/10.1111/are.12139 CrossRefGoogle Scholar
- Melo KDM, Oliveira GR, Brito TS, Soares DRP, Tessitore AJA, Alvarenga ER, Turra EM, Silva FCO, Teixeira EA (2016) Digestibilidade de ingredientes em dietas para juvenis de pacamã (Lophiosilurus alexandri). Pesq Agropec Bras 51:785–788. https://doi.org/10.1590/S0100-204X2016000600012 CrossRefGoogle Scholar
- Paital BJ (2014) Modulation of redox regulatory molecules and electron transport chain activity in muscle of air breathing fish Heteropneustes fossilis under air exposure stress. Comp Physiol B 184:65–76 https://doi-org.ez27.periodicos.capes.gov.br/10.1007/s00360-013-0778-8 CrossRefGoogle Scholar
- Pickering AD, Pottinger TG, Christie P (1982) Recovery of the brown trout, Salmo trutta L., from acute handling stress: a time-course study. J Fish Biol 20:229–244. https://doi.org/10.1111/j.1095-8649.1982.tb03923.x CrossRefGoogle Scholar
- Pottinger TG, Carrick TR, Yeomans WE (2002) The three-spined stickleback as an environmental sentinel: effects of stressors on whole-body physiological indices. J Fish Biol 61:207–229. https://doi.org/10.1111/j.1095-8649.2002.tb01747.x CrossRefGoogle Scholar
- Robertson L, Thomas P, Arnold CR, Trant JM (1987) Plasma cortisol and secondary stress responses of red drum to handling transport, rearing density, and a disease outbreak. Progress Fish Cult 49:1–12. https://doi.org/10.1577/1548-8640(1987)49<1:PCASSR>2.0.CO;2 CrossRefGoogle Scholar
- Salaro AL, Oliveira-Junior JCD, Lima FW, Ferraz RB, Pontes MD, Campelo DAV, Zuanon JAS, Luz RK (2015) Gelatin in replacement of bovine heart in feed training of Lophiosilurus alexandri in different water salinities. An Acad Bras Ciênc 87:2281–2287. https://doi.org/10.1590/0001-3765201520140575 CrossRefGoogle Scholar
- Tahmasebi-Kohyani A, Keyvanshokooh S, Nematollahi A, Mahmoudi N, Pasha-Zanoosi H (2012) Effects of dietary nucleotides supplementation on rainbow trout (Oncorhynchus mykiss) performance and acute stress response. Fish Physiol Biochem 38:431–440. https://doi.org/10.1007/s10695-011-9524-x CrossRefGoogle Scholar
- Takata R, Silva WS, Costa DC, Melillo-Filho R, Luz RK (2014) Effect of water temperature and prey concentrations on initial development of Lophiosilurus alexandri Steindachner, 1876 (Siluriformes: Pseudopimelodidae), a freshwater fish. Neotrop Ichthyol 12:853–859. https://doi.org/10.1590/1982-0224-20140063 CrossRefGoogle Scholar