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Red drum Sciaenops ocellatus growth and expression of bile salt-dependent lipase in response to increasing dietary lipid supplementation

  • Mayra L. González-Félix
  • Delbert M. GatlinIII
  • Martin Perez-Velazquez
  • Ken Webb
  • Armando García-Ortega
  • Michael Hume
Article
  • 51 Downloads

Abstract

Sciaenops ocellatus has a long history in aquaculture and many difficulties associated with its commercial culture have been addressed and successfully resolved; nevertheless, further research in lipid nutrition could address more comprehensive questions on the way these nutrients are utilized. The purpose of this study was to evaluate S. ocellatus growth and lipase gene expression in response to increasing dietary lipid supplementation. Four experimental diets were formulated to provide 3, 10, 16, or 23% lipid using menhaden fish oil. Twenty juveniles (mean initial weight 2.3 ± 0.1 g) were stocked per aquaria in a recirculating system; each diet was assigned to three aquaria and fed to fish for 6 weeks. At the end of the study, fish fed 3% of dietary lipid were significantly (P < 0.0001) smaller and showed significantly lower feed efficiency, condition factor, hepatosomatic index, and intraperitoneal fat than fish fed the other diets, but no differences were observed among fish fed 10, 16, or 23% lipid. A straight broken-line regression model for thermal growth coefficient provided an estimated value of 9.4% of dietary lipid as the optimal inclusion level. The bile salt-dependent lipase (BSDL) of red drum was 80.3 kDa. Relative gene expression of BSDL was significantly higher (P = 0.0007) in fish fed 10% lipid, with no differences among the other dietary treatments. Results provided could help monitor the metabolic status of farmed fish and contribute to optimize diet formulations based on maximum gene expression of BSDL for supplementation of dietary lipid.

Keywords

Sciaenops ocellatus Bile salt-dependent lipase Gene expression Dietary lipid 

Notes

Acknowledgments

This research was partly supported by CONACYT (Consejo Nacional de Ciencia y Tecnologia), Mexico, for Drs. González-Félix (CONACYT Application 454912, Registry 312949) and Perez -Velazquez (CONACYT Application 454922, Registry 312944). The mention of trademarks or proprietary products does not constitute an endorsement of the product and does not imply its approval to the exclusion of other products that may also be suitable.

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Copyright information

© Springer Science+Business Media B.V., part of Springer Nature 2018

Authors and Affiliations

  • Mayra L. González-Félix
    • 1
  • Delbert M. GatlinIII
    • 2
  • Martin Perez-Velazquez
    • 1
  • Ken Webb
    • 3
  • Armando García-Ortega
    • 4
  • Michael Hume
    • 5
    • 6
  1. 1.Department of Scientific and Technological ResearchUniversity of SonoraHermosilloMexico
  2. 2.Department of Wildlife and Fisheries SciencesTexas A&M UniversityCollege StationUSA
  3. 3.Fisheries and Mariculture Laboratory, Marine Science InstituteThe University of Texas at AustinPort AransasUSA
  4. 4.College of Agriculture, Forestry and Natural Resource Management, Pacific Aquaculture and Coastal Resources CenterUniversity of Hawaii at HiloHiloUSA
  5. 5.USDA, Food and Feed Safety UnitAgricultural Research ServiceCollege StationUSA
  6. 6.Department of Poultry ScienceTexas A&M UniversityCollege StationUSA

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