Fish Physiology and Biochemistry

, Volume 41, Issue 1, pp 243–253 | Cite as

Comparison of endogenous loss and maintenance need for minerals in rainbow trout (Oncorhynchus mykiss) fed fishmeal or plant ingredient-based diets

  • P. Antony Jesu Prabhu
  • S. J. Kaushik
  • C. Mariojouls
  • A. Surget
  • S. Fontagné-Dicharry
  • J. W. Schrama
  • I. Geurden


Mineral needs as affected by changes in dietary protein and oil sources were studied in rainbow trout. Duplicate groups (n = 30 fish per replicate) of rainbow trout (initial BW: 37 g) were fed either a fish meal/fish oil-based (M) or a complete plant ingredient (V)-based diet at four graded ration (R) levels [apparent satiation (AS), R75, R50 and R25 % of AS]; one treatment group was maintained under starvation. The feeding trial lasted 12 weeks at a water temperature of 17 °C. Dietary intake, apparent digestibility and initial and final whole-body composition data were used to calculate mineral gain which was regressed against digestible mineral intake (both expressed as mg or µg kg−0.8 day−1). Starvation loss (SL), endogenous loss of fed fish (ELF, y-intercept at x = 0) and point of intake for zero balance (PZB, x-intercept at y = 0) were used as estimates of maintenance requirements. SL provided the lowest estimate, ELF provided the net requirement of a mineral for maintenance and PZB provided the digestible dietary intake required to meet maintenance (SL < ELF < PZB). Dietary ingredient composition did not significantly affect the digestible mineral supply required for maintenance (PZB) for any of the minerals (P, Mg, K, Cu and Zn) studied. However, ELF of micro-minerals such as Cu and Zn were significantly affected. The ELF of Cu was significantly lower and that of Zn was significantly higher in V group compared with M-fed fish. Further studies on the effects of such changes in dietary formulations on micro-mineral metabolism are warranted.


Rainbow trout Dietary changes Minerals Endogenous loss Maintenance 



This work was part of a PhD thesis funded by INRA, under the INRA-WUR aquaculture platform; this work is also a contribution to the EU-funded project, ARRAINA: Advanced Research Initiatives for Nutrition & Aquaculture (KBBE-2011-288925). The efforts of F. Terrier, P. Aguirre and other staff at the INRA experimental fish farm are acknowledged.


