Fish Physiology and Biochemistry

, Volume 38, Issue 3, pp 725–734 | Cite as

Iodine and selenium supplementation increased survival and changed thyroid hormone status in Senegalese sole (Solea senegalensis) larvae reared in a recirculation system

  • A. R. A. Ribeiro
  • L. Ribeiro
  • Ø. Sæle
  • M. T. Dinis
  • M. Moren
Article
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Abstract

To test how iodine and both iodine and selenium supplementation affected the thyroid status as well as growth and survival in Senegalese sole, larvae were reared in a recirculation system from 15 to 34 DAH. Sets of three tanks were assigned to each of the following three diets: control (C), iodine (I) and iodine and selenium (I + Se). Samples were collected at 15, 27 and 34 DAH to determine dry weight, iodine and selenium levels, GPx and ORD activities, thyroid hormone levels and thyroid follicles histology. At 34 DAH, fish from the control (C) treatment suffered from hyperplasia of the thyroid follicles (goitre), whereas iodine-treated larvae did not (I and I + Se). Lower survival rates in the C groups were probably a consequence of the hyperplasia. Moreover, there was an improvement in thyroid hormone status in I- and I + Se-treated larvae, showing that further supplementation of live feed with iodine can be crucial for fish at early life stages, as it seems to sustain normal larval development, when reared in a recirculation system. Selenium did not affect the results. Together with previous results, this indicates selenium supplement is more important at younger life stages.

Keywords

Thyroid hormones Iodine Selenium Outer-ring deiodinase Glutathione peroxidase Flatfish Recirculation system 
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Notes

Acknowledgments

Special thanks to senior scientist Dr. Kristin Hamre for her inputs and comments which made possible the accomplishment of this project. This work was supported by the DIGFISH project, POCI/CVT/58790/2004 (FCT, Portugal). A. R. A. Ribeiro and L. Ribeiro benefit from grants SFRH/BD/24803/2005 and SFRH/BPD/7148/2001 (FCT, Portugal), respectively. We are indebted to the highly skilled technicians at CCMAR and NIFES.

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Copyright information

© Springer Science+Business Media B.V. 2011

Authors and Affiliations

  • A. R. A. Ribeiro
    • 1
  • L. Ribeiro
    • 1
  • Ø. Sæle
    • 2
  • M. T. Dinis
    • 1
  • M. Moren
    • 2
  1. 1.CCMAR—Universidade do AlgarveFaroPortugal
  2. 2.NIFES, National Institute of Nutrition and Seafood ResearchBergenNorway

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