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Fish Physiology and Biochemistry

, Volume 41, Issue 2, pp 449–461 | Cite as

Molecular cloning and functional characterization of the hepcidin gene from the convict cichlid (Amatitlania nigrofasciata) and its expression pattern in response to lipopolysaccharide challenge

  • Jing-Ruei Chi
  • Long-Si Liao
  • Rong-Guang Wang
  • Chu-Sian Jhu
  • Jen-Leih WuEmail author
  • Shao-Yang HuEmail author
Article

Abstract

The hepcidin gene is widely expressed in many fish species and functions as an antimicrobial peptide, suggesting that it plays an important role in the innate immune system of fish. In the present study, the Amatitlania nigrofasciata hepcidin gene (AN-hepc) was cloned from the liver and its expression during an immune response was characterized. The results of quantitative PCR and RT-PCR showed that the AN-hepc transcript was most abundant in the liver. The expression of AN-hepc mRNA was significantly increased in the liver, stomach, heart, intestine, gill and muscle but was not significantly altered in the spleen, kidney, brain or skin after lipopolysaccharide challenge. The synthetic AN-hepc peptide showed a wide spectrum of antimicrobial activity in vitro toward gram-positive and gram-negative bacteria. In particular, this peptide demonstrated potent antimicrobial activity against the aquatic pathogens Vibrio alginolyticus, V. parahaemolyticus, V. vulnificus, Aeromonas hydrophila and Streptococcus agalactiae. The in vivo bacterial challenge results demonstrated that the synthetic AN-hepc peptide significantly improved the survival rate of S. agalactiae- and V. vulnificus-infected zebrafish. Taken together, these data indicate an important role for AN-hepc in the innate immunity of A. nigrofasciata and suggest its potential application in aquaculture for increasing resistance to disease.

Keywords

Hepcidin Amatitlania nigrofasciata Cloning Gene expression Antimicrobial activity 

Notes

Acknowledgments

We thank the Taiwan Zebrafish Core Facility at Academia Sinica (TZCAS), which is supported by a grant from the National Science Council (NSC 101-2321–B-001-026) in Taiwan, for providing the AB strain zebrafish. This research was supported by a grant from the National Science Council (NSC 102-2324-B-020-001-CC1). We appreciate Dr. Jyh-Yih Chen and Dr. Chung-Hung Liu for kindly providing the bacterial pathogens.

Supplementary material

10695_2014_9996_MOESM1_ESM.doc (1 mb)
Supplementary material 1 (DOC 1034 kb)

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Copyright information

© Springer Science+Business Media Dordrecht 2014

Authors and Affiliations

  1. 1.Department of Biochemical Science and TechnologyNational Taiwan UniversityTaipeiTaiwan
  2. 2.Institute of Cellular and Organismic BiologyAcademia SinicaTaipeiTaiwan
  3. 3.Department of Biological Science and TechnologyNational Pingtung University of Science and TechnologyNeipu, PingtungTaiwan

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