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Fish Physiology and Biochemistry

, Volume 45, Issue 1, pp 401–415 | Cite as

Glucose-6-phosphate dehydrogenase in blunt snout bream Megalobrama amblycephala: molecular characterization, tissue distribution, and the responsiveness to dietary carbohydrate levels

  • Guang-Zhen Jiang
  • Hua-Juan Shi
  • Chao Xu
  • Ding-Dong Zhang
  • Wen-Bin Liu
  • Xiang-Fei LiEmail author
Article
First Online:

Abstract

This study aimed to characterize the full-length cDNA of glucose-6-phosphate dehydrogenase (G6PD) from Megalobrama amblycephala with its responses to dietary carbohydrate levels characterized. The cDNA obtained covered 2768 bp with an open reading frame of 1572 bp. Sequence alignment and phylogenetic analysis revealed a high degree of conservation (77–97%) among most fish and other higher vertebrates. The highest transcription of G6PD was observed in kidney followed by liver, whereas relatively low abundance was detected in eye. Then, the transcriptions and activities of G6PD as well as lipid contents were determined in the liver, muscle, and the adipose tissue of fish fed two dietary carbohydrate levels (30 and 42%) for 12 weeks. Hepatic transcriptions of fatty acid synthetase (FAS), acetyl-CoA carboxylase α (ACCα), sterol regulatory element-binding protein-1 (SREBP1), and peroxisome proliferator-activated receptor γ (PPARγ) were also measured to corroborate the lipogenesis derived from carbohydrates. The G6PD expressions and activities in both liver and the adipose tissue as well as the lipid contents in whole-body, liver, and the adipose tissue all increased significantly after high-carbohydrate feeding. Hepatic transcriptions of FAS, ACCα, SREBP1, and PPARγ were also up-regulated remarkably by the intake of a high-carbohydrate diet. These results indicated that the G6PD of M. amblycephala shared a high similarity with that of other vertebrates. Its expressions and activities in tissues were both highly inducible by high-carbohydrate feeding, as also held true for the transcriptions of other enzymes and/or transcription factors involved in lipogenesis, evidencing an enhanced lipogenesis by high dietary carbohydrate levels.

Keywords

Glucose-6-phosphate dehydrogenase Gene cloning Transcriptional analysis Carbohydrate levels Megalobrama amblycephala 
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Notes

Funding information

This research was funded by the Earmarked Fund for China Agriculture Research System (CARS-45-14) and the Fundamental Research Funds for the Central Universities in China (KJQN201708).

Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflict of interest.

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© Springer Nature B.V. 2018

Authors and Affiliations

  1. 1.Key Laboratory of Aquaculture Nutrition and Feed Science of Jiangsu Province, College of Animal Science and TechnologyNanjing Agricultural UniversityNanjingPeople’s Republic of China

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