Fish Physiology and Biochemistry

, Volume 45, Issue 1, pp 299–309 | Cite as

Proteomic variation in metamorphosing Paralichthys olivaceus induced by exogenous thyroid hormone

  • Jie Yu
  • Yuanshuai Fu
  • Suping Liu
  • Zhiyi ShiEmail author


Thyroid hormone (TH) is essential for Paralichthys olivaceus metamorphosis. Exogenous TH treatment induces premature metamorphosis in P. olivaceus larvae and a series of studies have been conducted to identify thyroid hormone-regulated functional genes and microRNAs involved in the metamorphosis of P. olivaceus; however, the proteins involved in this process remain to be fully clarified. In this study, the differential proteomic responses of P. olivaceus larvae to exogenous TH treatment were examined using tandem mass tags (TMT) for quantitation labeling followed by liquid chromatography tandem mass spectrometry (LC-MS/MS). The expression levels of 629 cellular proteins were identified to be significantly affected by TH treatment. The reliability of our TMT-labeled LC-MS/MS analysis was verified by examining the mRNA and protein levels of four selected proteins using quantitative real-time reverse-transcription PCR and western blot analyses. The possible biological significance of these proteins was further investigated by Gene Ontology (GO) enrichment, Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment, and protein-protein interaction analyses. Notably, we identified and described five groups of proteins involved in different important life events that were significantly regulated by exogenous TH treatment. Our study provides an improved understanding of the molecular mechanisms by which TH regulates the metamorphosis of P. olivaceus.


Paralichthys olivaceus Metamorphosis Thyroid hormone TMT-labeled LC-MS/MS analysis Proteomics 



The authors thank Prof. Haijin Liu (Chinese Academy of Fishery Sciences) for providing the experimental fish.

Funding information

This work was supported by the National Natural Science Foundation of China (No. 41676138).

Compliance with ethical standards

All animal experiments complied with the Chinese Legislation and Shanghai Ocean University Review Committee for the use of animal subjects.

Supplementary material

10695_2018_562_MOESM1_ESM.doc (64 kb)
ESM 1 (DOC 63 kb)


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Copyright information

© Springer Nature B.V. 2018

Authors and Affiliations

  • Jie Yu
    • 1
    • 2
    • 3
  • Yuanshuai Fu
    • 1
    • 2
    • 3
  • Suping Liu
    • 1
    • 2
    • 3
  • Zhiyi Shi
    • 1
    • 2
    • 3
    Email author
  1. 1.Key Laboratory of Freshwater Aquatic Genetic Resources, Ministry of AgricultureShanghai Ocean UniversityShanghaiChina
  2. 2.Key Laboratory of Exploration and Utilization of Aquatic Genetic Resources, Ministry of EducationShanghai Ocean UniversityShanghaiChina
  3. 3.Shanghai Collaborative Innovation for Aquatic Animal Genetics and BreedingShanghai Ocean UniversityShanghaiChina

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