Fish Physiology and Biochemistry

, Volume 45, Issue 1, pp 427–437 | Cite as

Molecular cloning, expression analysis, and the immune-related role of a thymosin β in the goldfish, Carassius auratus

  • Wenbo ChenEmail author
  • Fangfang Yan
  • Shaozong Qin
  • Haiyan DongEmail author


β-Thymosins play critical roles in the regulation of many important physiological processes, but their function in teleost fishes remains poorly understood. In this study, the full-length cDNA coding for a thymosin β (Tβ) was cloned and identified in goldfish, Carassius auratus (gfTβ). The gfTβ cDNA consisted of 653 bp with an open reading frame of 135 bp that encodes a 44 amino acid polypeptide. Sequence analysis revealed one thymosin domain and a highly conserved actin-binding motif (18LKKTET23). Expression of gfTβ transcript was detected ubiquitously in all tissues examined, with relatively higher levels in the brain, intestine, spleen, gill, skin, kidney, and testis. Cadmium and H2O2 exposure induced increases in gfTβ transcript levels in the liver and spleen. Moreover, gfTβ transcription was upregulated in response to LPS challenge in the spleen while Poly I:C treatment did not affect gfTβ expression. In vivo injection of recombinant gfTβ generated from an Escherichia coli system induced expression of T lymphocyte-related genes (RAG1 and CD8α). These results suggest that gfTβ may be involved in the immune response of teleost fishes via modulation of T lymphocyte development.


Thymosin β Cloning Immune response Cadmium Oxidative stress Carassius auratus 



This work is financially supported by the National Natural Science Foundation of China (No. 31101878), the Excellent Youth Foundation of Henan Polytechnic University (No. J2014-02), and the Key Science Research Project in University of Henan Province (No. 16A240001).

Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflicts of interest.

Supplementary material

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ESM 1 (DOCX 24.1 kb)
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Copyright information

© Springer Nature B.V. 2018

Authors and Affiliations

  1. 1.Department of Biology, Institute of Resources and EnvironmentHenan Polytechnic UniversityJiaozuoChina
  2. 2.Department of MedicineHuzhou UniversityHuzhouChina

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