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Fish Physiology and Biochemistry

, Volume 44, Issue 5, pp 1309–1318 | Cite as

Increased water temperature altered hemato-biochemical parameters and structure of peripheral erythrocytes in striped catfish Pangasianodon hypophthalmus

  • Md. ShahjahanEmail author
  • Md. Helal Uddin
  • Victor Bain
  • Md. Mahfuzul Haque
Article

Abstract

The increase of water temperature due to global warming is a great concern of aquaculturists and fishery biologists. In the present study, we examined the effects of high temperature on hematological parameters and blood glucose levels in striped catfish, Pangasianodon hypophthalmus exposed to three temperature conditions (28, 32, and 36 °C) for 7 days. Fish were sacrificed at days 1, 3, and 7. Erythroblasts (Ebs), erythrocytic cellular abnormalities (ECA), and erythrocytic nuclear abnormalities (ENA) were assayed using peripheral erythrocytes of the sampled fishes. Hemoglobin (Hb) and red blood cell (RBC) significantly (P < 0.05) decreased at 36 °C after 3 and 7 days of exposure, whereas white blood cell (WBC) showed opposite scenario. Blood glucose levels significantly (P < 0.05) increased at 36 °C on day 3. Frequencies of Ebs, ECA, and ENA were found to be elevated at increased temperature. Differential leucocytes count showed significant increases in neutrophil and decreases in lymphocytes in the highest temperature (36 °C). Dissolved oxygen decreased and free CO2 increased significantly (P < 0.05) with increasing temperature, while the pH and total alkalinity of the water were almost unchanged throughout the study period. Therefore, the present study demonstrated that striped catfish feel better adaptation at 28 and 32 °C, while high temperature 36 °C is likely stressful to this fish species.

Keywords

Striped catfish Temperature Hematological parameters Blood glucose Water quality parameters 

Notes

Acknowledgments

This study was supported by a grant (2017/282/BAU) from Bangladesh Agricultural University Research System to the corresponding author which is gratefully acknowledged.

Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflict of interests. The authors themselves are responsible for the content of the paper.

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Copyright information

© Springer Science+Business Media B.V., part of Springer Nature 2018

Authors and Affiliations

  • Md. Shahjahan
    • 1
    Email author
  • Md. Helal Uddin
    • 1
  • Victor Bain
    • 1
  • Md. Mahfuzul Haque
    • 1
  1. 1.Department of Fisheries ManagementBangladesh Agricultural UniversityMymensinghBangladesh

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