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Fish Physiology and Biochemistry

, Volume 43, Issue 1, pp 127–136 | Cite as

Nucleic acids digestion by enzymes in the stomach of snakehead (Channa argus) and banded grouper (Epinephelus awoara)

  • Yu Liu
  • Yanfang Zhang
  • Wei Jiang
  • Jing Wang
  • Xiaoming Pan
  • Wei Wu
  • Minjie Cao
  • Ping DongEmail author
  • Xingguo LiangEmail author
Article

Abstract

Dietary nucleic acids (NAs) were important nutrients. However, the digestion of NAs in stomach has not been studied. In this study, the digestion of NAs by enzymes from fish stomach was investigated. The snakehead pepsins (SP) which were the main enzymes in stomach were extracted and purified. The purity of SP was evaluated by SDS-PAGE and HPLC. The snakehead pepsin 2 (SP2) which was the main component in the extracts was used for investigating the protein and NAs digestion activity. SP2 could digest NAs, including λ DNA and salmon sperm DNA. Interestingly, the digestion could be inhibited by treatment of alkaline solution at pH 8.0 and pepstatin A, and the digestion could happen either in the presence or absence of hemoglobin (Hb) and BSA as the protein substrates. Similarly, the stomach enzymes of banded grouper also showed the NAs digestion activity. NAs could be digested by the stomach enzymes of snakehead and banded grouper. It may be helpful for understanding both animal nutrition and NAs metabolic pathway.

Keywords

Fish External nucleic acids Feed Pepsin Stomach 

Abbreviations

NAs

Nucleic acids

SP

Snakehead pepsin

SP2

Snakehead pepsin 2

SSE

Snakehead stomach enzymes

BSE

Bovine stomach enzymes

Hb

Hemoglobin

ddH2O

Double distilled water

Notes

Acknowledgments

This work was supported by National Natural Science Foundation of China 31201327 (to P.D.), by support from National Youth Qianren Plan of China (to X.L.) and from the Scientific Research Foundation for the Returned Overseas Chinese Scholars, State Education Ministry (to P.D.).

Supplementary material

10695_2016_273_MOESM1_ESM.docx (92 kb)
Supplementary material 1 (DOCX 92 kb)

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Copyright information

© Springer Science+Business Media Dordrecht 2016

Authors and Affiliations

  • Yu Liu
    • 1
    • 2
  • Yanfang Zhang
    • 1
  • Wei Jiang
    • 2
  • Jing Wang
    • 1
  • Xiaoming Pan
    • 1
  • Wei Wu
    • 1
  • Minjie Cao
    • 3
    • 4
  • Ping Dong
    • 1
    Email author
  • Xingguo Liang
    • 1
    Email author
  1. 1.College of Food Science and EngineeringOcean University of ChinaQingdaoChina
  2. 2.Innovation and Application InstituteZhejiang Ocean UniversityZhoushanChina
  3. 3.College of Biological EngineeringJimei UniversityXiamenChina
  4. 4.Key Laboratory of Science and Technology for Aquaculture and Food SafetyJimei UniversityXiamenChina

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