  1. Antony Jesu Prabhu P et al (2014a) Post-prandial changes in plasma mineral levels in rainbow trout fed a complete plant ingredient based diet and the effect of supplemental di-calcium phosphate. Aquaculture 430:34–43CrossRefGoogle Scholar
  2. Antony Jesu Prabhu P, Schrama JW, Kaushik SJ (2014b) Mineral requirements of fish: a systematic review. Rev Aquac 6:1–48. doi: 10.1111/raq.12090 CrossRefGoogle Scholar
  3. Åsgård T, Shearer KD (1997) Dietary phosphorus requirement of juvenile Atlantic salmon, Salmo salar L. Aquac Nutr 3:17–23CrossRefGoogle Scholar
  4. Baker DH (1984) Equalized versus ad libitum feeding. Nutr Rev 42:269–273Google Scholar
  5. Bolin DW, King RP, Klosterman EW (1952) A simplified method for the determination of chromic oxide (Cr2O3) when used as an index substance. Science 116:634–635PubMedCrossRefGoogle Scholar
  6. Bureau D, Cho C (1999) Phosphorus utilization by rainbow trout (Oncorhynchus mykiss): estimation of dissolved phosphorus waste output. Aquaculture 179:127–140CrossRefGoogle Scholar
  7. Bureau DP, Hua K, Cho CY (2006) Effect of feeding level on growth and nutrient deposition in rainbow trout (Oncorhynchus mykiss Walbaum) growing from 150 to 600 g. Aqua Res 37:1090–1098Google Scholar
  8. Cho C, Kaushik S (1990) Nutritional energetics in fish: energy and protein utilization in rainbow trout (Salmo gairdneri). World Rev Nutr Diet 61:132–172Google Scholar
  9. Choubert G, De la Noüe J, Luquet P (1982) Digestibility in fish: improved device for the automatic collection of feces. Aquaculture 29:185–189CrossRefGoogle Scholar
  10. Cousins RJ (1985) Absorption, transport, and hepatic metabolism of copper and zinc: special reference to metallothionein and ceruloplasmin. Physiol Rev 65:238–309PubMedGoogle Scholar
  11. Cowey C (1992) Nutrition: estimating requirements of rainbow trout. Aquac 100:177–189Google Scholar
  12. Cowieson A, Acamovic T, Bedford M (2004) The effects of phytase and phytic acid on the loss of endogenous amino acids and minerals from broiler chickens. Br Poult Sci 45:101–108PubMedCrossRefGoogle Scholar
  13. Dabrowska H, Meyer-Burgdorff KH, Gunther KD (1991) Magnesium status in freshwater fish, common carp (Cyprinus carpio L.) and the dietary protein-magnesium interaction. Fish Physiol Biochem 9:165–172PubMedCrossRefGoogle Scholar
  14. Dilger RN, Adeola O (2006a) Estimation of true phosphorus digestibility and endogenous phosphorus loss in growing chicks fed conventional and low-phytate soybean meals. Poult Sci 85:661–668PubMedCrossRefGoogle Scholar
  15. Dilger RN, Adeola O (2006b) Estimation of true phosphorus digestibility and endogenous phosphorus loss in growing pigs fed conventional and low-phytate soybean meals. J Anim Sci 84:627–634PubMedGoogle Scholar
  16. Du C et al (2004) Dietary polyunsaturated fatty acids suppress acute hepatitis, alter gene expression and prolong survival of female Long-Evans Cinnamon rats, a model of Wilson disease. J Nutr Biochem 15:273–280PubMedCrossRefGoogle Scholar
  17. El-Mowafi A, Maage A (1998) Magnesium requirement of Atlantic salmon (Salmo salar L.) parr in seawater-treated fresh water. Aquac Nutr 4:31–38CrossRefGoogle Scholar
  18. El-Mowafi AFA, Maage A, Lorentzen M, Hassanein EI, Julshamn K (1997) Tissue indicators of element status in Atlantic salmon (Salmo salar) post-smolts: effect of fasting. Aquac Nutr 3:73–80CrossRefGoogle Scholar
  19. Fournier V, Gouillou-Coustans M, Metailler R, Vachot C, Guedes M, Tulli F, Oliva-Teles A, Tibaldi E, Kaushik S (2002) Protein and arginine requirements for maintenance and nitrogen gain in four teleosts. Br J Nutr 87:459–469Google Scholar
