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Fish Physiology and Biochemistry

, Volume 41, Issue 6, pp 1393–1401 | Cite as

Ontogeny and kinetics of carnitine palmitoyltransferase I in hepatopancreas and skeletal muscle of grass carp (Ctenopharyngodon idella)

  • Wei Hu
  • Zhi LuoEmail author
  • Kang-Sen Mai
  • Cai-Xia Liu
  • Jia-Lang Zheng
Article
  • 195 Downloads

Abstract

The ontogeny and kinetics of carnitine palmitoyltransferase I (CPT I) were investigated in hepatopancreas and muscle throughout four developmental stages (newly hatched larvae, 1-month-old juvenile, 3-month-old, and 6-month-old, respectively) of grass carp Ctenopharyngodon idella. In hepatopancreas, the maximal velocity (V max) significantly increased from hatching to 1-month-old grass carp and then gradually declined at 6-month-old grass carp. In muscle, CPT I activity was the highest at 1-month-old grass carp, nearly twofold higher than that at hatching (P < 0.05). The Michaelis constant (K m) value was also the highest for 1-month-old in both tested tissues. Carnitine concentrations (FC, AC and TC) were the lowest for 3-month-old grass carp and remained relatively constant in both tissues from fish under the other developmental stages. The FC concentration in hepatopancreas and muscle at four developmental stages were less than the respective K m, indicating that grass carp required supplemental carnitine in their food to ensure that CPT I activity was not constrained by carnitine availability.

Keywords

Ctenopharyngodon idella Carnitine CPT I Kinetics Development 

Abbreviations

AC

Acyl carnitine

TC

Total carnitine

FC

Free carnitine

ANOVA

One-way analysis of variance

CPT

Carnitine palmitoyltransferase

DTNB

5, 5′-dithio-bis-(2-nitrobenzoic acid)

Km

Michaelis–Menten constants

MS 222

Tricaine methanesulfonate

SEM

Standard error of mean

Vmax

Maximal reaction velocity

Notes

Acknowledgments

This work was funded by ‘973’ project, China (Grant No. 2009CB118706), and the Special Fund for Agro-Scientific Research in the Public Interest of China (Grant No. 201003020).

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Copyright information

© Springer Science+Business Media Dordrecht 2015

Authors and Affiliations

  • Wei Hu
    • 1
    • 2
  • Zhi Luo
    • 1
    • 2
    Email author
  • Kang-Sen Mai
    • 3
  • Cai-Xia Liu
    • 1
    • 2
    • 4
  • Jia-Lang Zheng
    • 1
    • 2
    • 5
  1. 1.Key Laboratory of Freshwater Animal Breeding, Ministry of Agriculture of P.R.C., Fishery CollegeHuazhong Agricultural UniversityWuhanPeople’s Republic of China
  2. 2.Freshwater Aquaculture Collaborative Innovative Centre of Hubei ProvinceWuhanPeople’s Republic of China
  3. 3.College of FisheriesOcean University of ChinaQingdaoPeople’s Republic of China
  4. 4.Bureau of Aquatic Products of Xiantao CityXiantaoPeople’s Republic of China
  5. 5.National Engineering Research Center of Marine Facilities AquacultureZhejiang Ocean UniversityZhoushanPeople’s Republic of China

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