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Fish Physiology and Biochemistry

, Volume 42, Issue 1, pp 389–401 | Cite as

Acyl-coenzyme A oxidases 1 and 3 in brown trout (Salmo trutta f. fario): Can peroxisomal fatty acid β-oxidation be regulated by estrogen signaling?

  • Tânia Vieira MadureiraEmail author
  • L. Filipe C. Castro
  • Eduardo Rocha
Article

Abstract

Acyl-coenzyme A oxidases 1 (Acox1) and 3 (Acox3) are key enzymes in the regulation of lipid homeostasis. Endogenous and exogenous factors can disrupt their normal expression/activity. This study presents for the first time the isolation and characterization of Acox1 and Acox3 in brown trout (Salmo trutta f. fario). Additionally, as previous data point to the existence of a cross-talk between two nuclear receptors, namely peroxisome proliferator-activated receptors and estrogen receptors, it was here evaluated after in vitro exposures of trout hepatocytes the interference caused by ethynylestradiol in the mRNA levels of an inducible (by peroxisome proliferators) and a non-inducible oxidase. The isolated Acox1 and Acox3 show high levels of sequence conservation compared to those of other teleosts. Additionally, it was found that Acox1 has two alternative splicing isoforms, corresponding to 3I and 3II isoforms of exon 3 splicing variants. Both isoforms display tissue specificity, with Acox1–3II presenting a more ubiquitous expression in comparison with Acox1–3I. Acox3 was expressed in almost all brown trout tissues. According to real-time PCR data, the highest estrogenic stimulus was able to cause a down-regulation of Acox1 and an up-regulation of Acox3. So, for Acox1 we found a negative association between an estrogenic input and a directly activated PPARα target gene. In conclusion, changes in hormonal estrogenic stimulus may impact the mobilization of hepatic lipids to the gonads, with ultimate consequences in reproduction. Further studies using in vivo assays will be fundamental to clarify these issues.

Keywords

Acox1 Acox3 PPARs Estrogens Brown trout 

Notes

Acknowledgments

Study supported by the ERDF—European Regional Development Fund, through the COMPETE—Operational Competitiveness Programme, and by National Public Funds through FCT—Foundation for Science and Technology, primarily under Project “PTDC/CVT/115618/2009,” post-doc Grant [SFRH/BPD/97139/2013 to T.V.M.] and additionally by Project “UID/Multi/04423/2013.”

Compliance with ethical standards

Conflict of interest

The authors declare that they have no conflict of interest.

Supplementary material

10695_2015_146_MOESM1_ESM.docx (17 kb)
Table 3 Identity and similarity analysis for Acox1 and Acox3 protein sequences (DOCX 18 kb)

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Copyright information

© Springer Science+Business Media Dordrecht 2015

Authors and Affiliations

  • Tânia Vieira Madureira
    • 1
    • 2
    Email author
  • L. Filipe C. Castro
    • 1
    • 3
  • Eduardo Rocha
    • 1
    • 2
  1. 1.CIIMAR – Interdisciplinary Centre of Marine and Environmental ResearchU.Porto – University of PortoPortoPortugal
  2. 2.Laboratory of Histology and Embryology, Department of Microscopy, ICBAS – Institute of Biomedical Sciences Abel SalazarU.Porto – University of PortoPortoPortugal
  3. 3.Department of Biology, FCUP – Faculty of SciencesU.Porto – University of PortoPortoPortugal

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