  20. Gahl MJ, Finke MD, Crenshaw TD, Benevenga NJ (1991) Use of a four-parameter logistic equation to evaluate the response of growing rats to ten levels of each indispensable amino acid. J Nutr 121:1720–1729PubMedGoogle Scholar
  21. Gatlin DM, Phillips HF (1989) Dietary calcium, phytate and zinc interactions in channel catfish. Aquaculture 79:259–266CrossRefGoogle Scholar
  22. Gatlin DM, Poe WE, Wilson RP (1986) Protein and energy requirements of fingerling channel catfish for maintenance and maximum growth. J Nutr 116:2121–2131Google Scholar
  23. Glencross B (2008) A factorial growth and feed utilization model for barramundi, Lates calcarifer based on Australian production conditions. Aquac Nutr 14:360–373Google Scholar
  24. Grisdale-Helland B, Gatlin DM, Corrent E, Helland SJ (2011) The minimum dietary lysine requirement, maintenance requirement and efficiency of lysine utilization for growth of Atlantic salmon smolts. Aquac Res 42:1509–1529CrossRefGoogle Scholar
  25. Gross JB Jr, Myers BM, Kost LJ, Kuntz S, LaRusso NF (1989) Biliary copper excretion by hepatocyte lysosomes in the rat. Major excretory pathway in experimental copper overload. J Clin Invest 83:30–39PubMedCentralPubMedCrossRefGoogle Scholar
  26. Gu M, Kortner TM, Penn M, Hansen AK, Krogdahl Å (2014) Effects of dietary plant meal and soya-saponin supplementation on intestinal and hepatic lipid droplet accumulation and lipoprotein and sterol metabolism in Atlantic salmon (Salmo salar L.). Br J Nutr 111:432–444PubMedCrossRefGoogle Scholar
  27. Han D, Liu H, Liu M, Xiao X, Zhu X, Yang Y, Xie S (2012) Effect of dietary magnesium supplementation on the growth performance of juvenile gibel carp, Carassius auratus gibelio. Aquac Nutr 18:512–520CrossRefGoogle Scholar
  28. Hardy RW, Shearer KD (1985) Effect of dietary calcium phosphate and zinc supplementation on whole body zinc concentration of rainbow trout (Salmo gairdneri). Can J Fish Aquat Sci 42:181–184CrossRefGoogle Scholar
  29. Helland SJ, Hatlen B, Grisdale-Helland B (2010) Energy, protein and amino acid requirements for maintenance and efficiency of utilization for growth of Atlantic salmon post-smolts determined using increasing ration levels. Aquac 305:150–158Google Scholar
  30. Ketola HG, Richmond ME (1994) Requirement of rainbow trout for dietary phosphorus and its relationship to the amount discharged in hatchery effluents. Trans Am Fish Soc 123:587–594CrossRefGoogle Scholar
  31. Kortner TM, Gu J, Krogdahl Å, Bakke AM (2013) Transcriptional regulation of cholesterol and bile acid metabolism after dietary soyabean meal treatment in Atlantic salmon (Salmo salar L.). Br J Nutr 109:593–604PubMedCrossRefGoogle Scholar
  32. Kousoulaki K, Fjelldal PG, Aksnes A, Albrektsen S (2010) Growth and tissue mineralisation of Atlantic cod (Gadus Morhua) fed soluble P and Ca salts in the diet. Aquaculture 309:181–192CrossRefGoogle Scholar
  33. Lanno RP, Hicks B, Hilton JW (1987) Histological observations on intrahepatocytic copper-containing granules in rainbow trout reared on diets containing elevated levels of copper. Aquat Toxicol 10:251–263CrossRefGoogle Scholar
  34. Levy R, Herzberg GR (1995) Effects of dietary fish oil and corn oil on bile flow and composition in rats. Nutr Res 15:85–98CrossRefGoogle Scholar
  35. Levy R, Herzberg GR (1996) Effects of a meal of fish oil or corn oil on bile flow and composition in rats previously adapted to diets containing fish oil or corn oil. Nutr Res 16:805–816CrossRefGoogle Scholar
