Rearing temperature affects Senegalese sole (Solea senegalensis) larvae protein metabolic capacity
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The present work examined the short- and long-term effects of three rearing temperatures on protein metabolism and growth trajectories of Senegalese sole larvae using 14C-labelled Artemia protein as feed. A first feeding trial was performed on larvae reared at 15, 18 and 21 °C (at 26, 17 and 14 days post-hatching (dph), respectively) and a second trial conducted on post-larvae after transfer to the same rearing temperature (~20 °C) (49, 35 and 27 dph, in larvae initially reared at 15, 18 and 21 °C, respectively). Temperature greatly influenced larvae relative growth rate (RGR) and survival, since growth at 15 °C was severely depressed. Protein digestibility and retention was highest at 18 °C during the first trial (85.35 ± 1.16 and 86.34 ± 2.33 %, respectively). However, during the second trial, post-larvae from 15 °C had the highest feed intake and protein digestibility (3.58 ± 1.54 and 75.50 ± 1.35 %, respectively), although retention was similar between treatments. Furthermore, after transfer to 20 °C larvae from 15 °C experienced compensatory growth, which was observed until 121 dph, and confirmed by RGR values, which were significantly higher at 15 ºC than at 21 ºC or 18 ºC. Results from the present study show that Solea senegalensis larval development, survival and protein digestion and retention are highly affected by thermal history.
KeywordsRearing temperature Artemia intake Protein digestibility Retention efficiency Compensatory growth Solea senegalensis
The authors would like to greatly acknowledge J. Sendão, H. Teixeira, F. Rocha and A. Santos from CCMAR (Portugal), for their invaluable help during the experimental set-up and sampling of Senegalese sole larvae. This work was funded by Project EPISOLE [PTDC/MAR/110547/2009] from Fundação para a Ciência e a Tecnologia (FCT), Portugal. C. Campos and S. Engrola acknowledge the financial support by Fundação para a Ciência e Tecnologia, Portugal, through grants SFRH/BD/43633/2008 and SFRH/BPD/49051/2008, respectively.
- Costas B, Aragao C, Ruiz-Jarabo I, Vargas-Chacoff L, Arjona FJ, Mancera JM, Dinis MT, Conceição LE (2011) Different environmental temperatures affect amino acid metabolism in the eurytherm teleost Senegalese sole (Solea senegalensis Kaup, 1858) as indicated by changes in plasma metabolites. Amino Acids. doi: 10.1007/s00726-011-1082-0 PubMedGoogle Scholar
- Dinis MT (1986) Quatre Soleidae de léstuaire du Tage. Reprodution et croissance. Essay d’elevage de Solea senegalensis Kaup. Université de Bretagne Occidentale, BrestGoogle Scholar
- Houde ED (1989) Comparative growth, mortality, and energetics of marine fish larvae—temperature and implied latitudinal effects. Fish B-Noaa 87(3):471–495Google Scholar
- Johnston IA, Hall TE (2004) Mechanisms of muscle development and responses to temperature change in fish larvae. In: Govoni JJ (ed) The development of form and function in fishes and the question of larval adaptation, vol 40. American Fisheries Society Symp, Bethesda, Maryland, pp 113–144Google Scholar
- Mai MG, Engrola S, Morais S, Portella MC, Verani JR, Dinis MT, Conceicao LEC (2009) Co-feeding of live feed and inert diet from first-feeding affects Artemia lipid digestibility and retention in Senegalese sole (Solea senegalensis) larvae. Aquaculture 296(3–4):284–291. doi: 10.1016/j.aquaculture.2009.08.024 CrossRefGoogle Scholar
- Morais S, Lacuisse M, Conceição LEC, Dinis MT, Rønnestad I (2004b) Ontogeny of the digestive capacity of Senegalese sole (Solea senegalensis), with respect to digestion, absorption and metabolism of amino acids from Artemia. Mar Biol 145(2):243–250. doi: 10.1007/s00227-004-1326-6 CrossRefGoogle Scholar
- Mylonas C, Anezaki L, Divanach P, Zanuy S, Piferrer F, Ron B, Peduel A, Ben Atia I, Gorshkov S, Tandler A (2005) Influence of rearing temperature during the larval and nursery periods on growth and sex differentiation in two Mediterranean strains of Dicentrarchus labrax. J Fish Biol 67:652–668CrossRefGoogle Scholar
- Ricker WE (1958) Handbook of computations for biological statistics of fish populations. Bull Fish Res Board Can 119:1–300Google Scholar
- Rønnestad I, Conceição L (2005) Aspects of protein and amino acid digestion and utilization by marine fish larvae. In: Starck JM, Wang T (eds) Physiological and ecological adaptations to feeding in vertebrates. Science Publishers, Enfield, NH, USA, pp 389–416Google Scholar
- Rønnestad I, Tonheim SK, Fyhn HJ, Rojas-García CR, Kamisaka Y, Koven W, Finn RN, Terjesen BF, Barr Y, Conceição LEC (2003) The supply of amino acids during early feeding stages of marine fish larvae: a review of recent findings. Aquaculture 227(1–4):147–164. doi: 10.1016/s0044-8486(03)00500-3 CrossRefGoogle Scholar