Partial characterization and ontogenetic development of pancreatic digestive enzymes in Japanese eel Anguilla japonica larvae
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The pancreatic digestive enzymes, trypsin, chymotrypsin, lipase and amylase were partially characterized, and changes in their activities were examined during the initial ontogeny of Japanese eel Anguilla japonica larvae from 5 to 34 days post-hatching (dph). The pH optima of the eel larval enzymes were narrower than those other fish species; trypsin activity was highest at pH 9, chymotrypsin and amylase activities were highest at pH 7 and 8, and lipase activity was highest at pH 8 and 9. In an analysis of thermal profiles, the larval pancreatic enzymes had a high optimal temperature and high thermal stability, which are typical of fish from the tropics. At 12 and 13 dph, lipase activity and gene expression levels of trypsin (-a and -b), lipase and amylase decreased markedly, suggesting a marked change in larval metabolism at that time. These data could be useful in the development of artificial larval diets in Japanese eel.
KeywordsJapanese eel Larvae Pancreatic digestive enzymes Activity Gene expression
We are grateful to Dr. Y. Masuda, Mr. H. Imaizumi, Mr. T. Jinbo (Sibushi Laboratory, NRIA) and Mr. H. Hashimoto (Yaeyama Laboratory, Seikai National Fisheries Research Institute) for providing the Japanese eel larvae. This study was supported by a grant-in-aid for “Development of Sustainable Aquaculture Technology Independent of Wild Fishery Resources” from the Ministry of Agriculture, Forestry and Fisheries, Government of Japan.
- Alvarez-González CA, Cervantes-Trujano M, Tovar-Ramírez D, Conklin D, Nolasco H, Gisbert E, Piedrahita R (2006) Development of digestive enzymes in California halibut Paralichthys californicus larvae. Fish Physiol Biochem 31:83–93Google Scholar
- Castillo-Yáñez FJ, Pacheco-Aguilar R, Lugo-Sanchez ME, Garcia-Sanchez G, Quintero-Reyes IE (2009) Biochemical characterization of an isoform of chymotrypsin from the viscera of Monterey sardine (Sardinops sagax caerulea), and comparison with bovine chymotrypsin. Food Chem 112:634–639CrossRefGoogle Scholar
- Geiger R (1986) Chymotrypsin. VCH, WeinheimGoogle Scholar
- Jimenez-Martinez LD, Alvarez-González CA, Tovar-Ramírez D, Gaxiola G, Sanchez-Zamora A, Moyano FJ, Alarcón FJ, Márquez-Couturier G, Gisbert E, Contreras-Sánchez WM, Perales-García N, Arias-Rodríguez L, Indy JR, Páramo-Delgadillo S, Palomino-Albarrán IG (2012) Digestive enzyme activities during early ontogeny in Common snook (Centropomus undecimalis). Fish Physiol Biochem 38:441–454CrossRefPubMedGoogle Scholar
- Kagawa H, Tanaka H, Ohta H, Okuzawa K, Iinuma N (1997) Induced ovulation by injection of 17,20 beta-dihydroxy-4-pregnen-3-one in the artificially matured Japanese eel, with special reference to ovulation time. Fish Sci 63:365–367Google Scholar
- Kurokawa T, Koshio M, Kaiya H, Hashimoto H, Nomura K, Uji S, Awaji M, Gen K, Tanaka H (2011) Distribution of pepsinogen- and ghrelin-producing cells in the digestive tract of Japanese eel (Anguilla japonica) during metamorphosis and the adult stage. Gen Comp Endocrinol 173:475–482CrossRefPubMedGoogle Scholar
- Ohta H, Kagawa H, Tanaka H, Okuzawa K, Hirose K (1996) Milt production in the Japanese eel Anguilla japonica induced by repeated injections of human chorionic gonadotropin. Fish Sci 62:44–49Google Scholar
- Oozeki Y, Bailey K (1995) Ontogenetic development of digestive enzyme-activities in larval walleye pollock, Theragra chalcogramma. Mar Biol 122:177–186Google Scholar
- Tsukamoto K, Chow S, Otake T, Kurogi H, Mochioka N, Miller MJ, Aoyama J, Kimura S, Watanabe S, Yoshinaga T, Shinoda A, Kuroki M, Oya M, Watanabe T, Hata K, Ijiri S, Kazeto Y, Nomura K, Tanaka H (2011) Oceanic spawning ecology of freshwater eels in the western North Pacific. Nat Commun 2:179CrossRefPubMedGoogle Scholar