Fish Physiology and Biochemistry

, Volume 40, Issue 2, pp 355–363 | Cite as

Effects of dietary selenium of organic form against lead toxicity on the antioxidant system in Cyprinus carpio

  • Ferbal Özkan-Yılmaz
  • Arzu Özlüer-Hunt
  • Suna Gül Gündüz
  • Mehmet Berköz
  • Serap Yalın


In this study was evaluated potential protective effect of organic selenium (Se) on heavy metal stress induced by lead (Pb) in Cyprinus carpio. For this reason, C. carpio was exposed to sublethal concentration of Pb (1.5 mg/L Pb(NO3)2) for 14 days. The fish were fed a basal (control; measured 0.55 mg/kg Se) diet or a basal diet supplemented with 2.50 mg/kg (measured 2.92 mg/kg Se) organic Se (Sel-Plex®) during the experiment period. The variations in glutathione peroxidase (GSH-Px), glutathione S-transferase (GST) activities, and levels of reduced glutathione (GSH) with malondialdehyde (MDA) in liver and brain tissues of C. carpio were investigated in experimental groups. GSH levels in liver and brain tissues were significantly decreased by exposure to Pb. GST activity was significantly increased (p < 0.05) in liver tissue, but decreased in brain of treated fish by exposure to Pb. Also, GSH-Px activity was significantly increased in liver tissue, but decreased in brain of Pb-treated fish. Levels of MDA were increased in liver and brain of Pb-treated fish. The organic Se treatment for Pb-intoxicated animals improved activities of GSH-Px, GST and levels of MDA within normal limits. Supplemented Se could be able to improve Pb-induced oxidative stress by decreasing lipid peroxidation and regulating antioxidant defense system in tissues.


Cyprinus carpio Selenium yeast Lead Antioxidant enzyme 


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Copyright information

© Springer Science+Business Media Dordrecht 2013

Authors and Affiliations

  • Ferbal Özkan-Yılmaz
    • 1
  • Arzu Özlüer-Hunt
    • 1
  • Suna Gül Gündüz
    • 1
  • Mehmet Berköz
    • 2
  • Serap Yalın
    • 3
  1. 1.Department of Basic Sciences, Faculty of FisheriesMersin UniversityMersinTurkey
  2. 2.Faculty of PharmacyYüzüncü Yıl UniversityVanTurkey
  3. 3.Faculty of PharmacyMersin UniversityMersinTurkey

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