  36. Liang JJ, Yang HJ, Liu YJ, Tian LX (2012a) Dietary potassium requirement of juvenile grass carp (Ctenopharyngodon idella Val.) based on growth and tissue potassium content. Aquac Res 45:701–708CrossRefGoogle Scholar
  37. Liang JJ, Yang HJ, Liu YJ, Tian LX (2012b) Dietary magnesium requirement and effects on growth and tissue magnesium content of juvenile grass carp (Ctenopharyngodon idella). Aquac Nutr 18:56–64CrossRefGoogle Scholar
  38. Lupatsch I, Kissil GW, Sklan D, Pfeffer E (1998) Energy and protein requirements for maintenance and growth in gilthead seabream (Sparus aurata L.). Aquac Nutr 4:165–173Google Scholar
  39. McClain W, Gatlin DM (1988) Dietary zinc requirement of Oreochromis aureus and effects of dietary calcium and phytate on zinc bioavailability. J World Aquac Soc 19:103–108CrossRefGoogle Scholar
  40. Mitchell H, Edman M (1962) Nutritional significance of the dermal losses of nutrients in man, particularly of nitrogen and minerals. Am J Clin Nutr 10:163–172Google Scholar
  41. NRC (2011) Nutrient requirements of fish and shrimp. National Research Council, The National Academies Press, Washington, D.CGoogle Scholar
  42. Ogino C, Takeda H (1978) Mineral requirements in fish. 5. Requirements of rainbow-trout for dietary calcium and phosphorus. Bull Jap Soc Sci Fish 44:1019–1022CrossRefGoogle Scholar
  43. Ojo AA, Nadella SR, Wood CM (2009) In vitro examination of interactions between copper and zinc uptake via the gastrointestinal tract of the rainbow trout (Oncorhynchus mykiss). Arch Environ Contam Toxicol 56:244–252PubMedCrossRefGoogle Scholar
  44. Pfeffer E, Pieper A (1979) Application of factorial approach for deriving nutrient requirements of rainbow trout. In: Halver JE, Tlews KT (eds) Fish nutrition and fishfeed technology No. 2. Berlin, p 545–553Google Scholar
  45. Pfeffer E, Potthast V (1977) Studies on the use of energy, protein and mineral elements in growth of rainbow trout. Fortschr Tierphysiol Tierernahr 8:32–55PubMedGoogle Scholar
  46. Ramseyer L, Garling D, Hill G, Link J (1999) Effect of dietary zinc supplementation and phytase pre-treatment of soybean meal or corn gluten meal on growth, zinc status and zinc-related metabolism in rainbow trout, Oncorhynchus mykiss. Fish Physiol Biochem 20:251–261CrossRefGoogle Scholar
  47. Richardson NL, Higgs DA, Beames RM, McBride JR (1985) Influence of dietary calcium, phosphorus, zinc and sodium phytate level on cataract incidence, growth and histopathology in juvenile chinook salmon (Oncorhynchus tshawytscha). J Nutr 115:553–567PubMedGoogle Scholar
  48. Rodehutscord M (1996) Response of rainbow trout (Oncorhynchus mykiss) growing from 50 to 200 g to supplements of dibasic sodium phosphate in a semipurified diet. J Nutr 126:324–331PubMedGoogle Scholar
  49. Rodehutscord M, Pfeffer E (1999) Maintenance requirement for digestible energy and efficiency of utilisation of digestible energy for retention in rainbow trout, Oncorhynchus mykiss. Aquac 179:95–107Google Scholar
  50. Roelofsen H, Wolters H, Van Luyn MJA, Miura N, Kuipers F, Vonk RJ (2000) Copper-induced apical trafficking of ATP7B in polarized hepatoma cells provides a mechanism for biliary copper excretion. Gastroenterology 119:782–793PubMedCrossRefGoogle Scholar
  51. Roy PK, Lall SP (2004) Urinary phosphorus excretion in haddock, Melanogrammus aeglefinus (L.) and Atlantic salmon, Salmo salar (L.). Aquaculture 233:369–382CrossRefGoogle Scholar
  52. Satoh S, Poe WE, Wilson RP (1989) Effect of supplemental phytate and/or tricalcium phosphate on weight gain, feed efficiency and zinc content in vertebrae of channel catfish. Aquaculture 80:155–161CrossRefGoogle Scholar
