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Fish Physiology and Biochemistry

, Volume 43, Issue 4, pp 913–922 | Cite as

Effects of dietary vitamin E supplementation on growth performance, fatty acid composition, lipid peroxidation and peroxisome proliferator-activated receptors (PPAR) expressions in juvenile blunt snout bream Megalobrama amblycephala

  • Yin Zhang
  • Yang Li
  • Xiao Liang
  • Jian GaoEmail author
Article

Abstract

A 9-week feeding experiment was conducted to evaluate the effects of dietary vitamin E (VE) supplementation on growth performance, liver fatty acid composition, lipid peroxidation and peroxisome proliferator-activated receptors (PPAR) genes expressions in blunt snout bream juveniles. Fish (average initial weight: 0.59 g) were fed diet supplemented with 0, 50, 100, 300 and 500 mg α-tocopherol acetate/kg in triplicates, which were found to, respectively, contain 11.2, 56.3, 114.6, 306.5 and 588.4 mg α-tocopherol/kg diet. Results showed that final weight, body weight gain and specific growth rate significantly increased with increasing dietary VE supplemented level from 11.2 to 56.3 mg/kg. When the broken-line model was employed to estimate the adequate requirement of vitamin E based on body weight gain, the optimal level was 55.5 mg/kg in diet. Hepatosomatic index value significantly decreased with incremental dietary VE levels. However, liver VE concentration showed a direct relationship with the dietary VE level. The percentages of 20:5n-3, 22:6n-3 and total n-3 long chain polyunsaturated fatty acids in liver increased with increasing dietary VE supplementation. Meanwhile, the expressions of PPAR-α, PPAR-β and PPAR-γ in liver were down-regulated by supplementation of dietary VE level from 56.3 to 588.4 mg/kg. In conclusion, supplementation of more than 55.5 mg/kg vitamin E may improve growth and increase n-3 LC-PUFA content in blunt snout bream, which is beneficial to human consumer.

Keywords

Megalobrama amblycephala Vitamin E Fatty acid composition Lipid oxidation PPAR expressions 

Notes

Acknowledgments

This study was supported by the Fundamental Research Funds for the Central Universities of China (Project 2013PY074).

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Copyright information

© Springer Science+Business Media Dordrecht 2017

Authors and Affiliations

  1. 1.College of Fisheries, Key Lab of Agricultural Animal Genetics, Breeding and Reproduction of Ministry of Education/Key Lab of Freshwater Animal Breeding, Ministry of AgricultureHuazhong Agricultural UniversityWuhanChina

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