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Fish Physiology and Biochemistry

, Volume 38, Issue 4, pp 1107–1116 | Cite as

Cytochrome P4501A mRNA and protein induction in striped bass (Morone saxatilis)

  • Eric D. H. DurieuxEmail author
  • Richard E. Connon
  • Inge Werner
  • Leandro S. D’Abronzo
  • Patrick S. Fitzgerald
  • Jimmy L. Spearow
  • David J. Ostrach
Article

Abstract

The striped bass (Morone saxatilis) supports a valuable recreational fishery and is among the most important piscivorous fish of the San Francisco Estuary. This species has suffered a significant decline in numbers over the past decades, and there is indication that contaminants are important contributors. Polycyclic aromatic hydrocarbons (PAHs) and polyhalogenated aromatic hydrocarbons (PHAHs) including PCBs and dioxins are widespread in the estuary, they typically bioaccumulate through trophic levels, reaching highest levels in top predators and are known to affect the fish health and development. The aim of this study was to investigate the dynamics of cytochrome P4501A (Cyp1a) induction simultaneously at different levels of biological organization (RNA transcription and protein synthesis) as a biomarker of exposure to PAHs and PHAHs. We utilized β-naphthoflavone (BNF) as a model PAH to induce Cyp1a responses in juvenile striped bass in both dose–response and time–response assessments and determined Cyp1a mRNA and protein levels. Significant responses were measured in both systems at 10 mg ΒΝF kg−1, a concentration used for time–response studies. Messenger RNA levels peaked at 6 h post-injection, while protein levels increased progressively with time, significantly peaking at 96 h post-injection; both remaining elevated throughout the duration of the test (8 days). Our data suggest that rapid induction of gene transcription following exposure and subsequent cumulative protein synthesis could provide a useful means of identifying temporal variants in exposure to Cyp1a inducers in Morone saxatilis. The potential application of this combined Cyp1a gene and protein biomarker in this species for field studies is discussed.

Keywords

Cyp1a Biomarker Fish Morone saxatilis β-Naphthoflavone Polycyclic aromatic hydrocarbon 

Notes

Acknowledgments

Funding for this study was provided by the Interagency Ecological Program on Pelagic Organism Decline Investigations, Department of Water Resources, Sacramento, California, contract Numbers 4600004664 to D. J. Ostrach, and 4600004445 to I. Werner, with special thanks to Ted Sommer and Fred Feyrer. We thank Dr. Moshe Tom for providing his modified bacteria, advice and encouragement. This study was part of E. D. H. Durieux’s post-doctorate in ecotoxicology at UC Davis in the laboratory of and mentored by D. J. Ostrach. We thank members of the Aquatic Toxicology Laboratory, and Center for Aquatic Biology and Aquaculture; University of California, Davis, for assistance and use of facilities. The opinions expressed in this paper are the authors and do not represent the opinions or policies of the California EPA or Department of Toxic Substances Control.

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Copyright information

© Springer Science+Business Media B.V. 2012

Authors and Affiliations

  • Eric D. H. Durieux
    • 1
    • 3
    Email author
  • Richard E. Connon
    • 2
  • Inge Werner
    • 2
    • 4
  • Leandro S. D’Abronzo
    • 2
    • 5
  • Patrick S. Fitzgerald
    • 1
  • Jimmy L. Spearow
    • 1
    • 6
  • David J. Ostrach
    • 1
    • 7
  1. 1.Pathobiology, Conservation and Population Biology Laboratory, John Muir Institute of the Environment, Center for Watershed SciencesUniversity of CaliforniaDavisUSA
  2. 2.Aquatic Toxicology Laboratory, School of Veterinary Medicine, Department of Anatomy, Physiology and Cell BiologyUniversity of CaliforniaDavisUSA
  3. 3.UMR 6134 Sciences Pour l’EnvironnementUniversité de Corse Pasquale PaoliCorteFrance
  4. 4.Swiss Centre for Applied Ecotoxicology, Eawag/EPFLDübendorfSwitzerland
  5. 5.University of California Medical CenterMatherUSA
  6. 6.Department of Toxic Substances ControlSacramentoUSA
  7. 7.Ostrach ConsultingWoodlandUSA

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