Fish Physiology and Biochemistry

, Volume 39, Issue 4, pp 895–905 | Cite as

Partial characterization and ontogenetic development of pancreatic digestive enzymes in Japanese eel Anguilla japonica larvae

  • Koji Murashita
  • Hirofumi Furuita
  • Hiroyuki Matsunari
  • Takeshi Yamamoto
  • Masahiko Awaji
  • Kazuharu Nomura
  • Jiro Nagao
  • Hideki Tanaka
Article

Abstract

The pancreatic digestive enzymes, trypsin, chymotrypsin, lipase and amylase were partially characterized, and changes in their activities were examined during the initial ontogeny of Japanese eel Anguilla japonica larvae from 5 to 34 days post-hatching (dph). The pH optima of the eel larval enzymes were narrower than those other fish species; trypsin activity was highest at pH 9, chymotrypsin and amylase activities were highest at pH 7 and 8, and lipase activity was highest at pH 8 and 9. In an analysis of thermal profiles, the larval pancreatic enzymes had a high optimal temperature and high thermal stability, which are typical of fish from the tropics. At 12 and 13 dph, lipase activity and gene expression levels of trypsin (-a and -b), lipase and amylase decreased markedly, suggesting a marked change in larval metabolism at that time. These data could be useful in the development of artificial larval diets in Japanese eel.

Keywords

Japanese eel Larvae Pancreatic digestive enzymes Activity Gene expression 

Notes

Acknowledgments

We are grateful to Dr. Y. Masuda, Mr. H. Imaizumi, Mr. T. Jinbo (Sibushi Laboratory, NRIA) and Mr. H. Hashimoto (Yaeyama Laboratory, Seikai National Fisheries Research Institute) for providing the Japanese eel larvae. This study was supported by a grant-in-aid for “Development of Sustainable Aquaculture Technology Independent of Wild Fishery Resources” from the Ministry of Agriculture, Forestry and Fisheries, Government of Japan.

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Copyright information

© Springer Science+Business Media Dordrecht 2012

Authors and Affiliations

  • Koji Murashita
    • 1
  • Hirofumi Furuita
    • 1
  • Hiroyuki Matsunari
    • 1
  • Takeshi Yamamoto
    • 1
  • Masahiko Awaji
    • 1
  • Kazuharu Nomura
    • 1
  • Jiro Nagao
    • 1
  • Hideki Tanaka
    • 1
  1. 1.National Research Institute of AquacultureFisheries Research AgencyTamaki, MieJapan

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