Ontogeny and kinetics of carnitine palmitoyltransferase I in hepatopancreas and skeletal muscle of grass carp (Ctenopharyngodon idella)
- 195 Downloads
The ontogeny and kinetics of carnitine palmitoyltransferase I (CPT I) were investigated in hepatopancreas and muscle throughout four developmental stages (newly hatched larvae, 1-month-old juvenile, 3-month-old, and 6-month-old, respectively) of grass carp Ctenopharyngodon idella. In hepatopancreas, the maximal velocity (V max) significantly increased from hatching to 1-month-old grass carp and then gradually declined at 6-month-old grass carp. In muscle, CPT I activity was the highest at 1-month-old grass carp, nearly twofold higher than that at hatching (P < 0.05). The Michaelis constant (K m) value was also the highest for 1-month-old in both tested tissues. Carnitine concentrations (FC, AC and TC) were the lowest for 3-month-old grass carp and remained relatively constant in both tissues from fish under the other developmental stages. The FC concentration in hepatopancreas and muscle at four developmental stages were less than the respective K m, indicating that grass carp required supplemental carnitine in their food to ensure that CPT I activity was not constrained by carnitine availability.
KeywordsCtenopharyngodon idella Carnitine CPT I Kinetics Development
One-way analysis of variance
5, 5′-dithio-bis-(2-nitrobenzoic acid)
- MS 222
Standard error of mean
Maximal reaction velocity
This work was funded by ‘973’ project, China (Grant No. 2009CB118706), and the Special Fund for Agro-Scientific Research in the Public Interest of China (Grant No. 201003020).
- FAO (2012) The state of world fisheries and aquaculture 2012. RomeGoogle Scholar
- Lin X, Odle J (1995) Regulation of fatty acid oxidation by hepatic mitochondria from neonatal pigs via carnitine palmitoyltransferase I. J Anim Sci 73(Suppl 1):77Google Scholar
- McGarry J, Mills S, Long C, Foster D (1983) Observations on the affinity for carnitine, and malonyl-CoA sensitivity, of carnitine palmitoyltransferase I in animal and human tissues: demonstration of the presence of malonyl-CoA in non-hepatic tissues of the rat. Biochem J 214:21–28PubMedCentralCrossRefPubMedGoogle Scholar
- Peffer PL, Lin X, Jacobi SK, Gatlin LA, Woodworth J, Odle J (2007) Ontogeny of carnitine palmitoyltransferase I activity, carnitine-Km, and mRNA abundance in pigs throughout growth and development. J Nutr 137:898–903Google Scholar