Fish Physiology and Biochemistry

, Volume 43, Issue 4, pp 913–922 | Cite as

Effects of dietary vitamin E supplementation on growth performance, fatty acid composition, lipid peroxidation and peroxisome proliferator-activated receptors (PPAR) expressions in juvenile blunt snout bream Megalobrama amblycephala

  • Yin Zhang
  • Yang Li
  • Xiao Liang
  • Jian GaoEmail author


A 9-week feeding experiment was conducted to evaluate the effects of dietary vitamin E (VE) supplementation on growth performance, liver fatty acid composition, lipid peroxidation and peroxisome proliferator-activated receptors (PPAR) genes expressions in blunt snout bream juveniles. Fish (average initial weight: 0.59 g) were fed diet supplemented with 0, 50, 100, 300 and 500 mg α-tocopherol acetate/kg in triplicates, which were found to, respectively, contain 11.2, 56.3, 114.6, 306.5 and 588.4 mg α-tocopherol/kg diet. Results showed that final weight, body weight gain and specific growth rate significantly increased with increasing dietary VE supplemented level from 11.2 to 56.3 mg/kg. When the broken-line model was employed to estimate the adequate requirement of vitamin E based on body weight gain, the optimal level was 55.5 mg/kg in diet. Hepatosomatic index value significantly decreased with incremental dietary VE levels. However, liver VE concentration showed a direct relationship with the dietary VE level. The percentages of 20:5n-3, 22:6n-3 and total n-3 long chain polyunsaturated fatty acids in liver increased with increasing dietary VE supplementation. Meanwhile, the expressions of PPAR-α, PPAR-β and PPAR-γ in liver were down-regulated by supplementation of dietary VE level from 56.3 to 588.4 mg/kg. In conclusion, supplementation of more than 55.5 mg/kg vitamin E may improve growth and increase n-3 LC-PUFA content in blunt snout bream, which is beneficial to human consumer.


Megalobrama amblycephala Vitamin E Fatty acid composition Lipid oxidation PPAR expressions 



This study was supported by the Fundamental Research Funds for the Central Universities of China (Project 2013PY074).