  53. Satoh S, Takeuchi T, Watanabe T (1991) Availability of manganese and magnesium contained in white fish meal to rainbow trout Oncorhynchus mykiss. Nippon Suisan Gakk 57:99–104CrossRefGoogle Scholar
  54. Schulin-Zeuthen M, Kebreab E, Gerrits WJJ, Lopez S, Fan MZ, Dias RS, France J (2007) Meta-analysis of phosphorus balance data from growing pigs. J Anim Sci 85:1953–1961PubMedCrossRefGoogle Scholar
  55. Shearer KD (1988) Dietary potassium requirement of juvenile Chinook salmon. Aquaculture 73:119–129CrossRefGoogle Scholar
  56. Shearer KD (1989) Whole body magnesium concentration as an indicator of magnesium status in rainbow trout (Salmo gairdneri). Aquaculture 77:201–210CrossRefGoogle Scholar
  57. Shearer KD (1995) The use of factorial modeling to determine the dietary requirements for essential elements in fishes. Aquac 133:57–72Google Scholar
  58. Shearer KD, Åsgård T (1990) Availability of dietary magnesium to rainbow trout as determined by apparent retention. Aquaculture 86:51–61CrossRefGoogle Scholar
  59. Shearer KD, Åsgård T (1992) The effect of water-borne magnesium on the dietary magnesium requirement of the rainbow trout (Oncorhynchus mykiss). Fish Physiol Biochem 9:387–392PubMedCrossRefGoogle Scholar
  60. Shiau SY, Hsieh JF (2001) Quantifying the dietary potassium requirement of juvenile hybrid tilapia (Oreochromis niloticus × O. aureaus). Br J Nutr 85:213–218PubMedCrossRefGoogle Scholar
  61. Skonberg DI, Yogev L, Hardy RW, Dong FM (1997) Metabolic response to dietary phosphorus intake in rainbow trout (Oncorhynchus mykiss). Aquaculture 157:11–24CrossRefGoogle Scholar
  62. Sugiura SH, Dong FM, Rathbone CK, Hardy RW (1998) Apparent protein digestibility and mineral availabilities in various feed ingredients for salmonid feeds. Aquaculture 159:177–202CrossRefGoogle Scholar
  63. Sugiura SH, Dong FM, Hardy RW (2000) A new approach to estimating the minimum dietary requirement of phosphorus for large rainbow trout based on nonfecal excretions of phosphorus and nitrogen. J Nutr 130:865–872PubMedGoogle Scholar
  64. Vielma J, Lall SP (1998) Control of phosphorus homeostasis of Atlantic salmon (Salmo salar) in fresh water. Fish Physiol Biochem 19:83–93CrossRefGoogle Scholar
  65. Vilhelmsson OT, Martin SAM, Médale F, Kaushik SJ, Houlihan DF (2004) Dietary plant-protein substitution affects hepatic metabolism in rainbow trout (Oncorhynchus mykiss). Br J Nutr 92:71–80PubMedCrossRefGoogle Scholar
  66. Wilson RP, Naggar GE (1992) Potassium requirement of fingerling channel catfish, Ictalurus puntatus. Aquaculture 108:169–175CrossRefGoogle Scholar
  67. Woyengo TA, Cowieson AJ, Adeola O, Nyachoti CM (2009) Ileal digestibility and endogenous flow of minerals and amino acids: responses to dietary phytic acid in piglets. Br J Nutr 102:428–433PubMedCrossRefGoogle Scholar
  68. Yamamoto T, Suzuki N, Furuita H, Sugita T, Tanaka N, Goto T (2007) Supplemental effect of bile salts to soybean meal-based diet on growth and feed utilization of rainbow trout Oncorhynchus mykiss. Fish Sci 73:123–131CrossRefGoogle Scholar

Copyright information

© Springer Science+Business Media Dordrecht 2014

Authors and Affiliations

  • P. Antony Jesu Prabhu
    • 1
    • 2
    • 3
  • S. J. Kaushik
    • 1
  • C. Mariojouls
    • 3
  • A. Surget
    • 1
  • S. Fontagné-Dicharry
    • 1
  • J. W. Schrama
    • 2
  • I. Geurden
    • 1
  1. 1.INRA, UR 1067, Nutrition, Metabolism and Aquaculture (NuMeA), AquapôleSaint-Pee-Sur-NivelleFrance
  2. 2.AFI, WIASWageningen UniversityWageningenThe Netherlands
  3. 3.AgroParisTechParis, Cedex 5France

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