Fish Physiology and Biochemistry

, Volume 44, Issue 2, pp 693–702 | Cite as

PPARβ in yellow catfish Pelteobagrus fulvidraco: molecular characterization, tissue expression and transcriptional regulation by dietary Cu and Zn

  • Wen-Jing You
  • Xiao-Ying Tan
  • Guang-Hui Chen
  • Chuan-Chuan Wei
  • Dan-Dan Li
Article

Abstract

Peroxisome proliferator-activated receptor beta (PPARβ) is a ligand-activated transcription factor that plays critical roles in the regulation of many important physiological processes. In this study, PPARβ was cloned and characterized in yellow catfish Pelteobagrus fulvidraco. PPARβ cDNA was 2350 bp in length with an open reading frame (ORF) of 1530 bp, encoding 509 amino acids, a 5′-untranslated region (UTR) of 474 bp, and a 3′-UTR of 346 bp. Similar to mammals, PPARβ protein was predicted to consist of four domains, the A/B domain, DNA-binding domain (DBD), D domain, and ligand-binding domain (LBD). The DBD contained two zinc fingers with eight conserved cysteine residues. The predicted secondary structure of LBD consisted of 12 highly conserved α-helices and a small β-sheet of 4 strands. In addition, PPARβ was widely expressed across the tested tissues (liver, heart, muscle, intestine, brain, spleen, kidney, fat, ovary, and gill), but at the variable levels. Furthermore, the transcriptional responses of PPARβ by dietary Cu and Zn levels were also investigated. Dietary Cu levels showed no significant effects on PPARβ mRNA levels in the liver and intestine; in contrast, dietary Zn levels upregulated the hepatic PPARβ mRNA levels, but not in the intestine. The present study serves to increase our understanding into the function of the PPARβ gene in fish.

Keywords

Pelteobagrus fulvidraco PPARβ Molecular characterization Tissue expression Transcriptional regulation Cu Zn 

Abbreviations

Cu

Copper

DBD

the DNA-binding domain

GAPDH

Glyceraldehyde-3-phosphate dehydrogenase

HPRT

Hypoxanthine-guanine phosphoribosyl transferase

LBD

the ligand-binding domain

MS-222

Tricaine methanesulfonate

ORF

Open reading frame

PPARβ

Peroxisome proliferators-activated receptor beta

PPRE

Peroxisome proliferator response elements

RPL7

Ribosomal protein L7

RXR

Retinoic X receptor

UTR

Untranslated region

Zn

Zinc

Notes

Acknowledgements

This work was supported by the National Natural Science Foundation of China (NSFC, grant no.: 31572605) and by the Scientific Research Foundation for the Returned Overseas Chinese Scholars of Ministry of Education of China.

Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflicts of interest.

Supplementary material

10695_2018_465_MOESM1_ESM.docx (14 kb)
ESM 1 (DOCX 14 kb)

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Copyright information

© Springer Science+Business Media B.V., part of Springer Nature 2018

Authors and Affiliations

  1. 1.Key Laboratory of Freshwater Animal Breeding, Ministry of Agriculture of P.R.C., Fishery CollegeHuazhong Agricultural UniversityWuhanChina

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