  1. A.O.A.C (1990) Official methods of analysis of the association of official analytical chemists, 15th edn. Association of Official Analytical Chemists, ArlingtonGoogle Scholar
  2. Bae JY, Park GH, Yoo KY, Lee JY, Kim DJ, Bai SC (2013) Evaluation of optimum dietary vitamin E requirements using DL-alpha-tocopheryl acetate in the juvenile eel, Anguilla japonica. J Appl Ichthyol 29:213–217CrossRefGoogle Scholar
  3. Bai SC, Gatlin DM (1993) Dietary vitamin-E concentration and duration of feeding affect tissue alpha-tocopherol concentrations of channel catfish (Ictalurus punctatus). Aquaculture 113:129–135CrossRefGoogle Scholar
  4. Baker RTM, Davies SJ (1996) Oxidative nutritional stress associated with feeding rancid oils to African catfish, Clarias gariepinus (Burchell) and the protective role of α-tocopherol. Aquac Res 27:795–803CrossRefGoogle Scholar
  5. Bligh EG, Dyer WJ (1959) A rapid method for total lipid extraction and purification. Can J Biochem Physiol 37:911–917CrossRefPubMedGoogle Scholar
  6. Chen HH, David AH, Shannon KB, Robert HD (2004) Effect of dietary vitamin E on growth, tissue lipid peroxidation, and erythrocyte fragility of transgenic coho salmon, Oncorhynchus kisutch. Comp Biochem Physiol A 139:199–204CrossRefGoogle Scholar
  7. Cowey CB, Adron JW, Youngson A (1983) The vitamin E requirement of rainbow trout (Salmo gairdneri) given diets containing polyunsaturated fatty acids derived from fish oil. Aquaculture 30:85–93CrossRefGoogle Scholar
  8. Dressel U, Allen TL, Pippal JB, Rohde PR, Lau P, Muscat GE (2003) The peroxisome proliferator-activated receptor beta/delta agonist, GW501516, regulates the expression of genes involved in lipid catabolism and energy uncoupling in skeletal muscle cells. Mol Endocrinol 17:2477–2493CrossRefPubMedGoogle Scholar
  9. Fang F, Kang ZF, Wong CW (2010) Vitamin E tocotrienols improve insulin sensitivity through activating peroxisome proliferator-activated receptors. Mol Nutr Food Res 54:345–352CrossRefPubMedGoogle Scholar
  10. Gao J, Koshio S, Ishikawa M, Yokoyama S, Ren T, Komilus CF, Han Y (2012a) Effects of dietary palm oil supplements with oxidized and non-oxidized fish oil on growth performances and fatty acid compositions of juvenile Japanese sea bass, Laterolabrax japonicas. Aquaculture 324–325:97–103CrossRefGoogle Scholar
  11. Gao J, Koshio S, Ishikawa M, Yokoyama S, Mamauag REP, Han Y (2012b) Effects of dietary oxidized fish oil with vitamin E supplementation on growth performance and reduction of lipid peroxidation in tissues and blood of red sea bream Pagrus major. Aquaculture 356–357:73–79CrossRefGoogle Scholar
  12. Hamre K (2011) Metabolism, interaction, requirements and functions of vitamin E in fish. Aquac Nutr 17:98–115CrossRefGoogle Scholar
  13. Hamre K, Lie Ø (1995) α-Tocopherol levels in different organs of Atlantic salmon (Salmo salar L.)—Effect of smoltification, dietary levels of polyunsaturated fatty acids and vitamin E. Comp Biochem Physiol A 111:547–554CrossRefGoogle Scholar
  14. Hua XN, Yu DJ, Peng H, Zhen M, Ji LL (2014) Effect of dietary vitamin E on the growth performance and nonspecific immunity in sub-adult turbot (Scophthalmus maximus). Fish Shellfish Immunol 41:501–506CrossRefGoogle Scholar
  15. Jump DB (2002) Dietary polyunsaturated fatty acids and regulation of gene transcription. Curr Opin Lipidol 13:155–164CrossRefPubMedGoogle Scholar
  16. Jumroensri P, Kiront V, Satoh S, Watanabe T (2005) Antioxidant defense of rainbow trout (Oncorhynchus mykiss) in relation to dietary n-3 highly unsaturated fatty acids and vitamin E contents. Comp Biochem Physiol C 140:187–196CrossRefGoogle Scholar
  17. Kliewer SA, Xu HE, Lambert MH, Willson TM (2001) Peroxisome proliferator-activated receptors from genes to physiology. Recent Prog Horm Res 56:239–265CrossRefPubMedGoogle Scholar
  18. Kuenzli S, Saurat JH (2003) Peroxisome proliferator-activated receptors in cutaneous biology. Br J Dermatol 149:229–236CrossRefPubMedGoogle Scholar
  19. Lebold KM, Jump DB, Miller GW, Wright CL, Labut EM, Barton CL, Tanguay RL, Traber MG (2011) Vitamin E deficiency decreases long-chain PUFA in Zebrafish (Danio rerio). J Nutr 141:2113–2118CrossRefPubMedPubMedCentralGoogle Scholar
  20. Lee CH, Olson P, Evans RM (2003) Minireview: lipid metabolism, metabolic diseases, and peroxisome proliferator-activated receptors. Endocrinology 144:2201–2207CrossRefPubMedGoogle Scholar
  21. Li P, Gatlin DM (2009) Dietary vitamin E requirement of the red drum Sciaenops ocellatus. Aquac Nutr 15:313–319CrossRefGoogle Scholar
  22. Li Y, Gao J, Huang S (2015) Effects of different dietary phospholipid levels on growth performance, fatty acid composition, PPAR gene expressions and antioxidant responses of blunt snout bream Megalobrama amblycephala fingerlings. Fish Physiol Biochem 41:423–436CrossRefPubMedGoogle Scholar
  23. Lim C, Yildirim-Aksoy M, Shelby R, Li MH, Klesius PH (2010) Growth performance, vitamin E status, and proximate and fatty acid composition of channel catfish, Ictalurus punctatus, fed diets containing various levels of fish oil and vitamin E. Fish Physiol Biochem 36:855–866CrossRefPubMedGoogle Scholar
  24. Lin YH, Shiau SY (2005) Dietary vitamin E requirement for grouper, Epinephelus malabaricus, at two lipid levels, and their effects on immune responses. Aquaculture 248:235–244CrossRefGoogle Scholar
  25. Liu B, Xu P, Xie J, Ge XP, Xia SL, Song CY, Zhou QL, Miao LH, Ren MC, Pan LK, Chen RL (2014) Effects of emodin and vitamin E on the growth and crowding stress of Wuchang bream (Megalobrama amblycephala). Fish Shellfish Immunol 40:595–602CrossRefPubMedGoogle Scholar
  26. Lu KL, Xu WN, Li XF, Liu WB, Wang LN, Zhang CN (2013) Hepatic triacylglycerol secretion, lipid transport and tissue lipid uptake in blunt snout bream (Megalobrama amblycephala) fed high-fat diet. Aquaculture 408–409:160–168CrossRefGoogle Scholar
  27. Mourente G, Diaz-Salvago E, Tocher DR, Bell JG (2000) Effects of dietary polyunsaturated fatty acid/vitamin E (PUFA/tocopherol) ratio on antioxidant defence mechanisms of juvenile gilthead sea bream (Sparus aurata L, Osteichthyes, Sparidae). Fish Physiol Biochem 23:337–351CrossRefGoogle Scholar
  28. Mourente G, Diaz-Salvago E, Bell JG, Tocher DR (2002) Increased activities of hepatic antioxidant defence enzymes in juvenile gilthead sea bream (Sparus aurata L.) fed dietary oxidised oil: attenuation by dietary vitamin E. Aquaculture 214:343–361CrossRefGoogle Scholar
  29. Mourente G, Bell JG, Tocher DR (2007) Does dietary tocopherol level affect fatty acid metabolism in fish? Fish Physiol Biochem 33:269–280CrossRefGoogle Scholar
  30. Qi C, Zhu Y, Reddy JK (2000) Peroxisome proliferator-activated receptors, coactivators, and downstream targets. Cell Biochem Biophys 32:187–204CrossRefPubMedGoogle Scholar
  31. Robbins KR (1986) A method, SAS program, and example for fitting the broken line to growth data. University of Tennesse Agricultural Experiment Station Research Report. University of Tennesse, KnoxvilleGoogle Scholar
  32. Ruyter B, Andersen O, Dehli A, Ostlund Farrants AK, Gjoen T, Thomassen MS (1997) Peroxisome proliferator activated receptors in Atlantic salmon (Salmo salar): effects on PPAR transcription and acyl-CoA oxidase activity in hepatocytes by peroxisome proliferators and fatty acids. Biochim Biophys Acta 1348:331–338CrossRefPubMedGoogle Scholar
  33. Sau SK, Paul BN, Mohanta KN, Mohanty SN (2004) Dietary vitamin E requirement, fish performance and carcass composition of rohu (Labeo rohita) fry. Aquaculture 240:359–368CrossRefGoogle Scholar
  34. Stumvoll M, Haring H (2002) The peroxisome proliferator-activated receptor-gamma 2 pro12Ala polymorphism. Diabetes 51:2341–2347CrossRefPubMedGoogle Scholar
  35. Tocher DR, Mourente G, Vander Eecken A, Evjemo JO, Diaz E, Wille M, Bell JG, Olsen Y (2003) Comparative study of antioxidant defence mechanisms in marine fish fed variable levels of oxidised oil and vitamin E. Aquacult Int 11:195–216CrossRefGoogle Scholar
  36. Wilson RP, Bowser PR, Poe WE (1984) Dietary vitamin E requirement of fingerling channel catfish. J Nutr 114:2053–2058PubMedGoogle Scholar
  37. Yagi K (1987) Lipid peroxides and human disease. Chem Phys Lipids 45:337–351CrossRefPubMedGoogle Scholar
  38. Yang L, Xiao L, Yin Z, Jian G (2016) Effects of different dietary soybean oil levels on growth, lipid deposition, tissues fatty acid composition and hepatic lipid metabolism related gene expressions in blunt snout bream (Megalobrama amblycephala) juvenile. Aquaculture 451:16–23CrossRefGoogle Scholar
  39. Yong KZ, Ting TH, Yong HW, Lei P, Xiao JC, Wei MW, Jian G (2014) Effects of vitamin E supplementation on growth, lipid peroxidation and fatty acid composition of Dojo loach (Misgurnus anguillicaudatus Cantor) fingerlings. Aquac Res. doi: 10.1111/are.12612 Google Scholar
  40. Zhou M, Liu B, Ge XP, Xie J, Chen RL, Cui YT, Wan JJ, Michael HH, Habte-Tsion MH (2013a) Effects of vitamin E on serum biochemical indexes and antioxidant capacity of Megalobrama amblycephala under acute high temperature stress and recovery. J Fish China 37:1369–1377 (in Chinese) CrossRefGoogle Scholar
  41. Zhou M, Liu B, Ge XP, Xie J, Wan JJ, Cui SL (2013b) Effects of vitamin E on growth, physiological and biochemical indexes of blood and muscle physiochemical indices in Wuchang bream Megalobrama amblycephala. Chin J Anim Nutr 25:1488–1496 (in Chinese) Google Scholar
  42. Zhou QC, Wang LG, Wang HL, Wang T, Elmada CZ, Xie FJ (2013c) Dietary vitamin E could improve growth performance, lipid peroxidation and non-specific immune responses for juvenile cobia (Rachycentron canadum). Aquac Nutr 19:421–429CrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media Dordrecht 2017

Authors and Affiliations

  1. 1.College of Fisheries, Key Lab of Agricultural Animal Genetics, Breeding and Reproduction of Ministry of Education/Key Lab of Freshwater Animal Breeding, Ministry of AgricultureHuazhong Agricultural UniversityWuhanChina

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